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Citations for Thermo Fisher :
3651 - 3700 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA (BSA Fraction V 7.5%, Gibco, cat.# 15260-037) + 5% goat serum (Jackson Immunoresrearch/ Dianova ...
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bioRxiv - Genomics 2022Quote: Samples were then blocked for 1 hour at room temperature in Blocking Solution (1x PBST, 10% Molecular-grade BSA (ThermoFisher AM2616)) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% fat-free skim milk in TBST (Tris buffer saline with 0.05% Tween 20) or SUPERBLOCK T20 (TBS) Blocking Buffer (Thermo Fisher Scientific) then incubated with appropriate primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Triton-X in PBS for three minutes followed by blocking with blocking buffer (1% w/v bovine serum albumin with 0.2% w/v keyhole limpet hemocyanin in PBS; Thermo Fisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Microbiology 2022Quote: ... The primary anti-body was washed away in PBS containing 1% DMSO (wash buffer) and larvae were incubated in blocking solution containing Goat anti-Rabbit546 (ThermoFisher #A11010) at 1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed twice in blocking buffer and twice in TBS before mounting with ProLong™ Glass Antifade Mountant (Invitrogen P36982). Samples were visualized with a Zeiss LSM 710 META inverted laser scanning confocal microscope in part through the use of the Vanderbilt Cell Imaging Shared Resource ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was washed in blocking solution and treated with secondary antibody (anti-guinea pig 488 1:50 and anti-chicken 594 1:300, Invitrogen, WA316328) for 2-3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed 3 times in cold PBS and blocked in FISH blocking buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Cell Biology 2024Quote: ... they were rinsed three times in PBS and blocked for 30 minutes in blocking solution (PBS + 5% by volume normal goat serum, ThermoFisher PCN5000). Staining was for 1 hour with 1:500 anti-OSBP (Atlas Antibodies HPA039227 ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 72-hours in the same blocking solution with anti-FR (1:500, Invitrogen #A6397, made in rabbit) and either anti-TH (1:5K ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked with PLA blocking buffer and incubated with anti-NOD2 antibody (#MA1-16611, ThermoFisher, 1:500: RRID: AB_568643), anti-α-syn aggregate antibody (#ab209538 ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: The blocking solution for all immunostaining approaches was 1X PBS (no Ca2+ / Mg2+) with 1% normal donkey serum (Fisher Scientific; OB003001), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep antibodies were incubated for 1h and after thorough washing and blocking with 5% normal goat serum (Life Technologies) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with PBS and incubated with Alexa Fluor-488 conjugated goat anti-mouse IgG secondary antibody diluted at 1:500 in blocking solution (Invitrogen, USA). Fluorescent foci were visually counted (in a well with a dilution that produced <60 total foci ...
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bioRxiv - Neuroscience 2022Quote: ... then processed for 48 hours at 4 degrees with secondary antibodies in blocking solution (1:1000, Alexa Fluor 647 goat anti-rabbit, Jackson Laboratories, plus 1:500 NeuroTrace, Invitrogen). Tissue sections were then rinsed again 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2023Quote: Immunohistochemistry was performed as follows: brain sections were incubated in a blocking solution consisting of 5% normal goat serum (Thermo Fisher) and 0.1% Triton (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were diluted with odyssey blocking buffer in tris-buffered-saline containing 0.1% Tween20 (TBS-T) (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The lentiviral constructs pHIV-dTomato-CDK19_CasR and pHIV-dTomato-CDK19M_CasR that contain the coding sequences of human CDK19-wild-type or CDK19-D173A kinase-inactive mutant with mutations introduced in the sgRNA-targeting sequence and proximal PAM sequences (blocking sgRNA targeting but not changing the coded protein sequences) were generated using the Phusion Site-Directed Mutagenesis Kit (Thermo Scientific) with the hCDK19-CasR-F/R primers (Table S5 ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Biochemistry 2023Quote: ... UV-crosslinked at 120 mJ and blocked for 2 hours rotating at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Thermo Fisher). Blots were probed rotating overnight at 42°C with 32P-labeled DNA probes prepared as described below ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 in blocking buffer for 1h and counterstained with DAPI prior to mounting with ProLong Gold anti-fade reagent (Invitrogen, P36930) for subsequent immunofluorescence analysis.
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bioRxiv - Genomics 2023Quote: ... Then DNA oligo-conjugated primary antibodies were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized with 0.01% Triton X-100 in PBS for 5 min at room temperature and then treated with BlockAid Blocking Solution (Thermo Fisher) supplemented with FcR Blocking Reagent (Miltenyi ...
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bioRxiv - Microbiology 2023Quote: ... + 0.05% Tween 20 and blocked with 4% milk powder in PBS (Regilait) in the first panning round and Pierce protein free blocking buffer (Thermo Scientific), SEA blocking buffer (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... representative brain sections were wasted three times in 1× PBS and incubated in a 24 well plate at room temperature for one hour in blocking buffer: 10% goat serum (Gibco, 16210064), 1% BSA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... samples were blocked for 15 min in blocking buffer and then incubated with Alexa Fluor conjugated primary antibody against phospho-Src (Tyr419) (44-660G, ThermoFisher Scientific). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for one hour at room temperature with blocking buffer containing secondary antibodies for Pax7 (ThermoFisher #A-11072; 1:500) and MyoD (ThermoFisher #A-11001) ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with peroxidase-linked secondary antibodies for 1h at RT diluted in blocking buffer and then treated with Supersignal West Pico solution (Thermo Scientific). Images were acquired on an LAS-3000 system (Fuji Film).
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody was diluted in blocking solution and added to cells (30 minutes, room temperature) with phalloidin-AlexaFluor568 (1:100 dilution, Invitrogen, A12380) included when indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were treated at 4 °C overnight with the following primary antibodies in blocking buffer: anti-LCP1 (MA5-11921; Thermo Fisher); anti-Vinculin (MAB3574 ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 10 minutes at room temperature and underwent a blocking step with 0.05% (v/v) Triton X-100 and 10% (v/v) normal goat serum (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibody (diluted in the blocking solution) was used: rabbit anti–PGP9.5 (1:400, Thermo Fisher Scientific, #PA5-29012), rabbit anti-S100β (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in blocking buffer containing secondary antibodies (Alexa Fluor 555 donkey anti-rabbit IgG, 1:500 dilution, A31572, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were blocked in blocking buffer (DPBS containing no Ca2+ or Mg2+ and supplemented with 3% dialyzed FBS; Thermo Fisher). For EC analysis ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Then sections were blocked with a blocking solution (PB supplemented with 0.3% Triton X-100 and 5% normal goat serum, NGS, Gibco, 16210-064) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...