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Citations for Thermo Fisher :
4201 - 4250 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Dried peptides were resuspended in MS loading buffer (3% ACN, 0.1 % FA) and measured with Q Exactive HF (Thermo Fisher Scientific) coupled to a reverse-phase nano liquid chromatography ACQUITY UPLC M-Class system (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... of pTyr peptides were carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher). Peptides were separated using a 140 min gradient with 70% acetonitrile in 0.2 M acetic acid at flow rate of 0.2 mL/min with approximate split flow at 20 nL/min ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Plant Biology 2021Quote: ... extracted peptides were separated on an EasyLC nano-HPLC coupled to a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) as described elsewhere (Kelstrup et al. ...
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bioRxiv - Microbiology 2021Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2021Quote: The LC-MS/MS analysis of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitated proteins were eluted from beads with 0.2 ml PDE storage buffer containing 1 mg/ml HA-peptide (Thermo Fisher Scientific) with mixing at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 3.5 kV distal voltage electrosprayed the eluting peptides directly into a Q Exactive HF mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... both preceded by a murine IgK leader peptide and followed by an 8xHis Tag were synthetized and cloned into pcDNA™3.1/Zeo(+) expression vector (Thermo Fisher). Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS (Thermo Scientific, San Jose, CA) for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization ...
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Plant Biology 2021Quote: ... Total peptide samples and the immuno-enriched fractions were then analyzed via LC-MS/MS using an EASY-nLC 1200 (Thermo Fisher) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... The lyophilized peptide was dissolved in nuclease-free water and its concentration determined on a Nanodrop 2000c (Thermo Scientific, Waltham, MA) using A280 with the molar extinction coefficient (3230 M-1cm-1 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Microbiology 2020Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn and separated on a 75 μm x 250 mm C18 column (PepMap ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were resuspended in 3% formic acid (FA)/5% acetonitrile (ACN) and loaded onto a nanoLC system (RSLCnano, Thermo Scientific). First ...
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bioRxiv - Microbiology 2020Quote: ... Eluting peptide fragments were ionized by electrospray ionization and analyzed on a LTQ-XL Orbitrap Discovery hybrid instrument (Thermo Fisher Scientific). The LC–MS/MS procedure was performed according to the data-dependent acquisition (DDA ...
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bioRxiv - Microbiology 2020Quote: ... The experimental setup was as follows: the peptide mixture was loaded onto a 75 μm × 2 cm precolumn (Acclaim PepMap 100, C18, 3 μm, Thermo Scientific) and eluted over 60 min using a 75 μm × 25 cm analytical column (Acclaim PepMap RSLC ...
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bioRxiv - Cell Biology 2021Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exac-tiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at flowrate of 2 00nl/min ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity enriched proteins were digested with trypsin and the resulting peptides desalted prior to LCMS analysis using an Easy-nLC 1000 system (Thermo Scientific) connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The peptides were trapped on a C18 PepMap 100 pre-column (300 μm i.d. x 5 mm, 100Å, Thermo Fisher Scientific) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Cell Biology 2020Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated peptides were then ionized using a nanospray ionization ion source and analyzed using an Orbitrap Fusion™ (Thermo Scientific) mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were eluted from the pre-column onto an Easy-spray (25 cm x 75 μm; Thermo Fisher Scientific, DE) column into the Orbitrap Exploris 480 mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at a flow rate of 200nl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Desalted peptides were solubilized in solvent A (0.1% aq. formic acid (FA)) and 1µg (determined by NanoDrop (Thermo Scientific Bremen, Germany)) was loaded and separated on an EASY-nLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated using a PepMap RSLC column (250 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) with gradient of 6-40% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated on a PepMap RSLC column (150 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) using a gradient of 5-30% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Molecular Biology 2022Quote: The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots containing 0.5 μg peptides were separated on a reverse phase C18 column and were analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 88 ...
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bioRxiv - Molecular Biology 2022Quote: ... The nTAP-MS peptides were applied on an Acclaim PepMap 100 C18 trap column (75 μm ID x 2 cm, 3 μm, Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... then the flow-throughs were fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive Plus, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Plant Biology 2022Quote: ... Tryptic peptides were resuspended in H2O containing 0.1% formic acid and separated in an EASY-nLC1000 chromatographic system (Thermo Fisher Scientific) for 95 minutes using a C18 nano-column under a constant flow of 400 nL/min and increasing concentrations of acetonitrile (7 to 30% over 70 min and then 70% in 25 min) ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were concentrated (online) by reverse phase chromatography using a 0.1 mm × 20 mm C18 RP precolumn (Thermo Fisher Scientific), and then separated using a 0.075mm x 250 mm C18 RP column (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Biochemistry 2022Quote: ... The latter was performed in the National Laboratory of Research and Services in Peptides and Proteins using an LCQ DUO ESI ion trap (Thermo Finnigan) or QExactive Orbitrap (Thermo Scientific) spectrometers.
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bioRxiv - Biochemistry 2022Quote: ... Relative ion abundance of peptides labelled with 12C-IPA and/or 13C-IPA in extracted ion chromatograms generated using XCalibur Qual Browser software (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...