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Citations for Thermo Fisher :
3951 - 4000 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: LC-MS/MS analysis of TMT-labeled peptides was conducted on an Orbitrap Fusion Lumos tribrid mass spectrometer (Thermo Scientific) coupled with an Easy-nLC 1200 UPLC system (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: The raw LC-MS/MS data was analyzed against the Uniprot GaHV2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a PepmapTM C18 reversed phase column (3 μm, 100 Å, 75 μm × 50 cm) (Thermo Scientific). Samples were fractionated with mobile phase A consisting of 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted and ionized by electrospray followed by mass spectrometric analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). MS data were acquired using the FTMS analyzer and MS/MS data using the ion trap analyzer ...
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bioRxiv - Microbiology 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Fisher Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Each peptide extract was loaded on a 300 µm ID x 5-mm PepMap C18 pre-column (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted and separated on an C18 Acclaim PepMap RSLC analytical column (75 µm x 250mm, Thermo Fisher Scientific) with a flow rate of 300 nl/min in a 120 min gradient of 3% buffer A (0.1% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 200 ng peptide material was measured on a Q Exactive HF-X Orbitrap mass spectrometer (Thermo Fisher), coupled to the LC-system with a nano-spray ion-source using coated emitter tips (PepSep ...
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bioRxiv - Biochemistry 2023Quote: ... The digestion was quenched in 0.5% formic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed and then treated with peptide in NP-40 and Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher) for 10 minutes at room temperature while rocking ...
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bioRxiv - Genetics 2023Quote: ... Bin3-3xHA complexes were purified by two sequential competitive elutions with 75 µl of Pierce HA Peptide (Thermo Scientific, 26184) diluted to 2 mg/ml in Nuclease-Free Water (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Plant Biology 2023Quote: Peptide/Protein identification and MS1-based label-free quantification were carried out using Proteome Discoverer 2.5 (PD2.5) (Thermo Fisher Scientific) or Skyline version 22.2 (MacCoss lab software) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteolytic peptides were finally concentrated in a trapping column (C18 Pepmap, 300 μm i.d. × 5 mm length, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Non-deuterated samples peptide identification was performed via data dependent tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.3 (Sierra Analytics ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped in a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2/98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Genetics 2023Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific, Waltham, MA) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: TMT-labeled peptides were analyzed using an EASY-nLC 1200 UPLC coupled with an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). LC buffer A (0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... MS data of the peptides were acquired on a Q Exactive mass spectrometer coupled to EASY-nLC (Thermo Fisher Scientific) using a data-dependent top 10 methods that dynamically choose the most abundant precursor ions from the survey scan (300–1800 m/z ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted with an Oasis PRiME HKB μElution Plate and analysed on a QExactive HF HPLC-MS (Thermo Scientific). Separation by HPLC prior to MS occurred on an Easy-nLC1200 system (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
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bioRxiv - Biochemistry 2023Quote: We acidified the digested samples with concentrated trifluoroacetic acid (TFA) and cleaned up the peptides with C18 SOLA Solid-Phase Extraction (SPE) columns (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were further separated onto an Easy-Spray RSLC C18 column (75 µm i.d., 50 cm length, ThermoFisher Scientific) using a 30 min linear gradient (15% to 35% solvent B (0.1% formic acid in acetonitrile) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 µm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
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bioRxiv - Microbiology 2023Quote: ... and peptide concentrations for each sample were estimated based on absorbance measurements at 280 nm (NanoDrop™, Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2022Quote: Peptide separation in buffer A (0.1% formic acid) was achieved using an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific) and a column (75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μg of peptides from each sample were reacted for 1 h with the appropriate TMT-NHS isobaric reagent (ThermoFisher) in 40% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with warmed PBS before the addition of peptide in DMEM (without phenol red) with 10% FBS (ThermoFisher) for 2-4 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by a top-15 data dependent LC-MS/MS strategy using a QExactive plus mass spectrometer (ThermoFisher). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Developmental Biology 2023Quote: ... Final peptide concentration was measured by absorbance at 280 nm on a Nanodrop ND-2000 spectrometer (Thermo Fisher, Waltham, MA).
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bioRxiv - Biochemistry 2023Quote: ... Each sample of peptides was loaded onto a C18 trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific) and then separated on a C18 analytical column (75 μm ID×50 cm ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested tryptic peptides were analyzed by LC-MS/MS on Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) using the same configuration and settings as previously reported85 ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to TiO2 spin tips (3 mg peptides per tip) for phosphopeptide enrichment according to the manufacturer’s protocol (High-SelectTM TiO2 Phosphopeptide Enrichment Kit, A32993, Thermo Scientific). Eluted phosphopeptides were transferred to Lo-bind Eppendorf tubes ...
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bioRxiv - Biochemistry 2023Quote: ... endoprotease Lys-C and Trypsin digested peptides were analyzed on an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Bremen). The raw data were analyzed with MaxQuant (version 2.0.3.0) ...
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bioRxiv - Microbiology 2023Quote: The enriched and unenriched samples were fractionated with the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer instructions for TMT-labeled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific) for 1 h at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50 cm × 75 μm ID; Thermo Fisher Scientific). Using a constant flow rate of 300 nL/min ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA encoding the mature protein (without the signal peptide) was amplified using gene-specific primers incorporating restriction enzyme sites and subcloned into the pPICZα A vector (Invitrogen) using EcoRI and XbaI enzymes and T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... 1 µg peptides were injected on an Ultimate 3500 RSLC coupled to a Q-Exactive HF-X (Thermo Fisher Scientific). Peptides were first trapped using a PepMap 100 C18 trap column and then separated with a PepMap C18 analytical column (75 µm x 50 cm ...
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bioRxiv - Physiology 2023Quote: ... with peptides introduced via an EasySpray Nano source coupled to a Q-Exactive Plus Quadrupole Orbitrap mass spectrometer (Thermo Scientific) and subjected to data dependent tandem mass spectrometry (MS/MS) ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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bioRxiv - Neuroscience 2023Quote: The peptides were analysed using liquid chromatography/mass spectrometry (MS) using with an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate3000 RSLCnano LC system (Dionex Co. ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic peptides were analyzed with a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated using an increasing acetonitrile gradient from 2% to 25% over 80 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Resulting tryptic peptides were separated by reverse-phase high-performance liquid chromatography (RP-HPLC; UltimateTM 3000 RSLCnano system; Thermo Scientific) using a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18 ...
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bioRxiv - Immunology 2023Quote: ... One microgram of peptide sample was loaded on a C18 column (15 cm, 3.0 μm Acclaim PepMap, Thermo Fisher Scientific), separated with a 0–40% gradient of buffer B (80% acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Fisher) and separated on a 50 cm Easy-Spray column (ES803 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was transferred to a new tube and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84688) following manufacturer’s recommendation.