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Citations for Thermo Fisher :
4001 - 4050 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and subsequently with Alexa Fluor 647-conjugated donkey anti-rabbit IgG (A31573) or Alexa Fluor 633-conjugated goat anti-rat IgG (A21049) (1:400, Invitrogen) for 3 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000, goat α-rat (AF488, RRID:AB_2534074) at 1:2000, goat α-mouse (AF594, RRID:AB_141372) from Invitrogen (1:2000). Images were quantified using FIJI software (National Institutes of Health ...
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bioRxiv - Neuroscience 2020Quote: ... 1:200 Donkey anti-mouse Alexa Fluor 555 (A-31570) and 1:200 Donkey anti-rat Alexa Fluor 488 (A-21208) were used (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... PP’s were washed 3 times with wash/perm buffer and stained for 30 minutes at RT in Alexa Fluor 546-conjugated anti-Rat IgG (Invitrogen). The PP’s were washed again 3 times with wash/perm buffer and mounted with ProLong Diamond with Dapi mounting solution (Molecular Probes P36962) ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-cleared by incubation with 20μL of IgG from rat serum crosslinked to protein-L Agarose beads (Thermo Scientific) for 2h at 4°C on a rotator ...
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bioRxiv - Immunology 2021Quote: ... secondary antibodies (AF488 donkey anti-rat, AF647 goat-anti rabbit antibody & AF557 donkey anti-mouse antibody, all Thermo Fisher Scientific) were applied in the dark for one hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Following application of Alexa 596 anti-rabbit IgG and Alexa 488 anti-rat IgG (Invitrogen, 1:1000 for 1 hour), cells were fixed in 50:50 acetic acid and ethanol for 10 minutes and DNA was denatured in 2M HCl for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006 ...
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bioRxiv - Developmental Biology 2022Quote: The ten raw files were searched against the Swiss-Prot rat database with SEQUEST HT by Proteome Discoverer (version 2.1; Thermo Scientific). A maximum of two missed cleavage sites were allowed in the trypsin digestion ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: mLECs were isolated from collagenase I-digested lung tissue using rat anti-mouse CD31 monoclonal antibody-coated Dynabeads (11035, Invitrogen) and directly used for RNA analysis.
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bioRxiv - Cell Biology 2022Quote: ... embryos were incubated for two hours at room temperature with fluorescently-labelled secondary antibodies diluted 1:100 in BBT (Alexa 488-conjugated goat anti-rabbit IgG, Alexa 555-conjugated goat anti-rat IgG, Alexa 647-conjugated goat anti-mouse IgG; Invitrogen). After five final washes in PBT ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in 1 mL of the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000 for Al1 in pupae and 1:3000 for Al1 in embryos, Donkey anti-rat-AlexaFlour 555 [Thermofisher] at 1:1000 for Al2 in Pupae and 1:3000 for Al2 in embryos and Hoechst 33342 at 1:1000 [Thermofisher] in blocking buffer) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 1:100) followed by anti-rat secondary antibody conjugated with Alexa Fluor 647 (Thermo Fisher, catalog no. A21247, 1:200). Subcapsular sinus macrophages were labeled using anti-sialoadhesin (CD169 ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 90 min at 37 °C with AlexaFluor-488 conjugated goat anti-mouse and AlexaFluor-568 goat anti-rat antibodies and DAPI (Invitrogen). Fluorescence microscopy was performed using a Nikon Structured illumination microscope (N-SIM ...
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bioRxiv - Microbiology 2022Quote: ... then incubated with secondary IgG (goat anti-mouse Alexa546, goat anti-rabbit Alexa633, goat anti-rat, all 1:500, ThermoFisher) in PBS containing 0.5% Triton X-100 for 2 hours and counterstained with Hoechst 33342 (1 μg ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from the thalamus of 2 GAERS (10 and 90 days) and 2 NEC (10 and 90 days) male rats using a MagMax Kit (Ambion) and full length cDNAs generated using SuperScriptII with oligo-dT priming (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Rat embryonic primary hippocampal cell cultures were prepared and transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Invitrogen, catalog no. 11668019) as described previously (Janusz et al. ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cortical astrocytes were dissected from SD neonatal rats (1-3 days) with the aseptic operation and digested by 0.25% trypsin (Gibco, USA) at 37°C for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Rat: anti-SOX2 (Thermo, 14-9811-82 1:500) Other: Streptavidin-Alexa Fluor 594 conjugate (Thermo Scientific, S11227, 1:500).
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bioRxiv - Bioengineering 2022Quote: ... or transwell inserts precoated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Neuroscience 2024Quote: ... used for nNOS immunolabeling, and 568-conjugated donkey anti-rat secondary antibody (1:500; Cat#?, Lot#) used for GnRH were purchased from Invitrogen.
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bioRxiv - Neuroscience 2024Quote: ... sections were washed with TBS 3 times and incubated with donkey secondary antibodies (1:500, Alexa-Fluor 488/594/647 anti-rabbit, anti-mouse, or anti-rat, Invitrogen, A21202 ...
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bioRxiv - Immunology 2024Quote: Total IgG from rat serum was purified on drip columns packed with Pierce Protein G agarose resin (Thermo Fisher Scientific). Pierce protein G IgG binding buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2877597) and Goat anti-Rat IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200, Invitrogen, Cat# A11006, RRID:AB_2534074) were used ...
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bioRxiv - Neuroscience 2024Quote: ... mice and rats were heavily anesthetized with ∼1.0-2.0 ml of urethane (Fisher Scientific, CA#AC325540500, 30% w/v, i.p.) and transcardially perfused with 120 ml of 1% PB followed by 180 ml of 4% granular PFA in PB ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow cells from MycWT/WT or MycT58A/T58A mice were first depleted using purified lineage antibodies described above either using anti-rat IgG Dynabeads (ThermoFisher) or MACS lineage depletion beads on an AutoMACS system (Miltenyi Biotech) ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were 1:5000 anti-rat Alexa488 and anti-mouse Alexa594 conjugated antibodies (Cat #A32740 Invitrogen, CA, USA). The sections were mounted using ProLong Gold with DAPI (Cat #S36964 Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 45[min at room temperature with the following secondary antibodies: CD41 was visualized with the antibody goat anti rat Alexa Fluor 488 (Catalog #A-11006, Invitrogen); the LSP1 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies (rabbit anti–GFP, 1:1,000 dilution; rat anti–mCD8, 1:200 dilution, ThermoFisher Scientific) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 FITC-conjugated, 1:200, Invitrogen) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Genetics 2023Quote: The brains of E13.5 rats were lysed and 30 μg of protein combined with 5 μl 4x LDS Sample Buffer (ThermoFisher, #NP0004) and 2 μl 10x Sample Reducing Agent (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... hBECs were grown in tissue culture flasks and plates pre-coated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium supplemented with 5% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2023Quote: ... the samples were again incubated overnight with secondary antibodies (ThermoFisher, anti-rat polyclonal conjugated with Alexa 594, 1:300; ThermoFisher anti-rabbit polyclonal conjugated with STAR RED 1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices from embryonic day 18 Sprague Dawley rat were dissociated enzymatically in trypsin supplemented with 0.25% EDTA (Thermo Fisher Scientific) and physically by trituration ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-mouse or anti-rat or anti-rabbit fluorescein-conjugated secondary antibodies (dilution 1:500, Invitrogen) and cell nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rat Alexa 488 and goat anti-mouse IgM Alexa 555 (dilution 1:250; all secondary antibodies were obtained from Invitrogen). Specificity of the secondary antibody was verified by including a control staining in which the primary antibody was omitted (data not shown) ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were stained for 1 hour at room temperature with anti-rat secondary antibody (Alexa Fluor 488; Thermo Fisher Scientific) followed by 2 minutes of Sudan Black (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... No: A36511) were thawed and co-cultured with 15% primary rat cortical astrocytes (Cat. No: N7745100) both obtained from ThermoFisher Scientific (US) ...
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bioRxiv - Neuroscience 2023Quote: ... The following siRNAs were used for knockdown of adult rat-derived hippocampal NSCs: Stealth RNAi™ siRNA Derl1-MSS289837 (Invitrogen), Stealth RNAi™ siRNA Stat5b-RSS332572 (Invitrogen) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded at the final density of 2×106 cells/ml in the central chamber of the DAX-1 chips embedded in a matrix composed of type I rat tail collagen at the final concentration of 2.3 mg/ml (Thermofisher, #A1048301). Each chip can be loaded with 10µl of gel ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were washed three times with PBS + 0.05 % Tween 20 and stained with anti-rat AF564 (1:100, Invitrogen, A11007) and SYTOX™ Green (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...