Labshake search
Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Cell viability was estimated by a quantitative colorimetric assay described for human granulocytes which were based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen) into coloured product formazan (Oez ...
-
bioRxiv - Developmental Biology 2022Quote: ... They were then immobilized on glass slides using 2% agarose and injected with 1,1’-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI, Molecular Probes) or 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with PBS and loaded with 3 µM Fura-2 AM (Thermo Fisher, Waltham, MA) diluted in a modified Krebs-Ringer buffer solution [KRBH ...
-
bioRxiv - Plant Biology 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm · 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm · 75 µm ...
-
bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
-
bioRxiv - Genomics 2024Quote: ... Fresh medium was added every 2-3 days and cells were passaged using 1X TrypLE Express (Life Technologies).
-
bioRxiv - Plant Biology 2023Quote: ... 3 µg RNA from each sample was used to mix with 2×RNA loading dye (Thermo Fisher Scientific), denatured at 65℃ for 10 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... or using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Fisher Scientific, AC158992500, Waltham, MA, USA) at 570 nm ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
-
bioRxiv - Pathology 2023Quote: Tail and ear fragments from mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
-
bioRxiv - Cancer Biology 2023Quote: Cell proliferation was assayed by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, M6494). MTT was freshly dissolved into PBS at a stock concentration of 12 mM and diluted into phenol red-free DMEM with 10% FBS for a final MTT concentration of 2 mM ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Mix 1 contains 3 siRNAs (CliniSciences, CRH7929) and Mix 2 contains two siRNAs (ThermoFisher Scientific, 1299001 and 4392420). As a negative control ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 μl of cDNA were mixed with 3 μl of PowerUp™ SYBR™ Green Master Mix (ThermoFisher) containing 1 μM of forward and reverse primer ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were passed every 2-3 days upon reaching ∼80% confluence using 0.05% trypsin-EDTA (Thermo Fisher Scientific) for dissociation.
-
bioRxiv - Molecular Biology 2024Quote: ... Other PCR products were run on 2 or 3% agarose gels containing Sybr Safe DNA stain (Invitrogen, #S33102) and imaged on a ChemiDoc imager.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific, M6394). After cell exposure to AG490 and MeHg at specified times and concentrations ...
-
bioRxiv - Cell Biology 2024Quote: ... and IPF fibroblasts underwent the 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay (ThermoFisher Scientific, M6494) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... and 3 were cleaved from IgGs using Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher) using protocols provided by the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
-
In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
-
bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
-
bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
-
bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
-
bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 minutes) at 4 °C and re-suspended in media composed of Minimal Essential Medium (MEM, Life Technologies) with 10%/vol fetal bovine serum (Omega) ...
-
bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
-
bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
-
bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
-
bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
-
bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
-
bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4 times before staining for 5 minutes with Pierce ECL Western Blotting Substrate (Thermo Fisher) as per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
-
bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cells were incubated with 5 µM Fluo-4 AM and 0.02% Pluronic F-127 (Thermo Fisher Scientific, P3000MP) in a buffer solution for 30 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 400µL of freeze buffer (50 mM Tris-HCl pH 8.0, 40% glycerol, 5 mM MgCl2, 0.5 mM DTT, 4 u/mL RNase inhibitor [SUPERaseIN, Invitrogen]) and stored at -80°C.
-
bioRxiv - Immunology 2024Quote: ... Cells were spun at 500G at 4°C for 5 minutes and resuspended in RNAlater ICE (Invitrogen AM7030).
-
bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
-
bioRxiv - Systems Biology 2020Quote: ... Disulfide bonds were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 hr ...
-
bioRxiv - Cell Biology 2020Quote: ... protected by an Acclaim PepMap C18 column (100 μm x 2 cm, 5 μm; Thermo Scientific) before injection into a Q-Exactive mass spectrometer (ThermoFisher) ...
-
bioRxiv - Microbiology 2020Quote: ... The precolumn was a 2 cm EASYcolumn (ID 100 µm, 5 µm particles) (Thermo Fisher Scientific) while the analytical column was a 10 cm EASY-column (ID 75 µm ...