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Citations for Thermo Fisher :
3851 - 3900 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (Thermo Fisher). After gel transfer ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Cell Biology 2023Quote: ... and ProLong™ Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (P36941) or without (P36930) was purchased from Invitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT PCR was performed as described previously (Li et al, 2018) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). SsoFast EvaGreen Supermixe (Takara ...
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bioRxiv - Neuroscience 2023Quote: Aggregates from four wells of a 6-well plate were transferred to one 175 cm2 tissue culture flask (Thermo Scientific, #159926) previously treated with an anti-adherence solution (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher Scientific) supplemented with 20% fetal bovine serum and 1% pen-strep ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... Grids were blotted for 6 s with a blotting force of 6 at 4 °C and 100% humidity and plunged into liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher). The data were collected with a Glacios Cryo-TEM (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the individual larval zebrafish and 6-month-old adult brain samples using the mirVana™ miRNA Isolation Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan assays were used to quantitate ...
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bioRxiv - Microbiology 2023Quote: All transfections were performed on 293T cells (seeded at a density of 0.5 × 106 cells/well of a 6-well plate one day prior) using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendation unless stated otherwise ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Cell Biology 2023Quote: ... 10 μg total RNA was denatured in formamide loading dye and resolved on the 6% TBE-Urea gel (Novex, ThermoFisher Scientific) in 1 X TBE buffer ...
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bioRxiv - Cell Biology 2023Quote: ... on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific, A3890401) and 200 μg/ml human JAGGED1-Fc ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were dissociated using Accutase and seeded at a density of 5.5 x106 cells per well in ultra-low attachment 6-well plates (Thermo Fisher Scientific) in E8 medium supplemented with 10 μM ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... UNC45A-GFP overexpressing RFL-6 cells (16 hrs post-infection) were treated with Tubulin Tracker Deep Red (Thermo Fisher Scientific T34077) and imaged as we have previously described [13] ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Cell Biology 2023Quote: ... Same cDNA was used to amplify both the gene of interest and 18S rRNA in Quant-Studio 6 Flex real-time PCR system (Applied Biosystems). The PCR conditions consisted of AmpliTaq Gold activation at 95°C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets corresponding to two wells of a 6-well plate (double-well pellet with same treatment conditions) were lysed in 1% (v/v) TritonX-100 buffer (Fisher Scientific) in DPBS supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... 3×105 cells were seeded in a 6-well plate and transfected 24h later with 5 µg pX459 plasmid DNA using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... on day -2 fibroblasts were seeded in increasing concentrations on two 6-well plates (10-35 x 104 cells/well) with reprogramming fibroblast medium (PFM; DMEM, 10% ESC-qualified FBS (Thermo Fisher), 1% NEAA (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Molecular Biology 2023Quote: Tumors were engrafted by inoculating 1×106 Nalm-6 cells expressing firefly luciferase and GFP intravenously in 100 μL of PBS (Gibco, USA). Mice were randomly assigned to 2 experimental groups and 1 control group ...
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bioRxiv - Neuroscience 2023Quote: ... BIHi005-A (male) and BIHi250-A (female) were cultured on 6 well-plates coated with hESC-qualified Geltrex (Thermo Fisher, #A1413302) in E8 Medium (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Biophysics 2024Quote: ... The peptides above were redissolved in 25 μL 0.1% TFA and 6 μL of them were analyzed by Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... RIPA Lysis and Extraction Buffer (CAT NO. 89901), and DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) (CAT NO. D3571) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were separated on a 6% precast DNA retardation gel in 0.5% Tris borate-EDTA buffer (TBE, Thermo Fisher Scientific) at 100 V for 1.5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... wash buffers were removed and replaced with a ∼7.6 pH nigericin buffer standard (25 mM HEPES, 80 mM KCl, 1 mM MgCl2) supplemented with 10 μM nigericin (Invitrogen, N1495). Cells were incubated at 37°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... ten-fold serial dilutions from 10-1 to 10-6 of media from infections were prepared in 1 x PBS (Gibco, #14190250). The media on Vero cells seeded the previous day was aspirated ...
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bioRxiv - Evolutionary Biology 2024Quote: ... washing was performed again but cells were resuspended in 12mL of HNM+80μg/mL and plated across a 6-well plate (Thermo Scientific; 140685). Cells were then incubated for 72 hours at 22oC and 60% humidity ...
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bioRxiv - Cancer Biology 2024Quote: The proteomics samples consisting of 24 fractions for the proteome analysis and 6 fractions for the phosphoproteome analysis were analyzed by LC/MS/MS using an Ultimate 3000-Exploris480 (Thermo Scientific). Samples were injected onto an IonOpticks Aurora series 2 C18 column (75 μm id × 15 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... CHLA-42 cells were seeded in 6 well plates and transfected with 25pmol H19 siRNAs according to Lipofectamine ™ RNAiMAX transfection reagent (Invitrogen) manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at 100% density in 6-well plates in NMM medium (50% DMEM/F-12 + GlutaMAX (Thermo Fisher, 31331093), 50% NBM ...
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bioRxiv - Cell Biology 2024Quote: ... Intact spheres were subsequently diluted 1:10 in PBS and placed in a 6 well plate (Fisher Scientific, #07-000-646). Using a standard tissue culture microscope and a 20 uL pipet tip ...
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bioRxiv - Biophysics 2024Quote: ... grids were blotted once for 2.5 – 6 s (4 °C and 100 % humidity) using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) and plunge-frozen in liquid ethane ...
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bioRxiv - Cancer Biology 2024Quote: ... and KiCqStart pre-designed SYBR green gene-specific primers (Supplementary Table S4) in QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Expression level for each gene was normalized to GAPDH for each experiment ...
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: 20 μg of retroviral plasmid DNA (sgGFP-Cr2 or sgZbtb20-Cr3-cs2) with 6 μg pCL-Eco plasmid DNA were transfected using 60 μl Lipofectamine 2000 (Invitrogen, #1668019) in 3 ml OptiMEM (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Scaffolds or 6 mm punches of dissected fresh mouse cortical brain tissue were placed on a Fisherbrand double frosted microscope slide (Fisher Scientific) in the center of an adhesive ...
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bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on glass slides using ProLong Gold antifade (4′,6-diamidino-2-phenylindole) DAPI to counterstain the nuclei (Thermofisher, #P36935).
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bioRxiv - Immunology 2024Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of Gapdh mRNA ...