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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and 143B cybrid cells were passaged every 3-4 days at 80% confluence and trypsinised using 0.25% trypsin-EDTA (Gibco #25200056). Similarly ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Immunology 2021Quote: ... A force of -4 was used to blot excess liquid for 3 s using a Vitrobot Mark IV (Thermo Fisher) followed by plunge-freezing with liquid ethane ...
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bioRxiv - Immunology 2020Quote: ... The excess liquid was blotted for 3 seconds with a force of −4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... the same amounts of SOSIP trimer and SOSIP-I53-50NP were loaded on a 4-12% Bis-Tris NuPAGE gel or 3-12% Bis-Tris NuPAGE gel (both from Invitrogen), respectively.
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... For differential glucose concentration treatments between day 2 and 3 and between day 3 and 4, N2B27 was made using DMEM/F12 without glucose (L0091-500, Biowest) and Neurobasal without glucose (A2477501, ThermoFisher). For 1X and 3X glucose concentrations ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Neuroscience 2022Quote: ... slices for optical recordings were incubated for 30 minutes in oxygenated Krebs solution added to a 3% Di-4-ANEPPS (Invitrogen) stock solution and mixed with an equal volume of fetal bovine serum (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA samples of 4 ng were subjected to DNaseI treatment and 3’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) and T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Molecular Biology 2022Quote: ... The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells, 288 reactions in 3×96-well plates) following manufacture’s protocol (Invitrogen C862003). After transformation ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The cells were passaged every 3-4 days after they achieved confluency of 80-90% using Trypsin 0.25% EDTA (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... LC-MS/MS based proteomics datasets (4 groups × 3 replicates) were then generated in a lab (code: NVG) using Q Exactive HF-X mass spectrometer (ThermoFisher). To eliminate confounding factors such as experimental sample-processing order with sample group ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Cell Biology 2024Quote: ... islets from 3-4-week-old control and β-LonP1KO mice were handpicked and then dispersed with trypsin (Thermo Scientific). Islet cells were counted and incubated with adenovirus expressing an empty vector control (Ad-RIP2 EV ...
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bioRxiv - Pathology 2024Quote: ... retinas were post-fixed in 4% PFA for 3 minutes at room temperature before mounting in Prolong Gold antifade medium (Invitrogen). Images were acquired using a Zeiss LSM710 and Zeiss LSM880 confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... 30 to 50 μg of protein extracts were resolved by SDS-polyacrylamide gel electrophoresis using 4–12% NuPAGE Bis-Tris gradient gel or 3–8% Tris-acetate gel and run (Invitrogen) and MOPS or Tris-acetate running buffer (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... red blood cells were removed by adding 1 ml ACK lysis buffer (150 mM NH4Cl and 10 mM KHCO3) and incubated on ice for 3 minutes before washing with HBSS containing 4% fetal bovine serum (HI FBS, Gibco). During the optimization of the protocol outlined above ...
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bioRxiv - Cell Biology 2024Quote: ... 1 or 3 mg of protein was incubated for 1 hour at 4°C with 10 μl pre-washed dynabeads (Invitrogen) to clear the lysate from unspecific interactions ...
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bioRxiv - Genetics 2024Quote: ... Proteins were loaded and run on a 4-12% Bis-Tris pre-cast gel or a 3-8% Tris-Acetate pre-cast gel (Invitrogen) and were electrophoretically transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were usually passaged every 3-4 days in a 1:10 ratio using 0.05% (v/v) trypsin (Gibco, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured neurons were incubated with 3 μg/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) in external recording solution for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... was aspirated and the fixed cells treated with 2-3 drops of ProLong Gold Antifade Mountant (Invitrogen #P36930) before imaging ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed for 2-3 min in water and transferred to a chambered cover glass (Thermo Scientific), and imaged either as described above or using a Leica DM5500 wide field microscope (GFP filtercube ex ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen] ...
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bioRxiv - Microbiology 2020Quote: ... Myoblat and myotubes viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT; Life Technologies) metabolization.
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bioRxiv - Cancer Biology 2020Quote: ... then assayed in a standard MTT assay: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher, Waltham, MA) [39] ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... equipped with an Acclaim Pepmap C18 trap column (2 cm * 75 µm, particle size: 3 µm; Thermo Fisher) and a C18 analytical column (50 cm * 75 µm ...
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bioRxiv - Genetics 2021Quote: ... Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific) prior to protein transfer to Amersham Protran nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with NeuroCult™ SM1 neuronal supplement (STEMCELL), L-glutamine (2 mM, PAA) and 3% horse serum (Invitrogen) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: Tail and ear fragments from C57/B6 or mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...
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bioRxiv - Cell Biology 2020Quote: ... and induced to differentiate in myotubes for 2-3 days in differentiation media (DM: IMDM (Gibco, 21980-032) + 2% of horse serum (Gibco ...