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Citations for Thermo Fisher :
3701 - 3750 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were blocked in blocking buffer (DPBS containing no Ca2+ or Mg2+ and supplemented with 3% dialyzed FBS; Thermo Fisher). For EC analysis ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Then sections were blocked with a blocking solution (PB supplemented with 0.3% Triton X-100 and 5% normal goat serum, NGS, Gibco, 16210-064) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated with secondary antibody diluted in blocking solution: Goat anti-Rabbit IgG Alexa-488 (Invitrogen A-11008), Goat anti-Rat IgG Alexa 594 (Invitrogen A-11007) ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking was carried out for 15 minutes at room temperature using a blocking solution that included 10 mg/mL UltraPure BSA (Invitrogen AM2616), 0.3% Triton-X ...
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bioRxiv - Systems Biology 2023Quote: ... the cells were blocked and permeabilized for 30 min at RT in a mix of 0.5x PFBB (protein free blocking buffer, Thermo Fisher Scientific) in PBS supplemented with 10 µg/ml tRNA (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed four times in 0.1 M PBS with 0.5% Triton X-100 for 5-10 minutes each and then incubated in blocking solution containing 1:400 secondary antibody donkey Polyclonal anti-rabbit Alexa Fluor Plus 488 (A32790, ThermoFisher Scientific) for 2.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed three times with PBS and incubated for 1 h at room temperature with secondary antibodies diluted in blocking buffer (Alexa fluor (AF) 488 donkey anti-rabbit (1:500; ThermoFisher Scientific), AF568 donkey-anti-rat (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2024Quote: EDTA-containing D-PBS (blocking buffer)) and a goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4°C: AT8 (Mouse, 1:100, Thermo Scientific), and Iba1 (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in blocking buffer (3X SSC supplemented with 2% BSA, 0.1% Triton X100 and 2U/ul RNAse inhibitor (RiboLock, Thermofisher, Cat# EO0384)) ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and then transferred onto nitrocellulose membranes which were blocked with Protein-Free Blocking Buffer (PFBS; Thermo Scientific) for 2 hours at room temperature in agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Per sample 80 µl of Protein G DynabeadsTM were 2 times washed with EBC lysis buffer and blocked overnight at 4 °C under constant rotation with SEA BLOCK blocking buffer (Thermo Fisher). After overnight incubation ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific] ...
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bioRxiv - Bioengineering 2024Quote: ... and then incubated with the secondary antibodies donkey anti-goat IgG Alexa Fluor™ 647 (1:500 in PBS blocking solution, A-21447, Invitrogen) and donkey anti-rabbit IgG Alexa Fluor™ 488 (1:400 in PBS with 0.05% tween ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for one hour at room temperature with a blocking reagent 2% bovine serum albumin (BSA; Fisher Scientific) in PBS followed by staining with primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 4 times with PBS and then incubated with secondary antibody (goat α-mouse AlexaFluor 488 diluted 1:100 in blocking solution, Invitrogen) in the presence of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated overnight in a blocking buffer and primary antibody solutions containing the following: mouse anti-IBA1 (Invitrogen, 1:250), rabbit anti-IBA1 (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... We first incubated whole pre-fixed tissue in 1ml blocking solution (ice-cold PBS solution with 10% (v/v) Heat Inactivated Sheep Serum (HISS) (ThermoFisher, 16070096) and 0.5% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Liquid Chromatography (LC) separation of the peptides was performed on an Ultimate 3000 Dionex RSLC-nano UHPLC (Thermo Fisher Scientific), equilibrated with 95% solvent A and 5% solvent B (equilibration solution) ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides were introduced using an EASY-Spray source at 2000 V to a Fusion Tribrid Orbitrap mass spectrometer (Thermo Scientific). The ion transfer tube temperature was set to 275 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 3000 ng of the mixture tryptic peptides were analyzed using an Ultimate3000 high-performance liquid chromatography system (Thermo Fisher Scientific) coupled on-line to a Q Exactive HF-x mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were digested overnight at 37°C with trypsin (Pierce #90058) and the next day peptides were labelled with the TMTpro16plex reagents according to manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Peptides were trapped on a 2 cm column (Pepmap 100, 3μM particle size, 100 Å pore size; ThermoFisher Scientific, 164946), and separated on a 25cm EASYspray analytical column (75μM ID ...
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bioRxiv - Cell Biology 2020Quote: ... Obtained peptides were separated on a Thermo Scientific™ EASY-nLC 1000 HPLC system (Thermo Fisher Scientific™, Odense, Denmark) using a 45 min gradient from 5-60% acetonitrile with 0.1% formic acid and directly sprayed via a nano-electrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... Peptides were loaded on a C18 PepMap100 pre-column (inner diameter 300μm×5mm, 3 μm C18 beads; Thermo Fisher Scientific) and separated on a 50 cm reversed-phase C18 column (inner diameter 75 μm ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Genetics 2021Quote: Peptides were resuspended in 0.1% TFA and then analyzed using an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an EASY-nLC 1200 system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... lymph-node derived cells were mixed in a 1:2 ratio with OVA-peptide pulsed spleenocytes and cultured in RPMI 1640 culture medium (Gibco) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: The eluted peptides were sprayed into the mass spectrometer by means of an Easy-Spray source (Thermo Fisher Scientific Inc.). All m/z values of eluting peptide ions were measured in an Orbitrap mass analyser ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Microbiology 2021Quote: ... The application of a 2.2 kV distal voltage electrosprayed the eluting peptides directly into the Thermo Exploris480 mass spectrometer equipped with an EASY-Spray source (ThermoFisher). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... 5mM for 10−6M peptide loaded targets and 0.5mM for non-loaded negative control) and ENV641 targets were labeled with CFSE (ThermoFisher Sci) 5mM for 10−6M peptide loaded targets and 0.5mM for non-loaded negative control ...
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Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor controlbioRxiv - Neuroscience 2021Quote: Mouse β4-WT (KKLITFILKKTREK) and β4-Scr (KIKIRFKTKTLELK) peptides (as previously described in Grieco et al. 200551) were synthesized by Thermofisher scientific at 98% purity and diluted to 100 μm in internal solutions for CC and VC experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Molecular Biology 2020Quote: ... Eluting peptides were ionized at a potential of +2 kVa into a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Intact masses were measured from m/z 350-1400 at resolution 120.000 (at m/z 200 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted peptides were directly detected with an ion trap mass spectrometer (QExactive HF; Thermo Fisher Scientific Inc., Waltham, MA, USA). For ionization ...
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bioRxiv - Neuroscience 2020Quote: ... Percolator False Discovery Rate was set to 1% and Peptide abundance values were normalized in Proteome Discover 2.2 (Thermo Scientific). The experiments were performed in two separate sets consisting of (1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluting peptides were analyzed on a QExactive Plus (Qme IP) or QExactive HF (Nhp2 IP) mass spectrometer (Thermo Fisher Scientific). Peptide precursor m/z measurements were carried out at 70000 (Qme IP ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were injected and eluted through a 50 cm EASY-Spray column PepMap RSLC C18 (Cat. ES803; Thermo Fisher Scientific) with a 135-min gradient at constant 300 nL/min kept at 40°C ...
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bioRxiv - Immunology 2022Quote: ... Reconstituted peptides were tested for endotoxin contamination using a Limulus Amebocyte Lysate Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, UK #88282).