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Citations for Thermo Fisher :
4001 - 4050 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The eluting peptides were ionized at a potential of + 2 kVa into a Q Exactive mass spectrometer (Thermo Fisher, Germany). Intact masses were measured at resolution 70.000 (at m/z 200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
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bioRxiv - Biochemistry 2023Quote: ... We analyzed MS/MS data collected from ND samples to identify the likely sequence of the patent peptides using SEQUEST (Bioworks v3.3.1, Thermo Fisher Scientific) with a peptide tolerance of 8 ppm and a fragment tolerance of 0.1 AMU ...
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bioRxiv - Cell Biology 2024Quote: ... The digested peptides were then desalted using Pierce C18 Spin Tips & Columns system according to the manufacturer’s instructions (ThermoFisher Scientific). Three independent biological replicates for each condition were analyzed by mass spectrometry.
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bioRxiv - Neuroscience 2024Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: Enriched crosslinked peptides were injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific™) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on a EASYspray C18 column (50 cm x 75 um, Thermo Fisher Scientific, San Jose, CA) over a 65-minute gradient at a flow rate of 250 nL/min ...
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bioRxiv - Cancer Biology 2024Quote: T cells from KOG mouse TDLNs (5 × 105) were co-cultured with SIINFEKL peptide (1 μg/mL, Thermo Fisher Scientific) in a 96-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 75 µm ID x 25 cm C18 Acclaim PepMap® RSLC column (Thermo Scientific, USA) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
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bioRxiv - Biochemistry 2024Quote: The tryptic peptides were injected onto a pre-column (PepMap C18, 5 mm × 300 μm × 5 μm, Thermo Fisher Scientific) and separated ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
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bioRxiv - Biochemistry 2024Quote: ... and the resulting peptides were analysed by LC-MSMS on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) using CID+ETD activation ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific) per channel ...
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bioRxiv - Molecular Biology 2024Quote: The resulting peptide mixtures were analyzed in data-dependent acquisition mode with an Orbitrap Exploris high-resolution mass spectrometer (MS) (ThermoFisher) coupled to an UltiMate 3000 LC system (Dionex-LC Packings) ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were first loaded onto an Acclaim PepMap C18 nano-trap column (100Å, 3µm, 75µm x 2cm, Thermo Scientific 164946) in mobile phase A ...
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bioRxiv - Physiology 2024Quote: ... and 10 μg of peptide per sample was labeled with TMTpro18 plex reagents (Cat. # A52045; Thermo Fisher Scientific, Darmstadt, Germany). TMT-labeled samples were adjusted to equal amounts and pooled ...
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bioRxiv - Cancer Biology 2024Quote: The peptide samples (1-1.5 μL) were analyzed with a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray source operating at 2 kV at a capillary temperature of 320 °C and coupled to an EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 4 µg of peptides were analyzed in a QExactive mass spectrometer coupled to a nano EasyLC 1000 (Thermo Fisher Scientific). Solvent composition at the two channels was 0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated for 1 hour at room temperature in blocking buffer containing the following secondary antibodies: Goat anti-Rabbit Alexa Fluor® 594 conjugate (1:250, #A-11037, Life Technologies) and Donkey anti-Mouse Alexa Fluor® 488 conjugate (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were permeabilized with 0.5% Triton X-100 in DPBS for 10 minutes and incubated in BlockAid Blocking Solution (Thermo Fisher, Cat# B10710) for 1 h at RT in a humidity chamber and incubated O/N in primary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... NRK cells expressing WT importin α or mutant importin α S62A released from thymidine blocking were changed to CO2-independent medium (Invitrogen), 10% cosmic calf serum (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections of the cochlea and brain slices were washed in PBS for 1 h and subsequently incubated in secondary antibody solution containing blocking solution and the secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit (1:1000, cochlear cryosections) (Molecular Probes (A11008)) ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were then washed three times with 1xPBS and incubated with Alexa 594 conjugated anti-mouse IgG antibody (1:500 in blocking buffer, ThermoFisher Scientific, A11012) and Alexa 488 conjugated anti-rabbit IgG antibody (1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for one hour on the rocker at room temperature in blocking solution with 1:1000 Goat anti-Rabbit IgG H&L Alexa Fluor 405 (Thermo Fisher Scientific). Then sections were washed once in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3×5mins in PBS and incubated for two hours in appropriate secondary antibodies (Invitrogen; 1:250 in blocking solution). For double labelling of Iba1 and GAD67 ...
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bioRxiv - Bioengineering 2019Quote: ... cardiomyocytes were incubated in blocking solution containing Alexa Fluor Plus 488-conjugated goat anti-mouse IgG (1:300, A32723; Thermo Fisher Scientific) for 60 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were rinsed three times with PBS and blocking buffer with AlexaFluor 488-coupled goat anti-mouse antibody (Life Technologies, 1:1000) and 0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... After rinsing in PBS and incubating in blocking buffer (10% normal goat serum, Cat #PCN5000 and 0.2% Triton™ X-100 Cat #9002-93-1, ThermoFisher, in PBS) for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 x 10 min in PBS and incubated in goat anti-rabbit Alexa Fluor 647 (1:500 in blocking buffer; catalog no. A32733, Invitrogen, Carlsbad, CA) for 2 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Molecular Biology 2021Quote: ... coverslips were washed 3 times in PBS + 0.1% Tween then incubated at room temperature in the dark for 1 hour in blocking buffer containing Alexa Fluor 546 goat anti-rabbit IgG (Invitrogen, A-11035) secondary antibody (1:1500 ...
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bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% PFA in phosphate-buffered saline (PBS) for 30 min and blocked with blocking buffer consisting of 10% horse serum (Life Technologies, 26050088), 0.1% gelatin ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed twice with PBS and incubated in the blocking buffer containing donkey anti-goat IgG antibodies conjugated with Alexa Fluor 568 (ThermoFisher Scientific, USA) diluted 1:400 for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3×10 mins in IF blocking buffer prior to mounting on to coverslides with ProLong Diamond Antifade Mountant with DAPI (ThermoFisher Scientific #P36962). Slides were allowed to cure overnight before imaging with either a Zeiss AxioObserver widefield microscope (20x ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 x 5m in PBS and then incubated 1h at RT with the following secondary antibodies all diluted 1:1000 in blocking solution: donkey anti-rabbit Alexa Fluor 647 (ThermoFisher A-31573); goat anti-mouse Alexa Fluor 568 (ThermoFisher A-11031) ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary labelling was done for 1 hour in blocking buffer containing Alexa Fluor 488 goat anti-rabbit IgG (InVitrogen, Waltham, MA (A11008)) or Alexa Fluor 488 goat anti-rat (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μg/mL Hoechst 33342 (Fischer Scientific BDB561908) in the donkey blocking solution before washing and mounting on slides with Immu-Mount (Thermo Fisher Scientific) and 1.5 mm coverslips (EMS).
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bioRxiv - Immunology 2022Quote: ... The next day the plates were washed three times with TBS and blocked for 30 min with Casein blocking buffer (Thermo Fisher Scientific). After washing the plates with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were kept in the dark in blocking solution containing donkey anti-rabbit Alexa Fluor ® 647 (1:500 ab150075 Invitrogen®) and Goat anti-mouse Alexa Fluor ® 488 (1:500 A11001 ThermoFisher® ...