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Citations for Thermo Fisher :
3901 - 3950 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at 100% density in 6-well plates in NMM medium (50% DMEM/F-12 + GlutaMAX (Thermo Fisher, 31331093), 50% NBM ...
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bioRxiv - Molecular Biology 2024Quote: ... patient-derived immortalized myoblasts and non-DUX4 expressing cells (54-6; 13 D4Z4 repeat units) were grown in Ham’s F-10 Nutrient Mix (Gibco, Waltham, MA) with 20% FBS (Corning ...
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bioRxiv - Evolutionary Biology 2024Quote: ... washing was performed again but cells were resuspended in 12mL of HNM+80μg/mL and plated across a 6-well plate (Thermo Scientific; 140685). Cells were then incubated for 72 hours at 22oC and 60% humidity ...
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bioRxiv - Genomics 2024Quote: 20 μg of retroviral plasmid DNA (sgGFP-Cr2 or sgZbtb20-Cr3-cs2) with 6 μg pCL-Eco plasmid DNA were transfected using 60 μl Lipofectamine 2000 (Invitrogen, #1668019) in 3 ml OptiMEM (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Scaffolds or 6 mm punches of dissected fresh mouse cortical brain tissue were placed on a Fisherbrand double frosted microscope slide (Fisher Scientific) in the center of an adhesive ...
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bioRxiv - Biochemistry 2024Quote: ... The wells were then washed 6 times with wash buffer and subsequently incubated with 100 µL per well of 1:10,000 streptavidin-HRP (Thermo Scientific 21130) in dilution buffer for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 80-90 % of cell cultures were split into a ratio of 1/3 to 1/6 using Tryple Express Enzyme (Gibco, 12604021) and Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Immunology 2024Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of Gapdh mRNA ...
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bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2024Quote: ... Purified CD14+ monocytes were seeded at a density of 1 million cells/well in 6-well plates in RPMI 1640 (Life technologies) with 10% serum and 20μg/ml recombinant human MCSF (Peprotech ...
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bioRxiv - Microbiology 2024Quote: RNA was collected from mmIFNα1 treated or untreated cells grown in 6-well plates 17 hours after treatment using TRIzol (Thermo Fisher Scientific). cDNA was then generated using SuperScript III reverse transcriptase and oligo-dT primers (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: TR146 cells grown in antibiotics-free media at ∼70% confluence in 6-well plate were transformed with lipofectamine RNAiMAX reagent (Invitrogen, #13778) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2.5 µg (6-well plates) of plasmid DNA diluted in OptiMEM medium using the Lipofectamine 3000 reagent (Invitrogen, ThermoFisher Scientific, France) for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... ten-fold serial dilutions from 10-1 to 10-6 of media from infections were prepared in 1 x PBS (Gibco, #14190250). The media on Vero cells seeded the previous day was aspirated ...
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bioRxiv - Bioengineering 2024Quote: ... the scalp was shaved before each session and the transducer affixed to the animal’s head via implanted titanium pins (Gray Matter Research, USA) and coupled using a 6% polyvinyl alcohol (Fisher Scientific, USA) cryogel ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Microbiology 2021Quote: ... TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: 143B cells were plated into a 6-well plate and transfected with 100 nM of miR-34c mimic or negative miRIDIAN mimic using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the ImageJ software (26) ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955], in H2O [Fisher Scientific, W6] ...
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bioRxiv - Microbiology 2020Quote: ... trachomatis-infected cells by scraping one or two wells of a 6-well tissue culture plate in a total volume of 500 μL Trizol Reagent (Thermo Fisher Scientific). Samples were transferred to RNase-free o-ring capped tubes containing ~100 μL volume of zirconia beads and thoroughly vortexed for 10 min to rupture bacterial cells ...
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bioRxiv - Microbiology 2019Quote: Spotted protein arrays (5 x 6) were printed onto each well of Greiner high-binding 96-well plates (Cat# 655097, Thermo Fisher Scientific) using a sciFLeXARRAYER S12 (Scienion AG ...
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bioRxiv - Molecular Biology 2021Quote: For DNA plasmids: 5×106 HEK293T cells were grown in 6-well plates and Lipofectamine™ 3000 Transfection Reagent (L3000001, ThermoFisher UK) was used to transfect the cells following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for anti-eEF3 and anti-G-6-PDH secondary antibodies and developed with Pierce ECL plus western blotting substrate kit (Thermo Scientific 32132).
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Immunology 2020Quote: ... The peptide was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 47 ...
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Exon 2 of A3A and exons 5 and 6 of A3B with either 20 or 80 bp of flanking intronic sequences were synthesized (Thermo Fisher Scientific) and cloned in sense orientation using XhoI and NotI restriction sites of Exontrap vector pET01 (MoBiTec ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were detected by electrophoresis on 6% non-denaturing polyacrylamide/TBE gels with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher Scientific). Splicing efficiency was measured by densitometry analysis of the PCR amplicons in image J (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... DAB-developed samples were stored in 70% glycerol and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, #D3571, 1:1000) nuclear marker ...
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bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Slides were then washed as above and incubated in 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DAPI (4-6-diamidino-2-phenylindole) and DyLight 488-or DyLight 555-labeled secondary antibodies (1:400, Thermo Fisher Scientific, Waltham, MA) were added for 1.5h at room temperature ...
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bioRxiv - Immunology 2022Quote: BMDMs were seeded into 6-well plates (one million cells per well) in DMEM containing 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM unlabeled glutamine ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR products were fractioned by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (Thermo scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ 1.47v (NIH ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μl of FuGENE® 6 and 1.3 μg of plasmid were added to 100 μl of OPTI-MEM Medium (Thermo Fisher Scientific). The mixture was incubated at room temperature for 30 minutes prior to the addition to the coverslips.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Biophysics 2019Quote: ... Cells were harvested by centrifugation at 7,800 g for 10 minutes in an F9 6×1000 LEX rotor (Thermo Scientific, Waltham MA), using a Lynx 6000 centrifuge (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... Primer pairs producing a single product band of expected size during PCR amplification were shortlisted for fluorescent dye labelling (forward primers) with one of four fluorescent dyes (6-FAM, VIC, NED, or PET, Invitrogen, South Korea) to perform fragment analysis using Applied Biosystems 3130 Genetic Analyser ...
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bioRxiv - Cell Biology 2019Quote: ... SYBR Green qPCR experiments were performed following the manufacturer’s protocol using a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems, Waltham, MA). cDNA (30 ng ...
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bioRxiv - Developmental Biology 2019Quote: Samples were divided into two groups— peripheral blood- and cord blood-derived cells— such that each set could be separately labeled with TMT 6-plex isobaric mass tagging reagents (Thermo Fisher Scientific) as previously described (41) ...
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bioRxiv - Microbiology 2019Quote: ... medium supplemented with 10 µg/ml gentamicin was added to HIEC-6 cells grown to confluency on 12 mm coverslips (Thermo Fisher Scientific) in 24-well plates (Corning Inc. ...