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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... was placed at the beginning of the scFc sequence to facilitate protein secretion.54 The plasmid was transfected into HEK 293F cells (Thermo Fisher Scientific). After 88 h of incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in HEK-293T cells using either the Trans-Lentiviral ORF Packaging Kit (Dharmacon/Horizon Discovery) or Lipofectamine 3000 (Thermo Fisher Scientific). Viral supernatants were collected 72 hours (hrs ...
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bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... in the presence of the selection antibiotic when required (HEK-293T-FLAG-CB1R; zeocin at 0.22 mg/mL, Thermo Fisher Scientific #R25001), and were periodically checked for the absence of mycoplasma contamination ...
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bioRxiv - Cell Biology 2022Quote: ... this mixture was added dropwise to confluent HEK-293T cells (ATCC, CRL-3216) in a T175 flask containing 35 mL DMEM media (Gibco #11965-092) and supplemented with 1mM sodium pyruvate (Gibco #11360-070) ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Wild type TGM2 and the TGM2-C277A mutant were overexpressed in HEK 293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in Dulbecco’s minimum essential medium (DMEM/F12; Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Suspension Expi293 cells were grown to 2.5 million cell density and transiently transfected with ADAMs protease expression vectors using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific, # A14525). Medium was harvested after 6-7 days and protein was purified by Ni-NTA chromatography and assessed for quality and activity.
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The PEAK3-1xFLAG construct was transfected into 30 ml of Expi293F culture at a cell density of 4.0 × 106 cells/ml using ExpiFectamine 293 transfection reagent (Thermo Fisher Scientific Cat #A14524) according to the manufacturer’s protocol at 1 μg DNA / 1 ml ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Mutant CRP protein was produced by transfecting 25 µg plasmid DNA into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
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bioRxiv - Synthetic Biology 2023Quote: ... supernatant was harvested from 293S GnTi- TetR sender A’-C (senders 1) and 293S GnTi- TetR sender Γ’-A’ (senders 2) cells that have been continuously cultured in DMEM (41966; Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: Secretion levels from plasmid DNA constructs were evaluated following transient transfections of Expi293F™ cells using the ExpiFectamine™ 293 Transfection kit (Gibco, LifeTechnologies). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... 2×106 HEK-293 cells were electroporated with the pCMV3 vector encoding CD123 wildtype or its variants using the Neon Transfection System (ThermoFisher; 1100V, 20ms, 2pulses). To generate stable cell lines Geneticin G418 (50mg/ml ...
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bioRxiv - Immunology 2023Quote: ... After 5 days the cells were spun down at 80g for 7 minutes at 20°C and media were changed to 30 mL prewarmed Freestyle™ 293 Expression Medium with 0,5% Penicillin-Streptomycin (Thermofisher, cat no: 15140122), vortexed vigorously for 20 seconds to break cell clumps ...
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bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
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bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids listed in Supplementary Table 7 into Expi293F cells was performed according to the manufacturer’s instructions using the Gibco™ ExpiFectamine™ 293 Transfection Kit (Gibco™ A14524). Following transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 cells were suspension cultured in a humidified CO2 Reach-In incubator (37 °C, 5% CO2) using FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific). The cells were grown in vented cap Corning® Erlenmeyer flasks placed on Innova 2100 shaker platforms (New Brunswick Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... Air-liquid interface medium consisted of 50% BEGM and 50% hi-glucose minimal essential medium (DMEM, Gibco) containing 100 nM additional retinoic acid (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus POR1 genomic DNA and inserted into the multiple cloning site (MCS) of pBAD/Myc-His (Invitrogen) vector containing a kanamycin-resistance cassette 25 (in-frame or out-of-frame with the C-terminal Myc-His tag ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Developmental Biology 2021Quote: ... The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat. No. K4800-01, Invitrogen). HEK293T cells were cultured in DMEM medium with 10% FBS in 5% CO2 at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
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bioRxiv - Developmental Biology 2020Quote: ... The template was prepared using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific, UK). Sequencing was performed using the Ion PI Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Bioengineering 2019Quote: ... and cloned into the standard mammalian expression vector pEF1/myc-His B (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Cell Biology 2020Quote: ... The protease was removed using His-Pur™ Ni-NTA agarose resin (Thermo Fisher Scientific, Waltham, MA). Gle1152-360 was further purified by a 30% ammonium sulfate precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The stable cell lines were generated using the pcDNA/FRT/V5-His TOPO TA Expression kit (Invitrogen) performed according to the manufacturer’s protocol and as described previously (28) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Microbiology 2021Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in Express-Five media (Life Technologies) and infected with 0,5% vol/vol of generation 2 (V1 ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Molecular Biology 2020Quote: The sequence of the OpIE2 promoter was obtained from pIB/v5-His map sequence (ThermoFisher Scientific, USA). The truncated version of the wild-type OpIE2 promoter sequence (tIE2) ...
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bioRxiv - Microbiology 2020Quote: ... The templates were sequenced with an Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific Inc.) and a 318 Chip Kit v2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Biochemistry 2022Quote: ... and AcGFP1 DNA fragments were synthesized (Gene Universal) and inserted into pAc5.1/V5-His A vector (Invitrogen). The FUS-Venus plasmid was a kind gift from Dr ...
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bioRxiv - Microbiology 2021Quote: ... then cloned into the BglII and XhoI sites of the pMT/BiP/V5-His vector (Invitrogen, #V413020). The recombinant protein was expressed and purified using the Drosophila Expression System as described previously (Schuijt et al. ...
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bioRxiv - Microbiology 2021Quote: ... purified His-RBD was diluted to 200 μg/mL in PBS buffer containing Sypro Orange 5× (ThermoFisher) in a 96-well white PCR plate ...