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Citations for Thermo Fisher :
3751 - 3800 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 10 min at 95 °C followed by 40 cycles of 10 sec at 95 °C and 40 sec at 60 °C using the Quant 6 real-time PCR instrument (Applied Biosystems). Samples were considered positive for Giardia spp ...
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Microbiology 2024Quote: ... initial DNA libraries (including 6-12 degenerate NNK codons) were transcribed to mRNA using T7 RNA polymerase (37 °C, 16 hr) (Thermo Scientific) and ligated to a puromycin linker primer ([5’Phos]CTCCCGCCCCCCGTCC[SP18][SP18][SP18][SP18][SP18]CC[Puromycin] ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... and the RNA product purified from a 6% (vol vol-1) PAA-7M urea gel using a LowRange RNA ladder (ThermoFisher Scientific) for precise sizing ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in culture supernatants was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Cell Biology 2023Quote: ... UNC45A-GFP overexpressing RFL-6 cells (16 hrs post-infection) were treated with Tubulin Tracker Deep Red (Thermo Fisher Scientific T34077) and imaged as we have previously described [13] ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the individual larval zebrafish and 6-month-old adult brain samples using the mirVana™ miRNA Isolation Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were dissociated using Accutase and seeded at a density of 5.5 x106 cells per well in ultra-low attachment 6-well plates (Thermo Fisher Scientific) in E8 medium supplemented with 10 μM ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific, A3890401) and 200 μg/ml human JAGGED1-Fc ...
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bioRxiv - Genomics 2023Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan assays were used to quantitate ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Immunology 2023Quote: ... a total of 6 – 9 consecutive 10-µm coronal sections were taken from of 4 – 6 equally distanced regions along the length of the spinal cord using a Shandon Finesse 325 microtome (Thermo Scientific). Sections were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The grids were blotted with filter paper for 6 s and immediately plunged into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 4 °C and 95% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... 500,000 cells were plated in 6-well dishes and 1 μg of DNA was transfected when cells reached 75-85% confluency with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... PBS was replaced with a 1:6 (weight: volume) solution of 0.5 M acetic acid (984303; Thermo Fisher, Waltham, MA, USA) with 1 mg/ml pepsin (20343 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg plasmid DNA per well was transfected into cells for 16 h at a confluency of 60% in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse IL-1β biotin conjugate (13-7112-81), and anti-mouse IL-6 (MP5-20F3, 14-7061-85) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by medium-speed sedimentation at 10,000xg for 15 min using a fixed angle FIBERlite F14-6 x 500 y rotor (Thermo Fisher Scientific). The supernatant fraction was then centrifuged at 32,000 RPM for 1.25 h in a SW32 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were performed using HOT FIREPOL EvaGreen Supermix (Solis BioDyne) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Every sample was analyzed in triplicate ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Neuroscience 2023Quote: ... HBMECs were seeded in 6-well plates and at 75% confluency were stained for 20 min at RT using a crystal violet (Fisher Scientific) fixing (37% formaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... and stained the same day of the experiment with the fluorescent dye 5(6)-TAMRA/SE™ (Thermo Fisher Scientific, C1171) at a final concentration of 1 μM (as in Wehrl et al ...
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bioRxiv - Physiology 2023Quote: ... 36 hours upon transfection DMEM-F12 medium in 6-well plates was replaced with fresh DMEM/F-12 medium without phenol red (Gibco #21041025). 48 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: N2A cells were seeded at 300,000 cells per well in a 6 well plate 24 hours before transfection using Lipofectamine 3000 (Thermo Fisher Scientific). Transfections were performed in accordance with the Lipofectamine 3000 protocol ...
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bioRxiv - Plant Biology 2023Quote: Heat-stress (HS)/LaCl3 experiments: 6-day old cells (20 mL in 100 mL flask) were treated with LaCl3 (Thermo Scientific) (750 µM ...
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bioRxiv - Genetics 2023Quote: Whole cell extracts of 2D cultures from one well of a 6-well plate were prepared using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Cell Biology 2023Quote: ... and ProLong™ Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (P36941) or without (P36930) was purchased from Invitrogen.
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bioRxiv - Immunology 2023Quote: ... cDNA from samples was mixed with QuantiTect probe PCR master mix and gene-specific primers to run on a QuantStudio 6 Flex real-time cycler (Applied Biosystems). To generate a control for the amplification reaction ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... on day -2 fibroblasts were seeded in increasing concentrations on two 6-well plates (10-35 x 104 cells/well) with reprogramming fibroblast medium (PFM; DMEM, 10% ESC-qualified FBS (Thermo Fisher), 1% NEAA (Thermo Fisher) ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged every 5-6 days as small clumps by dissociation with a buffer containing 1 mg per ml Collagenase IV (ThermoFisher Scientific), 0.025% Trypsin (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mNat2 mRNA expression levels were determined by quantitative polymerase chain reaction (qPCR) using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The cDNA was amplified using primers for mNat2 variant 2 and mGAPDH (see Supplementary Table S5 for details ...
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bioRxiv - Cancer Biology 2023Quote: ... The expression levels of selected genes were determined using qRT-PCR (QuantStudio 6, ThemoFisher Scientific) using the SYBR Green Real-time PCR master mix (Thermo Scientific). The sequence of the primers used for the gene expression analysis was given in an appendix.
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bioRxiv - Cancer Biology 2023Quote: ... The B6-LSL-Cas9-eGFP keratinocytes were transiently transfected with 1μg of a CRE-expressing plasmid in 6-wells using Lipofectamine 2000 (Invitrogen; 11668-027) to activate Cas9-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were transfected with PNKP-FLAG (WT and mutants) expressing vector (pcDNA3) in 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmid DNA containing the construct of interest was transfected into 2mL of Sf9 cells at a density of 1×106 cells/mL on a 6-well plate using ExpiFectamine Sf Transfection Reagent (Life Technologies) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Neuroscience 2023Quote: ... and then starved in 50 µl EMEM-Serum free medium (antibiotic free) in presence or absence of a protein kinase inhibitor (PKI) (6-22amide, 50 nM, GIBCO BRL) for 3 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed in technical quadruplets on a QuantStudio 6 Flex Real-Time 384-well PCR System (Applied Biosystems, 4485694) using PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...