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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... ISE6 cells were fixed with 4% paraformaldehyde followed by 1 x PBS washes and the coverslip was mounted on a slide using Antifade gold mounting reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) and observed under the Nikon W-1 Spinning Disk confocal microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cell Biology 2023Quote: ... which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher) operating at 4 °C and 100% humidity ...
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bioRxiv - Developmental Biology 2023Quote: ... The precipitated pellets containing the cumulus-oocyte complexes (COCs) were transferred to a 9 cm Petri dish and 6 ml of wash medium (TCM 199 - GIBCO, buffered with 2.5% HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Cell Biology 2023Quote: U2OS cells were transfected with 2.5 μg of plasmid per well (6 well plate) at approximately 80% confluence using Lipofectamine 2000 (ThermoFisher, 11668019) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... were maintained on gelatin-coated culture plates (6-well) in N2B27 medium consisting of 50 % DMEM/F12 (Catalog no. 21331020, Gibco) and 50 % Neurobasal medium (Catalog no ...
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bioRxiv - Bioengineering 2023Quote: ... in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI) or anti-phalloidin for F-actin (Invitrogen) at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Hepa1-6 (CRL-1830, ATCC), HEK293T (ACS-4500, ATCC), 3T3L1 (CL-173, ATCC) cells were cultured in DMEM medium (C11995500BT, Gibco) supplemented with fetal bovine serum (04-400-1A ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were seeded in a 6-well plate overnight before being transfected with 2.5 μM of siRNAs using Lipofectamine RNAiMAX transfection reagent (Invitrogen, USA) according to the manufacturers instructions ...
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bioRxiv - Neuroscience 2023Quote: ... live cells were imaged within 6-well plates at 20x magnification using an EVOS FL monochrome microscope (Invitrogen, catalogue #AMF4300) running Invitrogen image acquisition software ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme) on the QuantStudio 6 Flex (Life Technologies). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... biopsies were cut in small pieces containing all skin layers and plated on a 6-well plate coated with 0.1% gelatin with fibroblast extraction media (FEM; DMEM (Thermo Fisher), 20% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: One day after seeding 106 U2OS cells in 6-well plate, small interfering RNAs (siRNA, 20μM) were delivered using Lipofectamine® RNAi-MAX Reagent (Invitrogen) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The A1 mouse ES WT and Nsd1-KO lines 73 (background C57BL/6 × 129S4/ SvJae F1) were obtained from David Allis lab and maintained on gelatin-coated plates in Knockout DMEM (Gibco) supplemented with 15% ES-cell-qualified FBS (Gemini) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL of this cell suspension was plated into 3 wells on a bottom layer of 0.5% select agar in RPMI+10% FBS in 6-well non-tissue culture treated dishes (Thermo Scientific). For soft agar inhibitor experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Two hundred and fifty thousand NIH3T3 cells plated in a 6-well plate were transfected in the presence of Lipofectamine 2000 (Invitrogen) (per well 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium ...
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... Hepatic endoderm specification was initiated on differentiation days 6 ─ 10 using RMPI supplemented with 2% B27 and insulin (ThermoFisher, #17504044), 10ng/ml FGF-2 ...
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Developmental Biology 2024Quote: NIH/3T3 or HEK293T cells were plated at 1.5×105 cells per well in 6-well dishes and transfected 24 h after plating using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced serum medium (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The dissociated cells were added at 200,000 cells/well to 6-well plates containing astrocyte-coated coverslips in filter-sterilized NBA plus [Neurobasal-A medium (Gibco) supplemented with 1X Glutamax (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... After 7 days of differentiation the BMDMs were seeded at a density of 0.8×106 cells/well in 6-well plates in MGM 10/10 media containing Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco®) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Cancer Biology 2024Quote: ... 5E6 (5 × 10^6) GSU-Redfluc cells were suspended in 100 uL DPBS (Dulbecco’s Phosphate Buffered Saline; Gibco Inc., USA) and xenografted subcutaneously into NSG mice ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cells from above were transferred into a new low adherent 6-well plate (Fisher Scientific. Catalog No.7200601) with ∼ 300k cells per well ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out in 96-well plates using gene specific primers and a VWR qPCR Master Mix (Avantor) for 40 cycles on a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... with real time quantitative gene expression determined using using Taqman probles (Table 2) and analysed by the Quant Studio 6 Flex Real-Time PCR System (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Biophysics 2024Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) in phosphate buffered saline (PBS) and cells were mounted with ProLong Gold Antifade reagent (Invitrogen).
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bioRxiv - Biophysics 2024Quote: ... together with 0.5 μg psPAX2 packaging vector and 0.5 μg pMD2.G envelope vector DNA were co-transfected to 90% confluence HEK293T cells in a 6 cm petri dish using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells were infected with lentivirus for 4-6 hours before selection in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... the sections were rinsed in PBS and the nuclei were counterstained and mounted with Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI; #S36939, Invitrogen). The slides were imaged at a 4X magnification using EVOS M7000 (ThermoFisher Scientific™ ...
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bioRxiv - Microbiology 2024Quote: ... 1.0 µg of total RNA was resuspended in 2x formamide loading buffer (Thermo) and run on a precast 6% PAGE gel (Invitrogen) with the Riboruler RNA ladder (Thermo) ...
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bioRxiv - Microbiology 2024Quote: ... The human fibrosarcoma cell line HL116 stably expressing the firefly luciferase reporter gene under the control of the immediate early IFN-I inducible 6-16 promoter (kindly provided by Dr. S. Pelligrini, Institut Pasteur, France49) was maintained under HAT selection (Gibco; 21060017 ...
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bioRxiv - Neuroscience 2024Quote: 3 to 6 dpf-zebrafish larvae were placed in a 48-well plate (one larva per well, Fisher Scientific, USA) filled with embryo medium ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were derived from twenty-month old mice (C57BL/6 strain) and stimulated for 48 hours with CD3/CD28 Dynabeads (11452D, Gibco). The cells were then transduced with p-SIREN-sh lentivirus particles to target sestrins or with control p-SIREN scramble vectors ...
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bioRxiv - Immunology 2024Quote: ... Bacterial suspensions were further centrifuged at 3,000 rpm during 30 minutes at 6 °C and resuspended in the same volume of RPMI 1640 broth (Gibco, USA) for intranasal piglet infection ...
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bioRxiv - Microbiology 2024Quote: The protein concentration of gradient fractions 6 and 17 was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). Ten micrograms of the purified fractions 6 (exosome fraction ...
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bioRxiv - Neuroscience 2024Quote: ... 4 EBs/well were transferred to an Ultra-low attachment 6-well plate (cat#: 07-200-601; Thermo Fisher Scientific) and maintained in Maturation Medium (STEMdiff™ Cerebral Organoid Basal Medium 2 + Supplement E + 100 µg/mL Primocin ...
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bioRxiv - Biochemistry 2024Quote: ... For 10 ml of expression tests 107 cells are harvested by centrifugation (1400 rpm for 6 min) and resuspended in RPMI 1640 medium (Invitrogen) containing 0.1 % pluronic at a concentration of 20 x 106 cells /ml ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 cells per well were seeded in a 6-well plate and were transfected with Lipofectamine 3000 according to the manufacturer’s protocol (Invitrogen L3000015). For the transfections ...
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bioRxiv - Cell Biology 2024Quote: ... cells were reverse transfected with 1ug plasmids in a 6-well plate using JetOptimus (Polyplus#101000025) or Lipofectamine2000 (Thermofisher#11668019) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded in coverslips treated with poly-L-lysine (10 μg/mL) and grown for 6 days in Neurobasal medium (Gibco) supplemented with 0.05% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... the synthesized cDNA was subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Synthetic Biology 2024Quote: ... HEK 293T cells were cultured in 6-well plate and transfected at approximately 80% confluency in opti-MEM reduced serum medium (Gibco). Per each well ...