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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2019Quote: A total of 8 µg of plasmid DNA was transfected into semi-confluent HEK293FT cells on 6-cm-diamitor culture dishes using Lipofectamine 2000 (Invitrogen). Epoxomicin or DMSO was added to the culture 4 h before lysate preparation ...
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bioRxiv - Neuroscience 2019Quote: ... Larvae were placed in a 6 cm Petri-dish containing a mixture of 0.2 M sucrose and 0.08% Brilliant Blue R dye (Acros Organics, #191490050). The larvae were allowed to feed on the sugar solution for 15 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Assays were conducted at 25 °C in 0.5 mL containing 50 mM MES (pH 6) and 80 mg/mL soluble starch (Acros Organics). Some assays solution also contained 100 mM KCl ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using PerfeCTa SYBR Green SuperMix ...
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bioRxiv - Cell Biology 2019Quote: 293T cells in 6-well plates at ∼80% confluency were transfected with 3µg plasmid encoding eYFP using TurboFect transfection reagent (Thermo Fisher). 24h post transfection ...
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bioRxiv - Molecular Biology 2019Quote: Cells from one well of a 6-well plate were spun down and resuspended in 1mL TRIzol® (Life Technologies) for each respective sample ...
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bioRxiv - Microbiology 2019Quote: Huh7 cells seeded at 5×105 in a 6 cm cell culture dish were transfected 24 hours later with the indicated siRNA duplex using Lipofectamine 3000 (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: 22RV1 cells were seeded in 6-well plates and transfected in solution with 20nM miRVana mimic (miR-194 or negative control; Ambion) using RNAiMAX (Life Technoloies) ...
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bioRxiv - Bioengineering 2019Quote: ... 500 ng of each Acr vector and GFP plasmid used as a transfection control were transfected in a 6-well format using Lipofectamine 2000 (Invitrogen). The total DNA amount was kept constant by adding a stuffer plasmid in all cases.
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bioRxiv - Physiology 2020Quote: ... Measurements of mRNA were performed with SYBR green chemistry using the QuantStudio 6-flex Real-time PCR System (ThermoFisher Scientific). All data were normalized to either Hprt or Actinb by the ΔC(t ...
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bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
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bioRxiv - Molecular Biology 2019Quote: For depletion experiments HeLa cells were transfected in 6-well plates at 60-80% confluency using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher, #11668019). Puromycin (conc ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... mixtures were subjected to 8% SDS-PAGE with 6 µl of molecular weight marker (SeeBlue Plus2 Pre-Stained Standard, Invitrogen), after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane ...
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bioRxiv - Microbiology 2021Quote: Total RNA was also extracted from ovine abomasum and ileum tissue harvested at post-mortem from five individual 6-month old helminth-free Texel cross lambs and stored in RNAlater (ThermoFisher). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was performed by using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA) with cDNA as the template ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Cell Biology 2020Quote: ... Seoul National University) were seeded into 6-well culture plates using DMEM supplemented with 10% newborn bovine serum (Thermo Fisher), and 1% penicillin/streptomycin at a density of 1 × 105 cells/well ...
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bioRxiv - Bioengineering 2020Quote: ... The CdWO4 scintillator plates were taken out from the 6-well plates and mounted onto a standard glass coverslip (0.1 mm thick, Fisher Scientific) upside down ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Bioengineering 2020Quote: ... A wad punch was used to create discs that fit snuggly into Nunc™ 6-well plates (Thermo Fisher Scientific). Individual microwells measured 2 mm x 2 mm with a depth of 0.8 mm [3] ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were cultured in a 6-well plate with the DMEM medium (Thermo) and 10% FBS (Gibco, cat# 10091148) to generate the lentivirus ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature in a holding chamber containing oxygenated imaging media (50% MEM, 50% HBSS, 6 mg/mL D-glucose, and 25 mM HEPES; all from Invitrogen/ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... Then animals were placed in the middle of a 6 cm diameter petri dish lid and embedded in 0.8% low melting point agarose (Thermo Fisher). To bypass the blood brain barrier and permit for acute pharmacological intervention ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Neuroscience 2020Quote: ... Transfection was carried out in unsupplemented NBA using 500 ng of the luciferase reporter construct and 20 ng of a normalizer plasmid pGL4.83-mPGK-hRLuc at 5-6 DIV using Lipofectamine 2000 (Thermo Scientific) with DNA to Lipofectamine ratio of 1:2 ...
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bioRxiv - Neuroscience 2020Quote: ... After electroporation the cells were immediately plated at low density onto vitronectin-plated 6 well plates containing warm Stemflex media supplemented with ROCK inhibitor (RevitaCell, Gibco). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: 1.5×104 cells were seeded in one well of a 6-well plate (Falcon) with growth medium comprising of DMEM/F-12 (Gibco) with 20% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection was performed overnight on attached cells growing in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 10μL of 20μM siRNA per well ...
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bioRxiv - Cancer Biology 2020Quote: A549/KZFP cells and NC cells were seeded at 1.0 × 105 cells/well in 6-well plates (Thermo Fisher Scientific). After 72 hours of seeding ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of mitochondrial superoxide was performed in acute brain slices (400 μm) from 6 month-old mice by incubation with 5μM MitoSOX Red (Life Technologies) in ACSF for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... equal amounts of RNA were prepared in equal volumes (6 μL) using UltraPure DNase/RNase-free distilled water (Invitrogen™). RNA samples were denatured at 95°C for 3 minutes and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Cell Biology 2021Quote: qPCR analysis was performed using the LabTaq Green Hi Rox (Labtech) following manufacturer’s instructions on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) and primers listed in Supplementary Table S4.
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bioRxiv - Microbiology 2020Quote: 1 μg of total RNA from mock- and ZIKV-infected cells was resuspended in 2x formamide RNA loading buffer and run on a 6% denaturing PAGE gel (Invitrogen) with an RNA ladder (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Microbiology 2020Quote: ... Organoids in expansion media grew in culture for 4-6 months and needed to be routinely passaged with TRYPLE (Gibco). To induce differentiation to a hepatocyte-like fate ...
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bioRxiv - Plant Biology 2020Quote: ... qPCR reactions were performed as described in (Ramirez-Garcés et al., 2016) and conducted on a QuantStudio 6 (Applied Biosystems) device using the following conditions ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...