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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
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bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... lysates (∼5 x 10^6 cells per experiment) were pre-cleared with Pierce™ Protein A Agarose (Thermo Scientific™; Catalog No. PI20333) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed hearts were embedded in paraffin and sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific #630-0950). Immunohistochemistry was carried out using standard procedures ...
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bioRxiv - Biochemistry 2022Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 g for 30 minutes, A23-6 x 100 rotor, (Thermo Fisher Scientific, Waltham, MA). The supernatant was filtrated with a Filtropur BT50 0.2 µm bottle top filter (SARSTEDT ...
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bioRxiv - Bioengineering 2022Quote: ... Rat bone marrow mononuclear cells were cultured at 4-6 million in a 10cm dish (Falcon) using differentiation media containing: DMEM (ThermoFisher Scientific, Burlington, ON, Canada), 20% HI FBS ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Neuroscience 2021Quote: ... One million pre-differentiated cells were plated to each well of a Nunclon 6-well dish with 2 ml of the high purity differentiation media: DMEM/F12 (Thermo Fisher 21331020) containing high purity N2 (above) ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Neuroscience 2024Quote: ... wells were washed with PBS and a 2 nM mix of PS129 conjugated to Alexa Fluor 488 and Alexa Fluor 647 (ThermoFisher, A20181/6) applied for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were visually screened for the expression of single or sparsely labelled FoxP2.A:FingR(PSD95)+ neurons in the tectum using a 20x water-immersion objective and an LSM 980 confocal microscope with Airyscan 2 (Zeiss) and placed into individual wells of a 6-well plates (Thermo Fisher Scientific) to keep track of individual larvae and the corresponding labelled neurons ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2 micrograms of either CELSR constructs or empty vector DNA in a 6-well plate (Fisher Scientific FB012927) using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/min for 6 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical column ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/minutes for 6 minutes with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical columns ...
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bioRxiv - Developmental Biology 2024Quote: ... ECs isolated from each mouse were seeded in 2 wells of 6-well plate coated with attachment factor protein 1X (ThermoFisher Scientific, S006100). For a pure EC population ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells seeded on 6-well plates were transfected with 2 μg midi-prep quality plasmid DNA in Opti-MEM (Invitrogen, 31985-070) containing 2 μL Neofect™ DNA transfection reagent (TF20121201 ...
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bioRxiv - Molecular Biology 2024Quote: ... and SN3 fractions (in a volumetric ratio of 10:6:8) were mixed with 2× NuPAGE LDS Sample Buffer (Thermo Fisher Scientific). RNA was extracted from each fraction using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: The generation of reactive oxygen species (ROS) was quantified with the fluorogenic probe CM- H2DCFDA (6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate, acetyl ester) (Thermo Fisher Scientific). Cells were treated with inhibitors for 4h ...
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bioRxiv - Cell Biology 2020Quote: ... and optiMEM (1:6; Gibco) or were left untreated ...
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bioRxiv - Microbiology 2021Quote: ... 6% Sodium bicarbonate solution (Gibco), 8% Fetal bovine serum (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... TNF and IL-6 (Invitrogen) ELISA kits were used according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... or QuantStudio 6 (Applied Biosystems) thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... 6 μg/ml geneticin (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... TRAF-6 (Invitrogen-PA5-29622), and Cyclin D1 (Invitrogen-AHF0082) ...
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bioRxiv - Molecular Biology 2020Quote: ... IL-6 (Invitrogen, California, USA), TNF-α (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 mM L-glutamine (Gibco), 0.1 mM MEM non-essential amino acids (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 6 well plates (Biolite, Thermofisher) or 96 well plates (Biolite ...
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bioRxiv - Microbiology 2020Quote: ... 6 U Turbo DNase (Invitrogen) and 100 U DNase I (Roche ...
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bioRxiv - Microbiology 2020Quote: ... 6 mM L-glutamine (Gibco), 100 mg/ml penicillin and streptomycin (P/S ...
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bioRxiv - Microbiology 2020Quote: ... 6 mM L-glutamine (Gibco), 100 mg/ml penicillin and streptomycin (P/S ...
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bioRxiv - Cancer Biology 2022Quote: ... or QuantStudio 6 (Applied Biosystems) thermocycler ...
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bioRxiv - Cell Biology 2022Quote: ... on QuantStudio 6 (Life Technologies). Primers were pre-validated for high efficiency and internally tested not to vary upon OP9 differentiation ...
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bioRxiv - Microbiology 2022Quote: ... 6 days for blasticidin (Gibco), zeocin (Invivogen ...
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bioRxiv - Microbiology 2023Quote: ... 6 mM L-glutamine (Gibco), and 100 mg/ml penicillin and streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 × loading buffer (Thermo Scientific) was added and the reaction mixture was separated on a 1% agarose gel in 1x TAE (40 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µL Lipofectamine 2000 (Invitrogen) and 2 µg plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... 6% polyacrylamide gel (Invitrogen, EC68655BOX) and stained with SYBR-Gold (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 6% polyacrylamide gels (Invitrogen, EC68655BOX) to run in TBE (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... 6 mM L-glutamine (Gibco), 25 µg/mL G418 Sulfate (Gibco)] ...
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bioRxiv - Microbiology 2024Quote: ... 6 mM L-glutamine (Gibco), 100 μg/ml penicillin and streptomycin (P/S ...