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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2023Quote: ... IgGs were isolated from sera using 2 mL CaptureSelect IgG-Fc resin slurry (Thermo Scientific) per 1 mL rabbit sera ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociation was stopped by addition of 2 volumes of DMEM-F12 with 10% FCS (GIBCO) and cells were counted ...
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bioRxiv - Genetics 2024Quote: ... We measured the absorbance at 492 nm in a microplate reader (Thermo Scientific Multiskan FC). Plotting of graphs and statistical analyses were conducted with GraphPad Prism 8.4.2.
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed every hour using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 620 nm.
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Alexa Fluor 488 Anti-alpha 1 Sodium Potassium ATPase antibody (ab197496) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS and 1 U/mL Penicillin-Streptomycin (10 000 U/mL, Gibco) in 96 well round-bottom plates ...
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bioRxiv - Cell Biology 2023Quote: ... MDA-MB-231 cells were grown in DMEM/F12 with 10% FCS (Thermo Fisher Scientific) and 100 U μL/mL penicillin and 100 μL/mL streptomycin ...
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bioRxiv - Immunology 2023Quote: TAP2 deficient CHO cells were cultured in complete RPMI (Invitrogen, 2mM glutamine and 10% FCS) and stably-transfected with >10g plasmid DNA using poly-L-ornithine and a 30% DMSO shock ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance was determined at 450 nm using a microplate reader (Thermo Scientific Multiskan FC). All experiments were carried out in four biological replicates.
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were treated with anti-CD16/32 (Fc block) (14-9161-73, ThermoFisher) and stained with the live/dead marker ethidium monoazide bromide (ThermoFisher #E1374 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (590nm excitation and 620nm emission) with Multiskan FC (Thermo Scientific) [33 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (544nm excitation and 590nm emission) with Multiskan FC (Thermo Scientific) [31].
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% L-glutamine (Gibco™ 25030-024). The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30 ...
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bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
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bioRxiv - Cancer Biology 2023Quote: ... The data was immediately collected by Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, USA) at 450 nm ...
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bioRxiv - Plant Biology 2023Quote: ... Absorbance at 340 nm was monitored continuously with a spectrophotometer (MULTISKAN FC; Thermo Fisher Scientific) to detect NADH production.
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039 ...
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bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
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bioRxiv - Physiology 2023Quote: ... and blocked on ice for 20 minutes with Fc Block anti-CD16/CD32 (Thermo Fisher). Then ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco). Huh 7.5 cells were maintained in complete DMEM medium supplemented with non-essential amino acids.
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... OD600 measurement in a microplate reader (Multiskan™ FC, Thermo Scientific, Etten-Leur, the Netherlands) where performed ...
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bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
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bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Fc receptors were then blocked with anti-mouse CD16/CD32 (Invitrogen, Cat# 14-01061-86) along with extracellular staining with a mixture of conjugated antibodies in FACS buffer (1:250 for LP cells ...
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) horseradish peroxidase (HRP) was purchased from Invitrogen and 3,3″,5,5″-Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
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bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
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bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
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bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
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bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies with HRP (ab97250 ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies, Carlsbad, California, USA) was used according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293T/17 cells were purchased from ATCC (Cat. No. CRL-11268) were maintained in DMEM (Thermo Fisher Scientific, Cat. No. MT10013CV) with 10% fetal bovine serum and 1% Pen-Strep ...
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bioRxiv - Immunology 2020Quote: ... The purified plasmid DNA (both heavy and light chain) was transfected into a 30mL culture of HEK Expi293 cells (Thermo Scientific Expi293). Harvesting the culture after 5 days ...
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bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: The two lentiviral particles of the barcode and sgRNA vectors were individually transfected into HEK-293FT cells (Cyagen, no. HEKFT-30001) with lentivirus packaging plasmids using Lipofectamine 2000 (ThermoFisher, no. 11668027). To obtain the viral particles ...
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bioRxiv - Genomics 2022Quote: ... HEK-293T cells were grown on a 10 cm dish up to 70-80% confluency in HEK-media (KO-DMEM (Themro Fisher Scientific, 10829018), 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Systems Biology 2024Quote: ... VP4:VP2-complemented CVB3 A67G was prepared by transfecting HEK-293T cells with equal molar ratios of pcDNA CVB3 A67G and pcDNA3 VP4:VP2 using Lipofectamine 2000 (Thermo Fisher, 11668019). Viral supernatants were concentrated by ultracentrifugation (100,000 rcf for one hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: HEK-293T (CRL-11268) cells and PK-15cells (CCL-33) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Carlsbad, CA USA) supplemented with 10% fetal bovine serum (FBS ...