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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Concentration of each sample was adjusted to 2 μg/μl and then mixed with 6 X SDS-containing Laemmli buffer (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... and these values were re-calibrated based on measurements of NIST-certified polystyrene beads (2 µm and 6 µm in diameter, Duke Standards, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... SVG-A Notch2-HaloTag cells were transfected in 6 well plate format with 2 µg of DNA per well using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells were seeded on a 6-well plate (2×105 cells per well) and were cultured in low glucose Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% sodium pyruvate ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were seeded at 5·105 cells/ml in a 6-well plate in a total volume of 2 ml of Sf-900-II SFM media (10902088, ThermoFisher Scientific). Bacmids (see above ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained with 1 mg/mL of 4’,6-diamidino-2-phenylindole (DAPI) and 0.1 nmol units/ml of Phalloidin ATTO647N and mounted with Fluoromount-G (Thermo Fisher Scientific). The slides were acquired using a confocal (Leica SP8 ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Cancer Biology 2023Quote: ... a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (Thermo Fisher). After gel transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... iPSCs were plated at high density (2 million cells/well in a 6 well plate) on hESC-qualified Geltrex (Gibco #A1413302), then cultured in neural induction media at high density with ROCK inhibitor Y27632 (StemCell Tech ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Microbiology 2024Quote: ... was used for the detection of genomic SARS-CoV-2 RNA through RT-qPCR using a thermocycler (QuantStudio 6 thermocycler; Applied Biosystems). Specific primers targeting the N-region viral gene ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight at 4 °C in primary antibodies (in PBS containing 2% bovine serum albumin (BSA)) : VHH-B07-Fc (at 6 µg/ml) and rat anti-αtubulin (1/200; ThermoFisher Scientific). After rinsing in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells grown on 6-well plates were transiently transfected with 2 μg plasmid DNA using 8 μl Lipofectamine 3000 (Invitrogen, #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: On day 6 post-infection mice were intravenously injected with 150 µL/mouse of 2% Evans blue (Fisher Scientific, Waltham, WA). After two hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Snap-frozen quadriceps muscles (n = 6 / group) were homogenized in 2% SDS lysis buffer and assessed for total protein concentration using Direct Detect (ThermoFisher Scientific). To verify total protein detection and adjust for loading ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was assessed 18 hours after UVA irradiation (λ = 365 nm, 6 and 12 J.cm-2) using Annexin-V/propidium iodide staining (ThermoFisher Scientific) and analyzed by flow cytometry according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 70 µm nylon colander (Falcon) with 3-4 ml of Ca2+- and Mg2+-free HBSS medium containing 10 % fetal bovine serum (FBS, Gibco), 1 % MEM-vit (Gibco ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... COCS were incubated for 3 days at 4°C with secondary antibodies Alexa594-conjugated goat anti-rabbit IgG (Life Technologies) or Alexa647-conjugated goat anti-rat IgG (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then centrifuged at 300 g for 3 min and fixed with 4 mM 3,3’– dithiobis(sulfosuccinimidyl propionate) (DTSSP, Life Technologies) in PBS at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: Group 3 MB cells (3×105) were seeded overnight and subsequently serum-starved for 4 hours prior to transient transfection using Lipofectamine 2000 (Invitrogen) for 6 hours in serum-free media with miR-212-3p mimic (25 nM and 50 nM ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM MEK inhibitor PD0325901 (Wako, Tokyo, Japan), 3 μM GSK3 inhibitor CHIR99021 (Wako, Tokyo, Japan) and Penicillin-Streptomycin (Invitrogen). Blastocyst outgrowths were disaggregated and passaged onto the new wells plated with feeder cells in the same medium condition ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... DTT concentration in the buffer was reduced by dialysis against TEDG buffer with DTT 0.1 mM at 4 ºC overnight using a 3 KDa pore size dialysis cassette (ThermoFisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... reinhardtii cells were grown at room temperature for 3-4 days in the light in 20 mL of TAP media (ThermoFisher). The algae were collected by centrifugation at 10,000 x g for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 1.3163 g/cm3) and ultracentrifuged for 3 hours at 180,000 × g (average) at 4°C (TH-641 rotor, Thermo Fisher, thinwall polypropylene tube with 13.2 ml capacity ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_10541045) for 3 days at 4°C followed by 2h in 1:800 anti-mouse FluoroNanogold (Life Technologies, A24920) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... were washed three times in sterile PBS and opsonized overnight at 4°C in 3 mg/mL mouse IgG (Invitrogen). To remove excess antibody ...
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bioRxiv - Microbiology 2020Quote: ... or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16, Thermo Fisher Scientific) in a modified SC medium ...
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bioRxiv - Plant Biology 2021Quote: ... TRV:NRC2/3/4 plasmid (Wu et al., 2016; Wu et al., 2017) by Phusion High-Fidelity DNA Polymerase (Thermo Fisher) using primers listed in SI Appendix ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed thrice in PBS and individually incubated with MCDB131 growth medium supplemented with 4 µM Fluo 3-AM (Invitrogen) for 25 min at 37°C ...