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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and mounted using Fluoromount-G (00-4958-02, Invitrogen, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min with PBS and incubated over-night at +4°C with goat anti-rabbit Alexa Fluor 564 (1:1000, Invitrogen, ThermoFisher Scientific) and goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 7 min with PBS and incubated overnight at +4°C with goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488 (each 1:1000, Invitrogen, ThermoFisher Scientific) in carrier solution ...
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bioRxiv - Neuroscience 2024Quote: ... The electrodes were labeled with DiI (1,1’-dioctadecyl-3,3,3’,3’-tetramethyl indocarbocyanine, Invitrogen) to enable reconstruction of the electrode tracks post mortem (Fig ...
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bioRxiv - Pathology 2024Quote: ... RNA was reverse transcribed using Superscript II (Invitrogen, Cat#18064014). The cDNA was amplified with 20 cycles and cleaned up with AMPure XP beads (Beckman Coulter Cat#A63881) ...
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bioRxiv - Pathology 2024Quote: ... using Zeba spin desalting columns (7K MWCO, 0.5 mL, Thermo Fisher Scientific), according to the manufacturers’ protocol ...
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bioRxiv - Pathology 2024Quote: ... in a T25 culture flask (Nunc) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Pathology 2024Quote: ... and 25 µL per well 0.06 µg/mL streptavidin horseradish peroxidase conjugate (Thermo Scientific) was added ...
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bioRxiv - Pathology 2024Quote: ... 1 ×106 C2C12 cells (ATCC® CRL-1772™) were seeded in 5 mL growth medium (high glucose Dulbecco’s Modified Eagle’s Medium (Gibco), 20% fetal bovine serum (Gibco ...
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bioRxiv - Pathology 2024Quote: ... in phenol-free Hank’s Buffered Salt Solution (HBSS, 14175095, Gibco) and cut 250 µm thick sections using a vibratome (VT1200S ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... utilizing SYBR Green PCR Master Mix reagents (Applied Biosystems REF 4367659) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... 25 µL Lipofectamine 2000 (Invitrogen) and 10 µg BRE-MLP-Luc plasmid was added to the culture flask ...
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bioRxiv - Pathology 2024Quote: ... Isolated EVs were treated with RNAseA (0.02μg/μL) (Invitrogen Cat# 12091021) for 20 minutes at 37°C to degrade extravesicular RNA ...
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bioRxiv - Pathology 2024Quote: ... The relative gene expression levels were determined by quantitative polymerase chain reaction (qPCR) using TaqMan MicroRNA-assays (ThermoFisher) for miR-39 (Assay-ID ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and Nanodrop (Thermo Scientific, Waltham, MA) and sent to Novogene Corporation Inc ...
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bioRxiv - Pathology 2024Quote: ... 20% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin mix (Gibco) ...
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bioRxiv - Pathology 2024Quote: ... 384 well Maxisorp plates (Nunc) were coated with 25 µL 2 µg/mL recombinant human BMP-4 (R&D ...
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bioRxiv - Pathology 2024Quote: ... puromycin (Gibco) was added to the flask for a final concentration of 2 µg/mL to select for cells which had stably incorporated the transfected plasmid ...
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bioRxiv - Pathology 2024Quote: ... for 30min at room temperature after which nuclei were stained with Hoechst (ThermoFisher, dilution 1:10,000) for 10min at room temperature ...
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bioRxiv - Pathology 2024Quote: ... and 1% Penicillin-Streptomycin mix (Gibco)) in a T25 culture flask (Nunc ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA synthesis was conducted using the high-capacity cDNA Reverse Transcription Kit (Applied Biosystems REF 4368814). Analysis of mRNA was performed via quantitative reverse transcription polymerase chain reaction (qRT-PCR) ...
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bioRxiv - Pathology 2024Quote: ... and C-X-C Motif Chemokine Ligand 1 (CXCL1) levels were measured in liver protein lysates and EDTA-plasma by enzyme-linked immunosorbent assay (ELISA) (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... After one last freeze-thaw cycle the total protein content was measured on the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Pathology 2024Quote: ... All real-time qPCR reactions were performed in an AB7000 thermalcycler (Applied Biosystems; CA; USA) using SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Pathology 2024Quote: ... 10 µl of Halt phosphatase inhibitor cocktail (Thermo Scientific) was added ...
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bioRxiv - Pathology 2024Quote: ... 10ng of cDNA template was used to which 1X PowerUp™ SYBR™ Green Master Mix (ThermoFisher) and primers (Eurofins Genomics ...
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bioRxiv - Pathology 2024Quote: ... Equal amount (50 µg) of proteins from each biological sample were reduced by 10 mM TCEP (Thermo Fisher Scientific, Ca# 20490) and alkylated by 20 mM iodoacetamide (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... and alkylated by 20 mM iodoacetamide (Thermo Fisher Scientific, Ca# A39271) in the dark for 45 min at 45 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Leukocytes were then stored in Gibco™ Recovery™ Cell Culture Freezing Medium (12648010, Thermo Fisher Scientific, Zurich, Switzerland).
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bioRxiv - Molecular Biology 2024Quote: ... Liver samples were isolated and stored in RNAlater (Ambion) at −20°C.
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bioRxiv - Molecular Biology 2024Quote: ... followed by Alexa Fluor 568 anti-mouse IgG (Molecular Probes, Invitrogen). Endogenous ERRα was visualized using rabbit anti-ERRα (ab137489 ...
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bioRxiv - Pathology 2024Quote: Total RNA were extracted from the tissue using TRIzol® Reagent according the manufacturer’s instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara) ...
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bioRxiv - Immunology 2024Quote: ... and incubated on ice for 10 minutes and then acquired directly on the Attune NxT flow cytometer (Thermofisher Scientific). 50 µL of cells were acquired per well to enable accurate calculation of cell concentration and wells were resuspended using a multichannel between each column on the 96 well plate to account for cell settling effects.
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bioRxiv - Immunology 2024Quote: ... and Mitotracker Deep Red (MTDR; M22426, Thermofisher Scientific) at 100nM in iron free media for 30 minutes at 37°C/5% CO2 prior to staining ...
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bioRxiv - Immunology 2024Quote: Cells were stained with Mitotracker Green (MTG; M7514, Thermofisher Scientific) and Mitotracker Deep Red (MTDR ...
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bioRxiv - Immunology 2024Quote: ... 50% HBSS (Gibco, 14025-092) and 0.5% BSA (PAN biotech ...
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bioRxiv - Pathology 2024Quote: ... SNARF-5F (S23923, Thermo Fisher Scientific) in a high chloride buffer containing (mM) ...
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bioRxiv - Pathology 2024Quote: ... Tissue Sections were washed three times with PBS and incubated with fluorochrome conjugated secondary antibodies (Thermo Fisher, dilution 1:500) for one hour at RT ...
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bioRxiv - Genomics 2024Quote: ... and 1% glutaMAX (Gibco). Transfection of HEK-293T cells was performed using lipo3000 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... rat anti-mouse CD19 (Invitrogen 13-0194-82), mouse anti-mouse amyloid beta (Clone 6E10 ...
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bioRxiv - Immunology 2024Quote: ... staining and mounting (ProLong Diamond, ThermoFisher, P36965), spleen slides were visualized by microscopy using the Zeiss AxioObserver with Zen Blue (Zeiss ...
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bioRxiv - Genomics 2024Quote: ... The cDNA amplicons were cloned using the TOPO-TA cloning kit and DH5α-T1R Competent Cells (Thermo Fisher Scientific). Plasmid DNA was isolated from bacterial colonies using the EZNA Plasmid DNA Mini Kit I (Omega Bio-Tek Inc.) ...
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bioRxiv - Pathology 2024Quote: ... and cDNA was synthesized from 1 µg mRNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368813). mRNA expression levels were measured with a 7500 Real-time PCR system (Applied Biosystems ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Pathology 2024Quote: ... 1/200 ATG5 (Invitrogen: MA5-35339), 1/2000 GFAP (Invitrogen ...
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bioRxiv - Pathology 2024Quote: ... mRNA expression levels were measured with a 7500 Real-time PCR system (Applied Biosystems) using SYBR green dye (Life Technologies ...
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bioRxiv - Pathology 2024Quote: ... using SYBR green dye (Life Technologies, #4367659). Primer sequences are listed in Table S2.
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bioRxiv - Pathology 2024Quote: ... followed by HBSS digestion buffer (Gibco, #14025-092) containing collagenase D (4.4 U/mouse ...
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bioRxiv - Pathology 2024Quote: ... 10 µl salmon sperm DNA (Invitrogen), 300 ng plasmid DNA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The eluted samples were further concentrated to 1 mL under a gentle stream of nitrogen (40°C, Reacti-vap III, Thermo Scientific) and transferred to HPLC vials ...