Labshake search
Citations for Thermo Fisher :
4251 - 4300 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... An EASY nLC ultra-pressure liquid chromatography system was used to elute peptides onto a Fusion Tribrid mass spectrometer (Thermo Scientific). MS1 profiling was performed in a 375–1600 m/z range at a resolution of 70000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a μ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 μm i.d.×5 mm, 5 μm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
-
bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100Å (Thermo Fisher Scientific)) at a flow rate of 12 μl/min in 100% buffer A (0.1% formic acid in H20) ...
-
bioRxiv - Neuroscience 2021Quote: ... Phospho-LIMK1/2 was detected with rabbit polyclonal anti-phospho-LIMK1 Thr508/ LIMK2 Thr505 directed against a synthetic phospho-peptide (Thermo Fisher Scientific # PA5-37629 ...
-
bioRxiv - Biochemistry 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minute organic gradient ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting tryptic and chymotryptic peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). In brief ...
-
bioRxiv - Molecular Biology 2020Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, 90110) as described previously31 ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Molecular Biology 2020Quote: ... Peptides extracted from each band were analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Dionex, Thermo Scientific, Waltham, MA) coupled to a TripleTOFTM 6600 mass spectrometer (ABSciex) ...
-
bioRxiv - Cell Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min ...
-
bioRxiv - Microbiology 2020Quote: MS3-TMT: Analysis of TMT labelled peptide mixtures was carried out on the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) (control software Xcalibur™ ...
-
bioRxiv - Physiology 2021Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2021Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... A volume corresponding to 4 μg of the peptide mixture was separated by a Proxeon Easy-nLC System (Thermo Fisher Scientific) using an RP-C18-A2 column (3 μm) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and nuclear extracts were incubated for 1.5 hr at 4°C with 1 µg of H3K4ox (ART-K(OH)-QTARKSTGGKAP-biotin) or irrelevant (AHIVMVDAYKPTK-NH(CH2)2NH-biotin) peptides that had been previously recovered with streptavidin magnetic beads (Invitrogen; 65305) for 30 min at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
-
bioRxiv - Pathology 2021Quote: 1.5 μg of peptide was injected on an UltiMate 3000 nano LC system online coupled to a Q-Exactive HF-X instrument (Thermo Scientific). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
-
bioRxiv - Biochemistry 2021Quote: ... Peptides were ionized by nano-electrospray at 2 kV and analyzed on an Orbitrap Exploris 480TM (Thermo Fisher Scientific, Bremen, Germany) MS ...
-
bioRxiv - Biochemistry 2020Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 nl/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...
-
bioRxiv - Neuroscience 2022Quote: Peptides were constructed on a CEM Liberty 12-Channel Automated Microwave Peptide Synthesizer using Fmoc-PAL-PEG-PSTM (Rink resin; loading capacity 0.21 mmol/g) (ThermoFisher, Cat.#: GEN913383) and Fmoc-protected amino acids (GL Biochem (Shanghai)) ...
-
bioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (v/v) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Neuroscience 2022Quote: Chemicals and reagents specifically used for proteomic analysis include: Pierce™ high pH reversed-phase peptide fractionation kit (Cat. No.: 84868; ThermoFisher), High-Select™ Fe-NTA phosphopeptide enrichment kit (Cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 145 min and 99% MS solvent B at 150 min ...
-
bioRxiv - Genetics 2022Quote: Samples were suspended in 0.1% TFA and an equivalent to 1 µg of peptides was analyzed using Ultimate 3000 liquid chromatography system coupled to an Orbitrap QE HF (Thermo Fisher). An in-house packed analytical column (75 µm x 200 mm ...
-
bioRxiv - Microbiology 2022Quote: ... 25 μg of peptides from each sample were labeled with 0.25 mg of the TMTpro TMT-labeling reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: Peptide LC-MS analysis was performed with a Dionex 3000 nano-HPLC coupled to an Orbitrap XL mass spectrometer (Thermo Fisher). Peptide samples were pressure-loaded onto a home-made C18 reverse-phase column (75 µm diameter ...
-
bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
-
bioRxiv - Cancer Biology 2022Quote: ... was determined by measuring the phosphorylation of a FRET peptide substrate (Ser/Thr19) labeled with Coumarin and Fluorescein using Z’-LYTE™ assay (Invitrogen). The 10 µL assay mixtures contained 50 mM HEPES (pH 7.5) ...
-
bioRxiv - Plant Biology 2019Quote: ... pull-down and sample preparation was performed as described (Wendrich et al., 2017).c Peptides were applied to online nano LCMS/MS (Thermo Scientific) using a 60 minute acetonitrile gradient from 5-50% ...
-
bioRxiv - Molecular Biology 2019Quote: The purified digested peptides were separated with reverse phase liquid chromatography using a Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) and analyzed by tandem mass spectrometry using Orbitrap Fusion mass spectrometry (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... MpPHOT proteins were detected with rabbit antibody raised against the custom-made synthetic peptide (C+VDERAPPSKGSAKE) (Eurofins Genomics) and horseradish peroxidase-conjugated anti-rabbit IgG (Thermo Scientific). Both antibodies were used at dilution of 1:2000 ...
-
bioRxiv - Cell Biology 2019Quote: LC-MS/MS analyses of peptide mixture were done using RSLCnano system connected to Orbitrap Elite hybrid spectrometer (Thermo Fisher Scientific) with ABIRD (Active Background Ion Reduction Device ...
-
bioRxiv - Biochemistry 2020Quote: ... Injected peptide samples were on-line de-salted/concentrated using an Acclaim PepMap C18 trap column (75 μm × 2 cm, Thermo Scientific) for 2 min at 5uL/min using 2% MeCN + 0.2% FA ...
-
bioRxiv - Biophysics 2019Quote: ... Peptide amides were cleaved from the resin with 95% (v/v) aqueous trifluoroacetic acid solution (TFA, Fisher Scientific, Loughborough, United Kingdom) containing 5% (v/v ...
-
bioRxiv - Cancer Biology 2020Quote: Peptide fractions were analyzed by nanoLC-MS/MS on a Thermo Orbitrap Fusion hybrid mass spectrometer (Q-OT-qIT, Thermo Scientific) equipped with an EASY-NLC 1000 system (Thermo Scientific) ...
-
bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Biochemistry 2019Quote: ... The peptide samples were loaded on a nano viper trap column (C18, 5u, 100Å, 100um X 2 cm; PN: 164562, Thermo Scientific) and resolved on a 50 cm analytical column (2 um ...
-
bioRxiv - Biochemistry 2019Quote: ... The site of phosphorylation of 32P-labeled peptides was determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer of the peptide coupled to 80 Sequelon-AA membrane (Applied Biosystems).
-
bioRxiv - Plant Biology 2019Quote: ... Digested peptides were extracted from the gel by peptide extraction buffer (50% acetonitrile, 0.1% formic acid) and dried in a vacuum concentrator (Thermo Fisher Scientific). Peptides were resuspended in 0.1% formic acid and desalted using C18 ZipTips (Millipore ...
-
bioRxiv - Microbiology 2020Quote: Peptide samples were analyzed on an Orbitrap Fusion Tribrid mass spectrometer interfaced with Easy-nLC1200 liquid chromatography system (Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
-
bioRxiv - Cell Biology 2019Quote: LC-MS/MS analyses of peptide mixture were done using RSLCnano system connected to Orbitrap Elite hybrid spectrometer (Thermo Fisher Scientific) with ABIRD (Active Background Ion Reduction Device ...
-
bioRxiv - Molecular Biology 2021Quote: ... Peptides were measured on a QExactive HF Mass Spectrometer coupled to a Dionex UltiMate 3000 UHPLC system (both Thermo Fisher Scientific) equipped with an in house-packed C18 column (ReproSil-Pur 120 C18-AQ ...
-
bioRxiv - Genomics 2019Quote: ... Peptides in solution were dried by speed vacuum and reconstituted in 50 mM NH4OH and quantified by BCA (Thermo Fisher Scientific). Basic reverse phase fractionation was carried out on 8 μg of tryptic peptides using an XBridge BEH C18 column (130 Å ...
-
bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
-
bioRxiv - Immunology 2021Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37◦C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
-
bioRxiv - Systems Biology 2021Quote: ... lyophilized peptides were resuspended in 105 µl of equilibration buffer (1% TFA/ 80% ACN) and the peptide concentration was measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using equilibration buffer ...
-
bioRxiv - Immunology 2020Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37°C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... Dried peptide mix was resuspended in 2% TFA and fractionated using Pierce High pH Reversed-Phase Fractionmation kit (84868, ThermoFisher Scientific). Each fraction was vacuum-dried and resuspended in 25 μl 0.1% TFA ...