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Citations for Thermo Fisher :
4101 - 4150 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were subsequently washed with PBS and incubated with fluorophore-conjugated secondary antibodies in blocking solution [anti-rabbit IgG Alexa Fluor™ 594 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of Brilliant Stain Buffer Plus (10 μl/sample) (cat# 563795, Becton Dickinson, Mountain View, Ca, USA) and CellBlox Blocking Buffer (5 μl/sample) (cat# C001T02F01, Thermo Fisher Scientific). Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401 ...
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bioRxiv - Synthetic Biology 2024Quote: ... All antibodies were diluted in blocking buffer as described in the Bioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00).
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:300 in the same blocking solution as well as the secondary antibody Alexa Fluor 647 (Cat. No. A21244, Life Technologies, USA). Plasma membranes were stained with wheat germ agglutinin (WGA ...
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bioRxiv - Genomics 2024Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific], Donkey anti-mouse Alexa Fluor 488 [A21202; Fisher Scientific] ...
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 hour at room temperature and subsequently incubated in the blocking buffer containing rabbit anti-SYNGAP1 (1:5000; ThermoFisher #PA1-046), rabbit anti-SRGAP2A (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then exposed at room temperature first for 1 hour to primary Abs in blocking buffer and then for 30 minutes to Alexa Fluor–conjugated secondary Abs (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed in PBS for 5 minutes and subsequently incubated in blocking solution for 15 minutes (Pierce Super Blocker; Thermo Fisher Scientific). The slides were incubated in primary antibody solution (1x PBS ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 h at room temperature preceding primary antibody incubation using a 1:1 mixture of Odyssey Blocking Buffer and TBST (ThermoFisher, J77500.K8) diluted to 1x concentration at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
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bioRxiv - Neuroscience 2024Quote: The slides were pre-incubated in blocking solution and stained against Griffonia simplicifolia isolectin GS-IB4 Alexa 568 (Invitrogen, I21412; 1:500), Neurofilament 200 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... The hydrated slide is blocked in 3% skim milk PBST for 1 hour and incubated with primary antibody (1:100) diluted in Superblock Blocking Buffer in TBS (#37535, Thermo Fisher Scientific) at room temperature overnight ...
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bioRxiv - Plant Biology 2019Quote: ... Two μL (~1μg) peptides were analyzed by LC-MS with a Dionex RSLCnano HPLC coupled to a LTQ-Orbitrap Velos Pro (Thermo Scientific) mass spectrometer using a 2 h gradient ...
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bioRxiv - Biophysics 2021Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were separated on an EASY-Spray™ Acclaim PepMap C18 column (50 cm × 75 μm inner diameter, Thermo Fisher Scientific) using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptides were analyzed on a LC-MS/MS platform consisting of an Easy-nLC 1200 UHPLC system (Thermo Fisher Scientific) interfaced with an QExactive HF orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the extracted peptides were completely dried to completion and thereafter desalted using the SPE Pierce C18 Spin Columns (Thermo Fisher Scientific). These columns were activated by 2 × 200 μL of 50% acetonitrile (CAN ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were passed through an immobilized pepsin column (2 mm□× 2 cm) at 120 μL min−1 and the digested peptides were captured on a C18 PepMap300 trap column (Thermo Fisher) and desalted ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described (36).
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bioRxiv - Molecular Biology 2022Quote: ... Target peptides for each of these proteins were purchased in stable isotope labelled synthetic peptides (SIS) form in crude quality from Thermo Scientific. The C-terminal lysine or arginine in the SIS peptides were replaced by isotope labelled lysine (13C6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
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bioRxiv - Cell Biology 2022Quote: Fractions of crosslinked peptides were analyzed by liquid chromatography coupled to tandem mass spectrometry using an LTQ Orbitrap Elite (Thermo Scientific) instrument 65 ...
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bioRxiv - Biochemistry 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Immunology 2019Quote: ... and resulting peptides were fractionated and sequenced by nanoflow LC-electrospray MS/MS on an Orbitrap Velos Pro hybrid mass spectrometer (Thermo Scientific), in the UT Austin mass spectrometry core facility as described previously (26) ...
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bioRxiv - Molecular Biology 2020Quote: ... High pH reversed fractionation was achieved by attaching a XBridge Peptide BEH C18 column (Cat. 186003570; Waters Corp.) to an Accela liquid chromatography system (Thermo Scientific) using the HPLC application ...
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bioRxiv - Molecular Biology 2020Quote: ... The column was enclosed into a column oven (Sonation) and the peptides were sprayed into a Q Exactive Plus mass spectrometer (Thermo Fisher). The mass spectrometer was operated in a data-dependent acquisition mode using a top10 method ...
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bioRxiv - Immunology 2022Quote: The encapsulation efficiency of the peptide into the NLC formulation was determined via the CBQCA protein quantitation kit (Thermo Fisher Scientific). Three separate NLC peptide formulations were created using the optimized formula previously determined in the DOE ...
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bioRxiv - Genomics 2020Quote: ... Flag peptide and small size of non-specific proteins were removed by 20K Slide-A-Lyzer dialysis cassette (ThermoFisher Scientific, 66003) with 1L binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to Sequelon-AA membrane (Applied Biosystems) as described previously [40].
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bioRxiv - Immunology 2019Quote: ... prior to injection of 2 µg of purified peptides into an Ultimate3000 nano-LC system coupled to a Q Exactive mass spectrometer (ThermoFisher Scientific) for mass spectrometry ...
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bioRxiv - Genetics 2020Quote: ... Peptide mixtures were analysed by nLC-ESI-MS/MS on a LTQ-Orbitrap XL coupled to an Easy nLC (Thermo Scientific). The sample preparation and the nLC-ESI-MS/MS analysis were performed as previously described (Rappsilber et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by MS measurement of peptides and their fragmentation spectra (LC-MS/MS) with a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2019Quote: The peptide samples resulting from the above digestion were separated by an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, USA). First ...
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bioRxiv - Plant Biology 2019Quote: The separated peptides were analyzed on-line by using a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operated in standard data-dependent acquisition mode controlled by Xcalibur version 4.0.27.19 ...
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bioRxiv - Synthetic Biology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1575 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
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bioRxiv - Cell Biology 2019Quote: ... in accordance with the manufacturer’s instructions and then fractionated using a Pierce high-pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Eight fractions were collected ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Peptides were separated on a column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, Thermo Fisher Scientific). The column had a 100 μm inner diameter microcapillary ...
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bioRxiv - Molecular Biology 2019Quote: Peptide fractions from the small-scale high pH reversed phase separation were analysed on an Orbitrap Tribrid Fusion (Thermo Fisher Scientific) with the same MS parameters as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...