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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... embedded organoids were mechanically dissociated from Matrigel and transferred to a 6 well-plate on a CO2 resistant orbital shaker (ThermoFisher) and grown in differentiation medium consisting of DMEM:F12 supplemented with 1X N2 supplement ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Neuroscience 2019Quote: ... Neurons were transfected with the CofActor optogenetic system (6 µg plasmid/plate) on day in vitro 3-5 (DIV3-5) using Lipofectamine 2000 (Invitrogen). 48 hours post transfection ...
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bioRxiv - Neuroscience 2019Quote: ... frozen brains from C57BL/6 WT or Prnp0/0 (PrP-KO) mice 43 were gently chopped in few drops of Hibernate-E (Gibco) and transferred to a 15 mL tube containing 75 U/ml of collagenase type III (Worthington ...
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Plant Biology 2021Quote: The MS/MS spectra from each LC-MS/MS run were searched against the proteomes listed in Supplementary Table 6 using the SEQUEST searching engine of Proteome Discoverer (Version 2.1, PD2.1, Thermo Scientific, USA). The search criteria were set as follows ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Cancer Biology 2020Quote: ... or 100 pmol (for each well in 6-well plate) of each siRNA was transfected into abl or DU145 cells using Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg DNA was mixed with 6 µL of Fugene HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at room temperature for 30 min ...
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bioRxiv - Genomics 2021Quote: Organoids seeded in 6-well plates were harvested after 3-days incubation with WYE-132 in RIPA buffer (Thermo Scientific) supplemented with protease inhibitors (Complete Mini ...
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bioRxiv - Cell Biology 2020Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs-derived small molecule neural precursor cells (smNPCs) were cultured on Matrigel-coated 6-well plates and kept in N2B27 medium consisting of DMEM-F12 (Invitrogen)/Neurobasal (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cell Biology 2021Quote: ... Transcript abundance was quantified on a QuantStudio 6 Real Time PCR system using SYBR® Green PCR Master Mix (ThermoFisher) and gene-specific primers (Supplementary Table S5) ...
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bioRxiv - Developmental Biology 2022Quote: ... The 6 μm thickness section was prepared on the Microm HM 340 E rotary microtome (Thermo Scientific, Waltham, MA, USA), and dewaxed in xylene and serial concentrations of ethanol before staining ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell aggregates were transferred into ultra-low attachment 6-well plates in EB medium consisting of 20% knockout serum replacement (Gibco), 79% DMEM/F-12 ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 2.5 µg of plasmid per well of a 6-well plate using Lipofectamine 3000 (Invitrogen®) and protein expression levels analyzed 24h after transfection.
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bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 1:1 serial dilutions of UPF3B-WT were then added to the nucleotide solutions and incubated on ice for 30 mins before supplementing with 7.5% (final) glycerol and running on a Novex 6% Tris-Glycine WedgeWell gel (Invitrogen #XP0006A) equilibrated in Novex Tris-Glycine Native Running Buffer at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: 500.000 MDA-MB-468 cells were transfected with 40 pmol siRNA upon seeding in 6 well-plates using Lipofectamine 3000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with 4 μg plasmid using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h culture ...
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bioRxiv - Neuroscience 2022Quote: ... The EBs were fed every other day for 6 days then transferred to the low adhesion 24-well plates in the induction media containing DMEM/F12 (11330032, Gibco), 1× N2 supplement (17502048 ...
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bioRxiv - Neuroscience 2022Quote: ... spheroids were transferred to an ultra-low attachment 6-well plate placed on an orbital shaker at 65 rpm and medium was changed to Neurobasal medium (Gibco ThermoFisher) supplemented with 2% B27 supplements (Gibco ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22 ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Physiology 2022Quote: ... Real-time PCR reactions were run on a QuantStudio 6 Flex machine using PowerUp SYBR Green master mix (Applied Biosystems) using gene specific primer sets (Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Staining of nuclei with 4ʹ,6-diamidino-2-phenylindole (DAPI) was performed with mounting solution (ProLong Diamond Antifade Mountant with DAPI (ThermoFisher)) or 50% glycerol in 1×PBS.
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bioRxiv - Genetics 2022Quote: ... fixed ovaries and eggs were incubated in a 300 nM DAPI stain solution (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) on a shaking platform (150-200 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... Myc-Arhgef18 and/or the septin complex (Flag-SEPTIN2, Flag-SEPTIN6, Flag-SEPTIN7, GFP-SEPTIN9) on 6-well tissue-culture plates using Lipofectamine 3000 (Invitrogen) and harvested 18–24 h later ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR analysis was performed on a 384-well plate with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies) using GeneAce SYBR qPCR Mix (Nippon Gene) ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR amplification was performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Inc.). Thermocycler conditions consisted of 2 min incubation at 50 °C followed by 10 min incubation at 95 °C and 40 cycles at 95 °C for 15 s and 55 °C for 1 min followed by denaturation at 95□ for 15 sec and melt curve analysis from 60□ (1 min ...
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bioRxiv - Genetics 2022Quote: Cells plated in 10 cm Petri dishes were lysed and scrapped in 6 mL ice-cold TRIzol™ Reagent (Invitrogen) added directly to the culture dish after removal of the growth medium ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice in FACS buffer and analyzed by flow cytometry on an Attune NxT 6 cytometer (ThermoFisher). Antibodies used are shown in Supplemental Methods ...
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bioRxiv - Immunology 2022Quote: ... bone marrow progenitors were collected from the femurs of adult C57BL/6 WT mice and 10 × 106 progenitor cells were cultured in RPMI media (Gibco) supplemented with 10% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... For glutamine starvation experiments cells were washed once with PBS and incubated for 6 hours in DMEM/F12 without glutamine and supplemented with 10% (v/v) dialyzed FBS (Gibco), 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: HeLa mito-mKeima cells were seeded at 200,000/well in a 6-well plate 12-16h prior to transient transfection with the indicated siRNAs (20 nM) with Lipofectamine RNAiMAX (ThermoFisher). The liposome/siRNA mixture was added to culture media for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Cell Biology 2022Quote: ... at a concentration of 350,000 cells/ml and were cultured in neuromesodermal progenitor cell induction medium (NMP media) containing Essential 6 media (Gibco, A1516401), 1.5 mM CHIR (Tocris biotech ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from ∼100 mg of flash-frozen hairy root tissue and qRT-PCR reactions were performed using a QuantStudioTM 6 (ThermoFisher) as previously described 19 ...
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bioRxiv - Molecular Biology 2020Quote: ... Trypsinized hippocampi were washed two times with the same HBSS buffer before being triturated 5-6 times with a fire-polished glass Pasteur pipette in BME (Invitrogen) supplemented with 10 percent FBS (VWR) ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...