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Citations for Thermo Fisher :
4051 - 4100 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... sections were probed with biotinylated monoclonal antibody E492 (3 µg/mL; steamer pH 6.1) alongside a monoclonal antibody either against Clec4F (clone 370901, rat IgG2a, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Rat ES cells derived from SD rats’ blastocyst were cultured on feeder cells with N2B27 plus 2il: Mix DMEM/F12 (Gibco) with Neurobasal medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction started upon the addition of 13 μL of microsome mix from the liver of Sprague-Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... followed by additional 15 min incubation with 10 μg/ml Alexa Fluor 647-conjugated goat anti-rat IgG (cat# A-21247, Invitrogen) or goat anti-mouse IgG (cat # A-21235 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-488 phalloidin (Invitrogen) if needed ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with TBS 3 times and incubated with donkey secondary antibodies (1:500, Alexa-Fluor 488/594/647 anti-rabbit, anti-mouse, or anti-rat, Invitrogen, A21202 ...
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bioRxiv - Immunology 2023Quote: ... and next for 30 min at 37 °C in the presence of chicken anti-rat IgG conjugated with Alexa Fluor 647 (Invitrogen) to stain CD14/IgG on the cell surface and allow its subsequent uptake ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction was started by the addition of 13 μL of microsome mix from the liver of Sprague−Dawley rats (Invitrogen; 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were suspended in a solution of DMEM without phenol red containing 10% rat serum and 1% low-melt agarose (Invitrogen) in a glass capillary tube ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Neuroscience 2023Quote: ... a thin layer of collagen matrix was applied on each coverslip (500µL of 3mg/mL collagen I (from rat tail, Thermo Scientific), 20µL of 1mg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... hBECs were grown in tissue culture flasks and plates pre-coated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium supplemented with 5% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2877597) and Goat anti-Rat IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200, Invitrogen, Cat# A11006, RRID:AB_2534074) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow cells from MycWT/WT or MycT58A/T58A mice were first depleted using purified lineage antibodies described above either using anti-rat IgG Dynabeads (ThermoFisher) or MACS lineage depletion beads on an AutoMACS system (Miltenyi Biotech) ...
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bioRxiv - Immunology 2024Quote: Total IgG from rat serum was purified on drip columns packed with Pierce Protein G agarose resin (Thermo Fisher Scientific). Pierce protein G IgG binding buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... that was manually coated with 50 ug/mL rat tail collagen I (Ref. no. A10483-01, Gibco, Grand Island, NY) prior to cell plating ...
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bioRxiv - Immunology 2024Quote: ... Cells were then isolated as CD4 or CD8 respectively with immunomagnetic beads (Dynabeads Mouse CD4 +, Invitrogen, catalog: 11445D; or Dynabeads Sheep anti-Rat IgG, Invitrogen, catalog ...
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bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
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bioRxiv - Bioengineering 2024Quote: ... the polymerization of EDOT took place on the rat sciatic nerve constructs using ferric chloride (FeCl3) (Thermo Scientific #Cat: 012357.09) oxidant in the presence of ethanol ...
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bioRxiv - Bioengineering 2024Quote: hCMEC/D3 BECs were maintained in tissue culture flasks or multi-well plates pre-coated with 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium comprised of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 raw files were imported into Proteome Discoverer software for library construction using Swiss-iRT and Uniprot-Rat databases (version 2.0, Thermo Scientific). In the sucrose group ...
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bioRxiv - Developmental Biology 2024Quote: ... AlexaFluor555 (anti-mouse) and AlexaFluor488 (anti-rat, both secondary antibodies were from Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) for 45 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 3 hours at RT in secondary antibody (anti-rat AlexaFluor-488, #A21208, or anti-mouse AlexaFluor-488, Invitrogen #A11001 ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used (1:500, goat anti-rat Alexa 488 Invitrogen A11006; 1:500, goat anti-rabbit Alexa 555 Invitrogen A21429 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were diluted in 10% NDS in PBTx and applied overnight: Rabbit anti-Iba1 (1:1,000; Wako; RRID: AB_839504) and Rat anti-GFAP (1:500; Invitrogen; RRID: AB_2532994). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... Remaining cells were washed with PBS and blocked with 10% rat serum and then incubated with following fluorescent monoclonal antibodies from Invitrogen: CD11b eFluor450 (Clone M1/70) ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-mouse IgG (1:300; A21202) or anti-rabbit IgG (1:500; A21206) or anti-rat IgG (1:500; A21208, both Invitrogen), Alexa Fluor 594-labeled donkey anti-goat IgG (1:300 ...
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bioRxiv - Biophysics 2021Quote: ... Cultures were miniprepped using a GeneJET plasmid miniprep kit (Thermo Scientific, cat#K05053). Library completeness was verified by deep sequencing on a MiSeq (Illumina).
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bioRxiv - Biophysics 2021Quote: ... The generated plasmid was then mixed with CellfectinTM II reagent (Thermo Fisher Scientific) in a 1:1 molar ratio and transfected into adherent Sf9 insect cells at a density of 5 × 105 cells per millilitre ...
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bioRxiv - Biophysics 2021Quote: ... The indicated plasmid was transfected into U2OS cells by the Lipofectamine LTX (Invitrogen) according to the standard protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... into the pLX304 plasmid by the Gateway cloning system (Thermo Fisher Scientific, 11791020). For MSI1 overexpression ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient plasmid transfections were performed using Opti-MEM and Lipofectamine 2000 (Life Technologies), and cells were collected for experiments 24 h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... https://plasmid.med.harvard.edu/) The construct was shuttled into the pDEST15 gateway plasmid (Thermo Fisher) carrying an N-terminal GST tag for bacterial protein expression and purification.
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bioRxiv - Cell Biology 2020Quote: ... the p22 expressing plasmids were transfected using Lipofectamin Stem Transfection Reagent (Thermo Fisher). Halotag was labeled by JF635-HaloTag ligand (53) ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng of pFastbac plasmids were transformed into MultiBac DH10 cells (Invitrogen, 10361-012) and positive clones were selected on LB Ampicilin (100μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Expi293F cells were transfected with plasmid DNA using ExpiFectamine transfection reagent (ThermoFisher Scientific). At 72 h post-transfection ...
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bioRxiv - Biophysics 2020Quote: Cells were transiently transfected with plasmid DNA using Lipofectamine 2000 (PN: 11668030, Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were constructed using the GeneArt Seamless Cloning and Assembly Kit (Life Technologies) and propagated in E ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with ASOs and plasmids using Lipofectamine 3000 (Life Technologies, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 microgram of This plasmid was transfected into HeLa cells using cellfectin (Invitrogen) reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... which was cloned into the pCDNA5 FRT/TO plasmid (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with ASOs and plasmids using Lipofectamine 3000 (Life Technologies, L3000015) according to the manufacturer’s protocol ...