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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... pHrodo (Invitrogen, P36600, 3 μM) dissolved in DMSO was added to the synaptosomes and incubated for 45 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... DiIC12(3) (Thermo Fisher Scientific), was added to a final concentration of 5 μg/mL in GPMV buffer and incubated for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: HEK 293T (ATCC, Manassas VA, CRL-3216) cells were grown at 37°C with 5% CO2 in DMEM (Gibco, Waltham MA, #11965-092), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Genetics 2022Quote: Human embryonic kidney cell line (HEK 293T) was purchased from the American Type Culture Collection and cultured in DMEM medium (GIBCO, Cat. NO. 12430) supplemented with 10% FBS (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Equal ratios of heavy and light chain plasmids were co-transfected into HEK 293F cells using FreeStyle MAX (Thermo Fisher Scientific, catalog #16447100) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells were purchased from ATCC and cultured in high glucose DMEM with 2 mM GlutaMAX and pyruvate (Life Technologies catalog #10567022) supplemented with 10% heat-inactivated characterized fetal bovine serum (Hyclone ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293T cells stored at -80°C in freezing medium (DMEM high glucose, Sigma-Aldrich, supplemented with 20% FBS, Gibco, and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... RPE1 and HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose, GlutaMAX™ and pyruvate supplemented (ThermoFisher Scientific, Waltham, MA, USA)) + 10% Fetal Calf Serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfection was performed using expression medium comprised of a 9:1 ratio of Freestyle™293 expression medium (Thermo Fisher Scientific, Waltham MA) and EX-Cell expression medium including Glutmax (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... A 1 mL stock of 1*10^7 cells were rapidly thawed from −80°C in 37°C water bath and transferred to 19 mL prewarmed Freestyle™ 293 Expression Medium (Thermofisher, cat no: 12338018) and incubated at 37°C at 8% CO2 under orbital shaking at 125 rpm in a 125 mL Erlenmeyer flask ...
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bioRxiv - Biochemistry 2024Quote: ... A Flp-In 293 cell line containing a single stably integrated FRT site at a transcriptionally active genomic locus was purchased from Invitrogen (R75007, Thermo Fisher Scientific). The FDA approved drug library was from APExBIO (L1021 ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... Cells were cultured in DMEM (Wisent) or RPMI1640 supplemented with 10% FCS and penicillin/streptomycin (Gibco). Media were supplemented with 200 UI/mL IL-2 (Proleukin – Novartis Pharmaceuticals ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% (v/v) heat-inactivated Hyclone fetal calf serum (FCS; Thermo Scientific, UT, USA), 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cells were then subjected to Fc blockade with anti-CD16/CD32 (Thermo Fisher Scientific, Waltham, MA), washed with 1XPBS ...
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bioRxiv - Immunology 2022Quote: ... The isolated lungs were placed in RPMI-1640 containing 2% (vol/vol) FCS (Thermo Fisher Scientific), 50 μg/ml Liberace TM (Roche ...
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bioRxiv - Immunology 2022Quote: ... explanted organs were stored in 600 μL DMEM containing 10% FCS and 1% Penicillin/Streptomycin (Gibco). After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% (vol/vol) fetal calf serum (FCS, Biochrom) and antibiotics (1x Pen/Strep, Gibco). tRF were generated from primary rhesus fibroblasts (RF ...
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bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were cultivated in DMEM-F12 medium containing 20% fetal bovine serum (FCS, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... hDPP4-Fc and G2 were biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific) and 80% maximum binding concentrations against S-2P were determined with biotinylated proteins (hDPP4-Fc-biot and G2-biot) ...