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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... 3 μm (Thermo-Fisher Scientific) at 10 μL/min ...
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bioRxiv - Molecular Biology 2023Quote: ... 3% TFA (Thermo Scientific, 28903)) ...
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bioRxiv - Immunology 2023Quote: ... or TO-PRO-3 (ThermoFisher). Samples were mounted using ProLong Diamond Antifade Mountant and curated for 24 hours before imaging ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL 0.1% BSA (Ambion), 3 µL 10x reaction buffer (NEB) ...
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bioRxiv - Immunology 2023Quote: ... 3 mM L-glutamine (Invitrogen), non-essential amino acids (NEAA ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (Thermofisher: AM9530G); and 0.1% NP-40 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for Qubit 3 fluorometer (Invitrogen) and 100 / 30 ng (immunoprecipitation/input ...
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bioRxiv - Biochemistry 2024Quote: ... DiIC12(3) (Thermo Fisher Scientific), was added to a final concentration of 5 μg/mL in GPMV buffer and incubated for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Step 3: Cavicid (Fisher Scientific), Step 4 ...
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bioRxiv - Cell Biology 2024Quote: ... Flt-3 (A2F10, Thermofisher Scientific), CD48 (HM48-1 ...
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bioRxiv - Neuroscience 2024Quote: ... pHrodo (Invitrogen, P36600, 3 μM) dissolved in DMSO was added to the synaptosomes and incubated for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml Puromycin (Gibco) was added 48 hours after transduction before knockdown analysed >8 days post transduction via qPCR and western blot if appropriate32.
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml puromycin (Gibco) was added 48 hours post-transduction ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl Lipofectamine RNAiMAX (Invitrogen) was mixed with 100 μl Opti-MEM and incubated at room temperature for 1 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 3% bovine serum albumin (ThermoFisher) and 5% normal goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 3 µm column (Thermo Scientific) and a 6210 electrochemical cell (ESA ...
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bioRxiv - Cell Biology 2024Quote: ... with Quantstudio 3 (Applied biosystems). microRNA levels were normalized by as the combination of endogenous control as internal standard ...
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bioRxiv - Cancer Biology 2024Quote: ... and QuantStudio 3 (Thermo Fisher). All primers used here are listed in Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... while the rest were re-plated onto three matrigel-coated 10 cm dishes (3 replicates / 3 million cells per plate) in N2/B27 media containing 3 µg/mL puromycin (Fisher Scientific; Cat. no. 501532829) to select for the pMK1334 sgRNA vector ...
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bioRxiv - Biochemistry 2020Quote: HEK 293T (ATCC, Manassas VA, CRL-3216) cells were grown at 37°C with 5% CO2 in DMEM (Gibco, Waltham MA, #11965-092), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Genetics 2022Quote: Human embryonic kidney cell line (HEK 293T) was purchased from the American Type Culture Collection and cultured in DMEM medium (GIBCO, Cat. NO. 12430) supplemented with 10% FBS (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Equal ratios of heavy and light chain plasmids were co-transfected into HEK 293F cells using FreeStyle MAX (Thermo Fisher Scientific, catalog #16447100) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells were purchased from ATCC and cultured in high glucose DMEM with 2 mM GlutaMAX and pyruvate (Life Technologies catalog #10567022) supplemented with 10% heat-inactivated characterized fetal bovine serum (Hyclone ...
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bioRxiv - Cancer Biology 2024Quote: ... RPE1 and HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose, GlutaMAX™ and pyruvate supplemented (ThermoFisher Scientific, Waltham, MA, USA)) + 10% Fetal Calf Serum (FCS ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293T cells stored at -80°C in freezing medium (DMEM high glucose, Sigma-Aldrich, supplemented with 20% FBS, Gibco, and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Immunology 2024Quote: ... equimolar quantities of each RNAs (equivalent to 100 ng) were transfected to HEK-293T cells per well in 96-well plate using Lipofectmine 3000 (Thermo Fisher Scientific, L3000001) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Human embryonic kidney (HEK) 293T cells were maintained at 37°C in 5% (v/v) CO2 in complete medium (DMEM medium (Thermo Fisher Scientific, #31966047), 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... stably CD95L-IZ expressing HEK293T cell pools were grown to a density of 1 × 106 cells / mL in HEK FreeStyle293 medium (Gibco, Thermo Fisher Scientific), gene expression was induced using 1 µg/mL doxycycline and culture supernatant was harvested three days post-transfection.
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...