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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Hoechst 43222 (H1399) and ProLong Gold Antifade Mountant with 4′,6- diamidino-2-phenylindole (DAPI; P36931) were purchased from Invitrogen.
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064; 10 % horse serum, Gibco 26050-088 ...
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bioRxiv - Cell Biology 2022Quote: ... The plates were then washed with PBS 3×10min at RT on a shaking plate and cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064 ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was completed using the ThermoFisher Taqman Gene Expression Assays according to manufacturer’s protocol in the QuantStudio 6 Flex System (Applied Biosystems) mRNA targets included ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using the iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5% non-fat milk in TBS containing 0.1% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using SYBR Select Master Mix and QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with the following parameter ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed with 1xPBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306). Coverslips were then mounted in Vectashield (LSBio ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Cell Biology 2022Quote: PBMCs were thawed in a 37°C water bath and plated into a 6-well plate (353046, Falcon) with RPMI media (11875-093, Gibco), supplemented with 10% fetal bovine serum (900-108 ...
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bioRxiv - Immunology 2022Quote: Bone marrow was harvested from the long bones of C57BL/6 mice by flushing with Hank’s Balanced Salt Solution (HBSS, Gibco, UK). Cells were cultured overnight in DMEM containing 10% fetal bovine serum ...
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bioRxiv - Genetics 2022Quote: ... and were seeded at 106 cells per well in neural expansion medium (NEM) in 6 well plates coated with Geltrex (A1413201, Gibco). This was considered as Passage 0 (P0) ...
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bioRxiv - Microbiology 2022Quote: Monocytes were isolated from the femurs of 6-12 week-old male C57BL/6 mice and differentiated into BMDMs for six days in 70% v/v RPMI 1640 medium (ATCC modification) (Gibco), 20% v/v L929 cell conditioned medium (provided by the Cell Services Science Technology Platform at the Francis Crick Institute) ...
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bioRxiv - Physiology 2022Quote: ... The FDB was rapidly dissected free and pinned to the bottom of a sylgard-coated dish containing collagenase type II (6 mg/ml, Gibco) in standard Tyrode’s solution ...
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bioRxiv - Neuroscience 2022Quote: ... qPCRs were run using PowerUp SYBR green master mix on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). Mouse primers used for qPCR:
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bioRxiv - Microbiology 2022Quote: ... The grids were blotted for 6 s and plunged into liquid ethane using a vitrification apparatus (Vitrobot, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: LF82 infected and control cells were lysed at 6 hours post-infection (hpi) for 15 min in radioimmunoprecipitation assay (RIPA) lysis buffer (ThermoFisher) supplemented with protease inhibitor (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed on an ABI Quant Studio™ 6 Flex system (Applied Biosystems, Waltham, MA, USA) with SYBR Green PCR master mix (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... and reactions were performed in eight-link boards on QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, USA) with cycling conditions (50 °C 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fugene HD transfection reagent and appropriate volume of Opti-MEM Reduced Serum Medium (31985070, Thermo Fisher, MA, USA) was prepared and incubated for 15 min at RT ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Microbiology 2022Quote: ... and P323L/G671S N-terminally tagged with 6×His were purified using Bac-to-Bac™ Baculovirus Expression System based on the manufacturer’s instruction (Gibco). Upon 3 dpi of NSP12-enconding baculoviruses into Sf9 cells ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed in a 96-well plate using an Applied Biosystems QuantStudio 6 RT-PCR system (ThermoFisher Scientific). Reactions of 50 μLs in total volume were used which contained ...
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bioRxiv - Neuroscience 2023Quote: Cultured neurons were incubated for 1 min with fluorescent dye N-(3-triethylammonium-propyl)-4-(6-(4-diethylamino)phenyl)-hexatrienyl)pyridinium dibromide (FM4-64; 4 µM; Invitrogen) and loaded by stimulation (10 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... Neuronal differentiations were carried out by plating 200,000 cells/12 well-well or 500,000 cells/6 well-well in DMEM/F12 base media (Gibco 11320-033) supplemented with B27 (Gibco 17504-044) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in a flow-cytometry buffer and DAPI (4′,6-diamidino-2-phenylindole, 0.5 μg/ml; ThermoFisher #D1306) solution to exclude dead cells ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed 6 times for 20 minutes and incubated in secondary antibody (Alexa Fluor 488 goat anti-chicken-488, #A11039, Invitrogen) diluted 1:200 in 10% normal goat serum for 3 hours in the dark at room temperature ...
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bioRxiv - Immunology 2022Quote: ... primers are listed in Supplemental Table 3), Luminex using a custom procarta Luminex 6-plax-cytokines (IL1β, IL6, IL8, IL18, CCL2, and TNFa) detection kit (ThermoFisher) (Supplemental Fig ...
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bioRxiv - Genomics 2022Quote: ... For each transfection 1.6 ug of plasmid DNA has been incubated for 20 minutes with 4 µl of Lipofectamine 2000 transfection reagent (Invitrogen) in 0.2 ml of OptiMEM media (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were reconstituted in 6 uL 0.1% formic acid (FA) 0.05% heptafluorobutyric acid (HFBA) (Thermo Fisher Scientific, cat. 25003).
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed in 4% formaldehyde at room temperature for 10 minutes and mounted onto slides using ProLong Gold or Diamond Antifade Mountant with 4′,6-diamidino-2- phenylindole (DAPI) (ThermoFisher). Images were obtained using a Leica SP8 confocal microscope using excitation/emission spectra ...
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were plated at 1 x 106 cells/well in a 6-well plate and cultured for 24 hr in 2 ml of F12 medium (Gibco) supplemented with 10% FBS and 1% Glutamax (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... in a final volume of 12.5 μL per reaction in 384-wells PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...
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bioRxiv - Bioengineering 2023Quote: ... Transfer to a nitrocellulose membrane was done using 25 V for 6 min in the iBlot2 Gel Transfer device (IB21001, ThermoFisher). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... and DU145 cells were plated at 500 K cells per well on 6-well plates (Thermo Scientific™, Cat# 140675) coated with 10 µg/mL poly-D-lysine (PDL ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were performed at 30°C for 10 min and terminated by addition of 0.4% SDS and 6 × loading buffer (Thermo Scientific). The reaction mixture was loaded onto an 0.8% agarose gel in 1x TAE (40 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: Doxycycline-inducible C2C12 cells were seeded for most treatments at 0.1X106 cells per well in 6-well plates with 2 ml of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA fragments in pENTR-D-TOPO were transferred to the modified pHAGE plasmids (6) using LR recombination (Invitrogen 56484).
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA for PCR amplification was obtained by reverse transcription of RNA extracted from 6 dpf embryos with TRIzol (15596026, Invitrogen) using the QuantiTect Reverse Transcription kit (205311 ...
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bioRxiv - Cancer Biology 2024Quote: 2×106 cells were seeded in 6 well plates and were incubated 24 hours later with 1uM of CellTrace Violet reagent (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... fowleri trophozoites were grown to near 100% confluency in 6-well TC-treated plates (Thermo Scientific Nunclon Delta Surface 140685). HEX (25 µM ...
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bioRxiv - Biochemistry 2024Quote: Bacmids containing 6×His-MBP-TEV-nsp12 variants were generated using the Bac-to-Bac™ baculovirus expression system (Invitrogen). Sf9 cells were transfected with the bacmid DNA and baculovirus-containing cell supernatants were harvested 5 days after transfection ...
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...