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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Neuroscience 2024Quote: ... ASAP3 construct was cloned in pcDNA3.1/Puro-CAG plasmid backbone and used to transfect HEK293 cells with Lipofectamine 3000 (ThermoFisher, US) using 400 ng DNA ...
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bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Biochemistry 2023Quote: ... To generate stable expression cell lines Flp-In T-Rex HEK293 were grown in 100 μg/ml Zeocin (Invitrogen, R25001) for at least a week before transfection ...
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bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting constructs were integrated into HEK293 Flp-In T-REx cells using the Flp-In technology (Thermo Fisher Scientific). HEK293 Flp-In T-REx were seeded in 6-well plates and co-transfected the following day with 200 ng bait-BirA*-FLAG construct and 2 μg pOG44 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2024Quote: HEK293 (XX female) cells and HepG2 cells were cultured in high glucose Dulbecco’s modified eagle medium (DMEM) (41965, Thermo Fisher) and supplemented with 10% fetal bovine serum (10270 ...
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bioRxiv - Molecular Biology 2021Quote: ... The whole tag-attached DRD1 was then cloned into the standard pFastBac (Thermo Fisher) vector ...
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bioRxiv - Developmental Biology 2019Quote: ... which includes both the nuclear H2B-iRFP tag and cell tracker dye (Invitrogen; C12881), Blue and Infra-red fluorescent filter cubes were also used.
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with either biotinylated anti-C-tag conjugate (ThermoFisher, Cat. No. 7103252100) for the detection of baits ...
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bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
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bioRxiv - Cell Biology 2021Quote: ... Each sample was labeled with TMT 6 Plex Mass Tag Labeling Kit (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... or 1:7,500 diluted T7-tag polyclonal antibody-HRP (Thermo Fisher, cat# PA1-31449) were incubated with the plate for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) ( ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Pathology 2019Quote: ... Cell lysates were then incubated with mouse anti-GFP-tag antibody (MA5-15256, ThermoFisher) for 1 hr followed by 40 μL of protein A/G-magnetic beads for another 1 hr at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Tandem mass tag (TMT) ten-plex reagents (0.2 mg) (Thermo Fisher Scientific, Waltham, MA) were removed from −20 °C and allowed to reach room temperature prior to dissolving each tag in 20 µL of acetonitrile ...
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bioRxiv - Pathology 2021Quote: ... 0.1 % Tween20) and incubated with antibodies specific for V5 tag (ThermoFisher Scientific, MA, USA), PBX1 (Abnova ...
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... with an N-terminal FLAG- or HA-tag (16) or into pcDNA4/TO (Invitrogen) with an N-terminal FLAG- or EGFP-tag (17) ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg aliquots were next labeled using tandem mass tags (TMT) (Thermo Scientific, 90309). Dried peptides were resuspended in a solution of 30% dry acetonitrile (ACN ...
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bioRxiv - Genetics 2021Quote: ... Myc-Tag or PCNA signal was detected using SuperSignal West Femto (Thermo Fisher Scientific) or Clarity (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The V5 epitope tag was detected by using mouse monoclonal anti-V5 antibody (Invitrogen) at 1 ug/ml ...
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bioRxiv - Immunology 2022Quote: ... the CaptureSelect™ Alexa Fluor™ 488 anti-C-tag conjugate (Thermo Fisher Scientific) was added to the cell supernatant at a final dilution of 1:480 ...
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bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Immunology 2024Quote: ... The proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... anti-HA epitope tag (#901513) and Alexa Fluor 647 anti-mouse IgG (Invitrogen #A21235).
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bioRxiv - Bioengineering 2023Quote: ... MCF7 cells were transiently transfected with Premo FUCCI cell cycle sensor tags (Thermofisher, USA). MCF7 cells required additional treatment with BacMam enhancer (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Neuroscience 2022Quote: ... streptavidin conjugated to an Alexa Fluor™ (AF) fluorescent tag (1:400; Thermo Fisher Scientific ...