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Citations for Thermo Fisher :
3851 - 3900 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... with or without the presence of peptides or anti-CD3 and inhibitors in a final volume of 200 μl RPMI1640 + L-glutamine (Gibco) containing 10 % FCS and penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Protein samples were injected for inline pepsin digestion and the resulting peptides were identified using tandem MS (MS/MS) with an Orbitrap mass spectrometer (Fusion Lumos, ThermoFisher). Following digestion ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 μg peptides from each time course was labeled with iTRAQ™ (Applied Biosystems, Foster City, CA) as described by the manufacturer (0 hour/control ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides were directly eluted (∼250nl/min) via a Triversa Nanomate nanospray source into a Orbitrap Fusion mass spectrometer (Thermo Scientific). Positive ion survey scans of peptide precursors from 375 to 1575 m/z were performed at 120 K resolution (at 200m/z ...
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bioRxiv - Cell Biology 2020Quote: ... peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 2.5 kV distal voltage electrosprayed the eluting peptides directly into LTQ linear ion trap mass spectrometers (Thermo Scientific) equipped with a custom-made nano-LC electrospray ionization source ...
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bioRxiv - Cancer Biology 2021Quote: ... The redissolved peptides of each sample were acquired and analyzed by EASY-nLC™ 1200 System (Thermo Fisher Scientific™) coupled to a QE HF-X mass spectrometer (Thermo Fisher Scientific™) ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were first trapped onto a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide extracted from each set were pooled and analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Thermo Scientific) coupled to a TripleTOFTM 6600 mass spectrometer (AB Sciex) ...
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bioRxiv - Biochemistry 2021Quote: LC-MS analyses of tryptic peptide mixtures were performed using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) connected to an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Synthetic peptides (60 nmol each) were incubated with 18 μL streptavidin agarose resins (high capacity streptavidin agarose, Thermo Fisher Scientific) per experiment for 2 hr at room temperature in 80 μL of lysis buffer (1% NP-40 ...
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bioRxiv - Biochemistry 2021Quote: ... Eluting peptides were electrosprayed into the mass spectrometers (Exactive Plus EMR-Orbritrap or Q-exactive HF, both Thermo Fisher Scientific). Data were recorded using Xcalibur software (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... peptides were reconstituted in 6 μL of loading buffer (2% ACN, 0.1% TFA) and peptide concentration was estimated via optical measurement at 280 nm (Nanodrop 2000; Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Biochemistry 2020Quote: ... 1 nM of each acyl-peptide was added to 100 ng of a HeLa tryptic digest standard (Thermo Scientific, #PI88329) for a final ratio of 1 fmol acyl-peptide/100 ng of HeLa digest ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50-cm by 75-μm ID; ThermoFisher Scientific). The 125-min elution gradient at a constant flow rate of 300 nl/min was set to 5% phase B (0.1% formic acid in 99.9% acetonitrile ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were eluted from the column and electrosprayed into a Velos Pro Linear Ion Trap Mass Spectrometer (Thermo Fisher Scientific). Data was acquired using data-dependent acquisition (DDA ...
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bioRxiv - Immunology 2020Quote: ... Peptide stimulated and non-stimulated cells were first labeled with live/dead detection kit (Thermo Fisher Scientific, Waltham, MA, USA) and then resuspended in BD Fc Block (clone 2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of peptides was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Developmental Biology 2021Quote: ... The digested peptides were eluted on a capillary column and sprayed into a Q Exactive Mass Spectrometer (Thermo Fisher Scientific) equipped with a nano-ESI ion source (National institute of Bilogical Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated into eight fractions using the High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific Inc.). The fractions were dissolved in 0.1% formic acid ...
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bioRxiv - Immunology 2022Quote: The tryptic peptides were loaded onto an Acclaim PepMap 100 C18 trapping column(C18, 1.9 μm, 75 μm*20 cm; Thermo Fisher) at a flow rate of 200 nL/min ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS: Generated peptides were injected into a PepMap C18 5 mm x 300 um column (Thermo Fisher Scientific) at a flow of 25 μL/min with 0.1% TFA mobile phase ...
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bioRxiv - Cell Biology 2022Quote: ... LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Fisher Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 200ng of tryptic peptides were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using Ultimate 3000 UHPLC (ThermoFisher) connected to an Orbitrap Fusion Lumos Tribrid (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The dried peptides were resuspended in 200 mM EPPS pH 8.5 and quantified by bicinchoninic acid assay using the quantitative colorimetric peptide assay from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Digested peptides were analyzed by Orbitrap Fusion Lumos mass spectrometer coupled with EASY-nLC™ 1200 system (Thermo Fisher Scientific) for PRM ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Cross-linked peptides were analyzed by an Easy nanoLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) using data-dependent acquisition of ions with a charge state between 3 and 8 and HCD fragmentation using stepped normalized collision energy (NCE ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were first separated by an Ultimate 3000 RSLC nano and a Q-Exactive Plus mass spectrometer (both Thermo Scientific). Settings were set as described previously (https://pubmed.ncbi.nlm.nih.gov/30638812/) ...
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bioRxiv - Biochemistry 2022Quote: Peptides were resuspended in 0.5% formic acid and analysed using nanoflow LC-MS/MS using an Orbitrap Elite (Thermo Fisher) hybrid mass spectrometer equipped with a nanospray source ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were loaded onto a reversed phase trap column (Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Scientific) connected to the C18-reversed phase analytical column (EASY-Column ...
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bioRxiv - Microbiology 2022Quote: ... The peptide eluent from the C18 column was electrosprayed in Thermo Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, USA) in positive ion mode using 2.2 kV voltage ...
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bioRxiv - Immunology 2022Quote: ... Peptides were separated by reverse phase chromatography using a C18 pre-column (Acclaim PepMap 100, 75μm×2cm, Thermo Fisher Scientific) followed by C18 reversed-phase LC column (PepMap RSLC C18 ...
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bioRxiv - Immunology 2022Quote: ... 106 splenocytes were ex vivo stimulated with SARS-CoV-2 peptide pool (JPT PM-WCPV-S-1) at concentration of 2 μg/ml of each peptide in RPMI (Biowest) supplemented with 10% heat inactivated FBS (Gibco). PMA/Ionomycin (BD Pharmingen™ ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were first trapped onto a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with Buffer A at a flow rate of 2.5 µL/min over 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... fractions containing tryptic peptides were resolved and ionized using nanoflow UHPLC (Easy-nLC 1200 system, Thermo Fisher Scientific Inc., USA) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: ... The peptides were fractionated using high-pH HPLC methods and were then analyzed in Orbitrap Fusion™ Mass Spectrometer (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... Peptide analyses (corresponding to 1 µg protein) were performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) connected to an EASY-nLC 1200 ultra-HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... VTWFHAIHVSGTNGT31) immunodominant spike peptides (1 μg/mL) overnight at 37°C in the presence of Brefeldin A (1:500, Invitrogen). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on a 250 mm x 75 μm Acclaim PepMap® C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...