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Citations for Thermo Fisher :
3801 - 3850 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Biophysics 2022Quote: The GyrA14-E487C and the CcdA50-72 peptide (Table S1) were labeled with Fluorescein-5-Maleimide and 5-TAMRA (Tetramethylrhodamine-5-Maleimide) from ThermoFisher Scientific as per the manufacturer’s protocol as described previously (Aghera et al. ...
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bioRxiv - Microbiology 2022Quote: ... the LC-MS/MS data were used as the input for peptide identification using the database search engine SEQUEST HT implemented in Proteome Discoverer 2.2 (Thermo Scientific). Note ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatographic separation of the peptides was performed by liquid chromatography on a nano-UHPLC system (Ultimate UHPLC focused, Thermo Scientific) using a nano column Acclaim PepMapC100 C18 ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Fractionated peptides were ionized and analyzed by tandem mass spectrometry (MS/MS) Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Label-free quantitation was performed using the MaxQuant software package.79 The EMBL Human reference proteome (UP000005640 9606 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded first onto an Acclaim PepMap 100 Trapping column (Thermo Scientific, 20 mm 75 µm inner diameter (ID), 3 µm of C18 resin with 100 Å pore size ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli digests and Promega yeast protein digest were each fractionated by offline reversed-phase LC (Waters Acquity UPLC Peptide BEH300 C18 1.7 µm, 1 mm i.d. x 150 mm column employing an Ultimate 3000 HPLC, Thermo Fisher Scientific) operating with column compartment temperature of 60 °C and flow rate of 100 µL/min ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was affinity purified on phospho-peptide covalently bound to a SulfoLink column according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Peptide fractions were analysed in a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) coupled on-line with a nanoflow ultrahigh-performance liquid chromatography (nano-UPLC ...
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bioRxiv - Neuroscience 2020Quote: ... Per measurement 0.5 μg of peptides were automatically loaded to the online coupled RSLC (Ultimate 3000, Thermo Fisher Scientific Inc.) HPLC system ...
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bioRxiv - Bioengineering 2021Quote: Around 1 µg of peptides were run on the pre-column (Acclaim PepMap™ 100, 75μm × 2cm, nanoViper, Thermo Scientific) and the analytical column (Acclaim PepMap™ RSCL ...
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bioRxiv - Biochemistry 2021Quote: ... The application of a 2.5 kV distal voltage electrosprayed the eluting peptides directly into LTQ linear ion trap mass spectrometers (Thermo Scientific) equipped with a custom-made nano-LC electrospray ionization source ...
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bioRxiv - Biochemistry 2020Quote: Both NPC1 and synthetic peptide samples were analyzed by LC-MS using a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex Ultimate 3000 LC-system (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... was constructed by cloning the 2A peptide-linked Cas9 ORF from the GFP_2A_Cas9 expression vector46 into a pcDNA™3.1(+) vector (Thermo Fisher Scientific) between the HindIII and BamHI sites ...
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bioRxiv - Neuroscience 2022Quote: ... All peptide samples were analyzed on an Orbitrap Fusion Lumos Tribrid Mass Spectrometer with an Ultimate3000 RSLCnano system (Thermo Scientific). They were loaded C18 particles at 8 μL/minute flow rate ...
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bioRxiv - Developmental Biology 2022Quote: ... Analyses of peptide digests were carried out on an Orbitrap Q Exactive HF-X instrument (Thermo Fisher Scientific, Bremen, Germany). The instrument was configured for data-dependent methods using the full MS/DD?MS/MS setup in a positive mode ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of each peptide extracts were loaded in two or three injection replicates onto 300μm ID x 5mm PepMap C18 precolumn (ThermoFisher, Dionex) at 20 μl/min in 2% acetonitrile ...
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bioRxiv - Immunology 2022Quote: For LC-MS analysis of wild type ILC2 1.5 µg of peptide for each sample was analysed on a Q-Exactive-HF-X (Thermo Scientific) mass spectrometer coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Molecular Biology 2019Quote: ... TAILS and secretome peptide mixtures were loaded onto an PepMap100 C18 precolumn (75 μm x 2 cm, 3μm, 100Å, nanoViper, Thermo Scientific) and separated on a PepMap RSLC C18 analytical column (75 μm x 50 cm ...
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bioRxiv - Systems Biology 2019Quote: ... The cross-linked peptides were then enriched by HILIC on the Dionex UltiMate 3000 Series HPLC instrument (Thermo Fisher Scientific) equipped with a TSKgel Amide-80 column (3 µm ...
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bioRxiv - Systems Biology 2019Quote: ... Labelled peptides from each set of replicates were then combined and cleaned up using SCX cartridge system (Applied Biosystems, USA) prior to 2D-LC-MS analysis.
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bioRxiv - Cancer Biology 2019Quote: The peptides were analysed on a Thermo Scientific™Orbitrap Fusion™ Tribrid™ Mass Spectrometer (Thermo Scientific, Bremen, Germany) interfaced with Easy-nLC 1000II nanoflow liquid chromatography system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (ThermoFisher), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Microbiology 2019Quote: ... Peptides mixtures were analyzed by a Q-Exactive Plus coupled to a Nano-LC Proxeon 1000 (both from Thermo Scientific). Peptides were separated by chromatography with the following parameters ...
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bioRxiv - Immunology 2020Quote: ... Reconstituted peptides were tested for endotoxin contamination using a Limulus Amebocyte Lysate Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, UK #88282).
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bioRxiv - Cell Biology 2019Quote: Tryptic peptides were analyzed using an EASY-nLC 1000 coupled to a Q ExactiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-MreBCT antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), rabbit anti-Hsp60 (kind gift of Dr ...
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bioRxiv - Microbiology 2020Quote: The tryptic eluted peptides were dried by speed-vacuum centrifugation and then desalted onto StageTip C18 Pipette tips (Thermo Scientific) until the mass spectrometric analysis ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were dissolved in 3% acetonitrile/0.1% formic acid and 200 ng of peptides were injected on an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC (Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Plant Biology 2021Quote: 100 μg peptide mixture of each sample was labelled using TMT reagent according to the manufacturer’s instructions (Thermo Fisher Scientific) and analysed on an Orbitrap Fusion Lumos (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 µg peptides from each sample were labeled using the TMT11plex Mass Tag Label Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were eluted and ionized by electrospray followed by mass spectrometric analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). MS data were acquired using the FTMS analyzer and MS/MS data using the ion trap analyzer ...
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bioRxiv - Biophysics 2019Quote: ... Cleavage of the signal peptide was confirmed by transfecting and expressing individual constructs and ensuring they were secreted from transfected Expi293 cells (ThermoFisher) and by glycosylation-corrected molecular weight determination using SEC-MALS (Wyatt Technology).
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bioRxiv - Neuroscience 2019Quote: ... RAI1 antibodies were generated by immunizing rabbits with a synthesized RAI1 peptide (aa 28 to 42, ENYRQPGQAGLSCDR, Thermo Fisher Scientific), followed by affinity purification using the peptide as the affinity ligand (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... the peptides were separated by reversed-phase high-performance liquid chromatography using an Easy-nLC 1000 Nanoliquid chromatography (ThermoFisher Scientific). The separation was carried out in a homemade column 25 cm in length and 100 μm in diameter packed with a C18 adsorbent ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... In all cases peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Neuroscience 2020Quote: ... we designed a QuasAr-P2A-GCaMP/QuasAr-P2A-vGlut-pHluorin by inserting QuasAr fused to P2A peptide synthesized using GeneArt Gene Synthesis (Invitrogen) and GCaMP/vGlut-pHluorin under the human Synapsin1 promoter ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were eluted with 0.1% (v/v) trifluoroacetic acid (TFA) and dried in a SpeedVac system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Scientific) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm × 25 cm reverse-phase NanoEase M/Z HSSC18 T3 column (Waters) ...