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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Day 6 moDCs were transfected with GFP-WT cofilin or mCherry-labelled mutant cofilin using a Neon electroporator (Thermofisher Scientific). moDCs were pulsed twice at 1000 V for 40 ms and using 10 µg of the corresponding plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... Pax9 was cloned from 24 hpf cDNA using the primers pax9F 5’-TCTAGAATGGAGCCAGCCTTT-3’ and pax9R 5’-ATGGATCCTCATAGAGCTGAAGCCACCAG-3’ (Supplementary Table 6) and cloned by TOPO-TA to the pCRII vector (Invitrogen) to create pCRII pax9 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with CaR buffer containing either 2 mM CaCl2 or 2 mM EDTA in the absence or presence of 6 mM MβCD (Thermo Fisher). For monitoring PS exposure and dextran permeability ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on generated cDNA with SYBR Green using QuantStudio 6 Real-Time PCR (ThermoFisher Scientific, Australia). Forward and reverse prime sequences for each gene of interest are shown in Table 7 ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Genetics 2023Quote: ... surviving cells were passaged on Matrigel-coated 6 well plates at a concentration of 500K cells/well in neural differentiation medium {Neurobasal medium (Gibco), B27 supplement (Gibco) ...
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bioRxiv - Microbiology 2023Quote: Cells incubated in 6-well plates for 48 h following transfection were lysed using 750 μl of TRIzol (Life Technologies). RNA extraction was performed using the miRNeasy® mini kit (QIAGEN ...
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bioRxiv - Immunology 2023Quote: Purified CD4+ naïve T (Tresp) cells from CD45.1+ C57BL/6 mice were stained with CellTrace violet (CTV) (Thermo Fisher Scientific). Tresp cells (5 × 104 ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: Agar plate preparation: 7.5g agar (BD Difco™ Dehydrated Culture Media: Potato Dextrose Agar, Fisher Scientific, Cat# DF0013-17-6) was added to the 500 mL of liquid media ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded onto 0.1% gelatine-coated 6-well plates in DMEM supplemented with 10% foetal calf serum (Gibco, ThermoFischer, #26140079) and 1x Glutamax (Gibco ...
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bioRxiv - Bioengineering 2023Quote: PAAm gels prepared on coverslips were transferred to multiwell plates before covering with 0.2 mg/mL sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH) (Thermo Fisher) solution ...
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bioRxiv - Neuroscience 2023Quote: ... brains were washed trice with PBS for 5 min and free amines were anchored with 0.1mg/mL succinimidyl ester of 6-((Acryloyl)amino)hexanoic acid (Acryloyl-X, SE, Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded onto 6-well or 96-well plates for 24 hours and followed by siRNA (10 nM) transfection using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and 50 ng Myc-tagged GFP constructs were forward transfected into HFT cells seeded in a 6-well plate using Lipofectamine 3000 (Invitrogen). The cells were harvested 48 or 72 hours post transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 January 2014) were thawed from cryovials and added to pre-warmed newborn calf serum (NCS, Gibco, Breda, The Netherlands), and centrifuged at 450g for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Primary cortical neurons were dissociated from brains of E18 Sprague-Dawley rat embryos or E16 C57Bl/6 mouse embryos and cultured in Neurobasal medium (Gibco) as previously described (Gopal et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were plated 24 hours before transfection in a cell-culture treated 6-well plate (Fisher Scientific, 14-832-11). To transfect ...
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bioRxiv - Immunology 2023Quote: ... and a FAM-ZEN/Iowa Black FQ labeled probe sequence (Integrated DNA Technologies) on the QuantStudio 6 system (Applied Biosystems). An Ultramer DNA oligonucleotide spanning the amplicon (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... cranial neural crest induction was initiated (D0) by replacement of the stem cell maintenance media with neural crest induction media A (Essential 6 base medium (Gibco), 600nM CHIR99021 (Biogems) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was centrifuged at 40,000 g and supernatant was loaded onto 6 ml of HisPure Ni-NTA resin (Thermo Scientific). The column was then washed with 7-column bed volumes (CV ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cultured to approximately 60% confluency on 24-or 6-well plate and transfected with siRNAs at final concentrations of 10-25nM using lipofectamine RNAiMAX (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Neonatal HDFs were seeded in 6-well plates and transfected with 500ng of each plasmid per well using Lipofectamine 3000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... CRL-3216] and Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... and randomly distributed on 0.4µm Greiner Thincerts and placed in a 6-well plate containing 2ml DMEM/F12 (Thermo Fisher #31330038) + 10% FCS (Merck Millipore S0615 ...
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bioRxiv - Molecular Biology 2023Quote: The full length TF and corresponding IDR S2 cell lines were expanded to 6-well plates and prepared for imaging by resuspending and transferring to 27 mm glass bottom cell culture dishes (ThermoFisher). Cells were imaged on a Zeiss (Germany ...
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bioRxiv - Genomics 2023Quote: ... The IVT product was mixed with 6 μL ExoSAP-ITTM PCR Product Cleanup Reagent (Thermo Fisher Scientific, Cat. # 78200.200.UL) and incubated at 37°C for 15 mins ...
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were seeded in 6-well plates the day before transfection with the WSN reverse genetics plasmids in Opti-MEM (Gibco) using FuGene®6 (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Vero cells infected with unlabeled HSV-1 or OK15 at 10PFU/cell and were harvested from 6-well plates at the various timepoints and conditions indicated above using 0.3mL Trizol reagent (Life Technologies), and immediately stored at -20⁰C ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Biophysics 2023Quote: The membrane probes Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were obtained from Molecular Probes (Eugene, OR, USA) and dissolved in ethanol and dimethyl sulfoxide (DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT–PCR system (Applied Biosystems). Relative quantities of viral and cellular RNA were calculated using the standard curve method ...
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bioRxiv - Cancer Biology 2023Quote: EMT-6 tumors were analyzed on a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with heated electrospray ionization (HESI ...
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bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated into 6-well plates and transfected at ∼75% confluency with1 μg of DNA using lipofectamine 3000 (ThermoFisher). For imaging ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cancer cell lines with z stable Cas9 overexpression introduced by lentiviral infection were seeded in 6-cm dishes and transfected with gRNA mixes using Lipofectamine MessengerMax (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Immunology 2023Quote: ... The reaction was then rapid cooled down on ice and 6 μL of 5x FS Buffer (Invitrogen, cat. no: 18080044), 1.5 μL of 0.1 M DTT (Invitrogen ...