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Citations for Thermo Fisher :
3801 - 3850 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Primary human macrophages were generated by differentiating blood-derived CD14+ monocytes for 6 days in Roswell Park Memorial Institute (RPMI)-1640 medium (ThermoFisher Scientific) containing 10% HyClone FBS (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR assays were performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). The TaqMan Universal PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Growth medium on HEK293T cells at 90% confluency in 6 well plates was replaced with Opti-MEM reduced serum medium (Thermo Fisher) and cells in each well were transfected with 250ng each of 8 pDUAL reverse genetics plasmids ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: The PCR products (6 µl per well) were confirmed through 96-well E-gel with SYBR (Thermo Fisher, Catalog no G720801) using 25 µl of loading buffer (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse-transcription-PCR (qRT-PCR) was performed on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems A43182) in 384-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... SVG-A Notch2-HaloTag cells were transfected in 6 well plate format with 2 µg of DNA per well using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Immunology 2023Quote: ... Monocyte-derived macrophages (MDM) were generated by seeding three million CD14+ cells into one 6-well plate (NuncTM Thermo Fisher Scientific) in IMDM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed using the PTFE filters and resuspended in 1x PBS or further biotinylated with 6 mg/ml EZ-link™ Sulfo-NHS-SS-biotin (#21331; ThermoFisher). Streptavidin conjugated to either AlexaFluor-488 or -568 was then added for 1h RT incubation ...
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bioRxiv - Bioengineering 2023Quote: ... Sigma-Aldrich 7722-84-1) and then slowly injecting 0.03 % w/v sodium dithionite (ACROS Organics 7775-14-6, in PBS) using a syringe pump (10 µL min−1 ...
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bioRxiv - Cancer Biology 2023Quote: ... a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (Thermo Fisher). After gel transfer ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... reprogramming was continued in E8 medium and from Day 6 to Day 18 in E7 medium (E6 medium, cat. A1516401, Thermo Fisher Scientific + 100ng ml-1 bFGF ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of plasmid and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™), incubated for 30 minutes at room temperature and added dropwise to plates containing cells ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells were seeded on a 6-well plate (2×105 cells per well) and were cultured in low glucose Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% sodium pyruvate ...
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bioRxiv - Bioengineering 2023Quote: ... the hydrogel is first activated with sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Pierce, Thermo Fisher Scientific, Waltham, MA). Here the remaining washing buffer around the gel edge is removed to ensure the coating solution can be held on top of the gel ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.6 µm particle size column mounted in a Vanquish LC coupled to an Orbitrap ID-X Tribrid mass spectrometer (ThermoFisher Scientific). The mobile phases and chromatographic gradients used are described in Supplementary Table S6 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 ng of cDNA was used to perform the qRT-PCR reaction using SYBR green PCR master mix on an ABI Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems). The housekeeping genes GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 KELLY or 5 x 105 TET21N cells were transfected on a 6-well plate using 7.5 µl Lipofectamine RNAiMAX (Thermo Fisher Scientific) and 50 pmol siRNA according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are cultured for 3 days in a 6-well plate before they are gently detached with 10-min incubation in an enzyme-free cell dissociation buffer (Gibco, 13151014). These detached cells are then replated on ECM-coated substrates and imaged after 6 hrs.
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bioRxiv - Immunology 2023Quote: Spleen cells from CD45.1+ CD45.2- male and CD45.1- CD45.2+ female C57BL/6 wild type mice were harvested as described above and stained with Cell Trace Violet cell proliferation kit (Molecular Probes) in PBS at different concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed in MicroAmp optical 384-well plates using the QuantStudio 6 Flex RT-PCR system (Thermo Fisher). The default thermocycling program was used (95ºC for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: Cell sizes of mouse and rat embryonic molars were determined by staining 6 µm thick histological sections with DiI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... washed 1x with PBS and plated at 200,000 cells per well in 6-well plates coated with matrigel in iMGL media [DMEM/F12 (Thermo Fisher Scientific), ITS-G (2% v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were seeded at 5·105 cells/ml in a 6-well plate in a total volume of 2 ml of Sf-900-II SFM media (10902088, ThermoFisher Scientific). Bacmids (see above ...
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bioRxiv - Biochemistry 2023Quote: ... cells from 100 mL of culture were resuspended and chemically lysed for 30 min in 6 mL of B-PER II (Thermo Fisher) diluted to 1x with TEMB buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... jaw specimen undergone silver deposition and gold enhancement was casted in 6 % low melting point agarose (TopVision, ref R0801, Thermo Scientific) in 1xPBS and sectioned to 100 µm sections with vibratome (Microm) ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was completed using the SYBR green method carried out on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using LabTaq Green Hi Rox (Labtech) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained with 1 mg/mL of 4’,6-diamidino-2-phenylindole (DAPI) and 0.1 nmol units/ml of Phalloidin ATTO647N and mounted with Fluoromount-G (Thermo Fisher Scientific). The slides were acquired using a confocal (Leica SP8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and size exclusion chromatography (SEC) using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was propagated in C6/36 Aedes albopictus cell line at a multiplicity of infection (MOI) of 0.01 and cultured for 6 days in Leibovitz’s L-15 medium (Thermo Fisher Scientific, 11415064) supplemented with 0.3% tryptose phosphate broth (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT PCR was performed as described previously (Li et al, 2018) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). SsoFast EvaGreen Supermixe (Takara ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 293T human cells were seeded onto coverslips in a 6-well plate followed by transfection with the GFP plasmids noted above using Lipofectamine 3000 reagent (Thermo Fisher). For mitochondrial localization ...