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Citations for Thermo Fisher :
4101 - 4150 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... trachomatis-infected cells by scraping one or two wells of a 6-well tissue culture plate in a total volume of 500 μL Trizol Reagent (Thermo Fisher Scientific). Samples were transferred to RNase-free o-ring capped tubes containing ∼100 μL volume of zirconia beads and thoroughly vortexed for 10 min to rupture bacterial cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and filamentous actin with Alexa Fluor 488-conjugated phalloidin (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The syn-gRNA oligos and syn-gRNA-NTC were transfected using Lipofectamine RNAiMAX transfection reagent (5 µl per 6-well; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific, R0533) diluted in serum-free media ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... MDA-MB-231 cells were seeded at a density of 500,000 cells/well in a 6-well plate and transfected with siRNA targeting EED using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) for 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were then washed with 1x PBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306), followed by mounting as described above.
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bioRxiv - Immunology 2022Quote: ... Bacteria were harvested after 16–18 h at 3000 x g for 15 min at 4°C (Sorvall LYNX 6000 centrifuge, F9-6×1000 LEX rotor, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with DPBS before being stained with 50 μL/well of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) (Invitrogen, cat. #D1306) diluted to 5 μM in DPBS for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Relative transcript abundances of the selected genes were calculated with the ddCt method (52 ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded at 3 μl/min for 6 min onto a 2 cm × 75 μm C18 trap column (Acclaim Pepmap 100, 3 μm, 300 Å, Thermo Scientific) in loading buffer (0.5% v/v formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... A 60-70% confluent 6-well plate of undifferentiated hPSC colonies was lifted from the feeder layer using collagenase (Thermo Fisher, 17104019). Colonies were transferred to a 10 cm dish containing hES medium without fibroblast growth factor on a shaker (+/− 50 RPM) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK293T cells were seeded at the density of 106 cells/well in a 6-well plate and were grown overnight in complete DMEM/F12 1:1 Glutamax ™ (Thermofisher, UK). On the next day ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Neuroscience 2023Quote: ... The protein pellets were resuspended in 6 M guanidine solution prepared in 100 mM triethylammonium bicarbonate (TEAB) buffer (Thermo Scientific, Cat# 90114). The protein solutions were reduced with 5 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Acute transverse hippocampal slices (250 µm) from wild-type C57BL/6 mice were prepared on a vibratome (Microm HM600V, Thermo Scientific, France) in ice-cold dissecting solution containing (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... C57BL/6 P4-5 mice pups were rapidly decapitated and brains were processed in Hibernate-A medium (Thermo Fisher Scientific, A12475-01). Resulting tissue was enzymatically disassociated in buffer containing HEPES-HBSS with DNase (Worthington Biochemical LS002007 ...
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bioRxiv - Neuroscience 2022Quote: Quantitative RT-PCR was performed using Taqman Gene Expression Assays and Taqman Fast Advanced Mastermix on a QuantStudio 6 Flex system (Thermo Fisher Scientific). Relative gene expression was determined via the ΔΔ-ct method normalised to GAPDH (human tissue) ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: 100,000 LX2 or 20,000 RAW cells were seeded in 6- and 12-well tissue culture plates and allowed to grow for 24h in DMEM (GIBCO, Life Technologies) containing 10% FBS and antibiotics ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: DRG were harvested from thoracic spinal levels of adult Pvalbcre;Rosa26Ai14 and Pirtcre;Scn1a-floxed (6-16 weeks) mice of both sexes and transferred to Ca2+-free and Mg2+-free HBSS solution (Invitrogen, 14170-112). Upon isolation ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Microbiology 2022Quote: ... After 2 washes with 1xPBS, the cell nuclei were stained with NucBlue Fixed Cell Stain ReadyProbes Reagent (4’,6-diamidino-2-phenylindole, DAPI) (Thermo Fisher Scientific) for 30 min in the dark at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... NC cells were aggregated into 3D spheroids (5 million cells/well) in Ultra Low Attachment 6-well culture plates (Fisher Scientific, 3471) and cultured in Neurobasal (NB ...
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bioRxiv - Cell Biology 2022Quote: ... myotubes were transfected with 1µg of the MHC I (H2-kb) overexpression plasmid using Lipofectamine™ 2000 reagent for a period of 6 hours (Invitrogen, USA), both without and with the addition of the ER stress blocking agent ...
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bioRxiv - Cell Biology 2023Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Single-axis tilt series were collected covering an angular range from -65° to +65° or -60° to +60° at 6- to 8-μm underfocus using the Tomography software package (Thermo Fisher Scientific). Subtomogram averaging was done the eman 2.99 software package ...
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was then used for real-time qPCR with the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) using the Quant Studio™ 6 Flex Real-Time PCR system (Thermo Fisher). The mRNA levels of GAPDH or actin were used as an internal control to normalize the mRNA levels of genes of interest ...
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bioRxiv - Genomics 2024Quote: Probes were hybridized to the sample at a concentration of 6-10 µM in a 30% v/v formamide (Fisher Scientific, AM9342), 10% w/v dextran sulfate (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... wells were washed with PBS and a 2 nM mix of PS129 conjugated to Alexa Fluor 488 and Alexa Fluor 647 (ThermoFisher, A20181/6) applied for 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Sciatic nerves of 6 mouse pups between postnatal days 2 and 4 (P2 and P4) were collected in L15 medium (Thermofisher, Massachusetts, USA) and incubated for 30 min in 2 mg/mL collagenase II and then 10 min in 0.25 % trypsin containing EDTA at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: Cells seeded at a density of 1.75×105 cells per well in a 6-well plate were transfected with 40 nM of pooled anti-IL6ST (cat# s7317, s7318, s7319; Thermo Fisher Scientific) or non-targeting Silencer Select siRNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... uteri were cut open and dissociated in 5mL Hanks’ Balanced Salt Solution (HBSS) containing 6 mg/mL dispase (Thermo Fisher, Waltham, MA) and 1% (w/v ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: All RT-qPCR experiments were performed on an Applied Biosystems QuantStudio 6 system in 96- well plate format using PowerUp SYBR Green Master Mix (ThermoFisher, cat#A25741). Prior to use in experiments ...
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bioRxiv - Biophysics 2024Quote: Quantitative determination of intracellular pH (pHi) was performed using the cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid AM (Thermo Fisher) in live imaged N2a cells at 48 hours of differentiation ...
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bioRxiv - Developmental Biology 2024Quote: ... Sucrose gradient fractions were monitored at 254 nm absorbance on an UA-6 UV/VIS detector (Teledyne Isco Inc., Lincoln, NE) and collected in individual tubes containing TRIzol LS (Ambion, Austin, TX).
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bioRxiv - Immunology 2024Quote: Individual cytokines in supernatants from cell culture experiments were measured using the species-appropriate ELISA Kits mouse IL-6 (Invitrogen™ 88706488), mouse IL-1beta (Invitrogen™ 88701388) ...
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bioRxiv - Genomics 2024Quote: TSCs wild-type and CRISPR-Cas9 edited cells were grown separately in wells of a 6-well plate and harvested at 80% confluency (∼2e6 cells) using 500 μl of TRIzol reagent (Life Technologies Corp). The lysate was incubated for 5 min on ice ...