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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Differentiation to ECs were initiated by treating the hiPSCs with 6 μM CHIR99021 in RPMI-B27 without insulin media (Life Technologies) for 2 days ...
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bioRxiv - Bioengineering 2019Quote: B-cells were isolated from two C57BL/6 mice via MACS depletion (CD43 microbeads, 130-049-801 MACS) and labelled with Cell Trace Violet (ThermoFisher, C34557) or Cell Trace Far Red (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293F cells were cultured at 37 °C with 6% CO2 and 70% humidity in Free Style 293 medium (Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS).
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR (qPCR) was performed on a QuantStudio 6 Flex (Thermo Fischer Scientific) with Fast SYBR Green Master Mix (Life Technologies). Thermocycling parameters were defined as 95°C for 20 sec followed by 40 cycles of 95°C for 1 sec and 60°C for 20 sec ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Neuroscience 2019Quote: ... To preserve a more intact hair cell intracellular milieu we also performed perforated-patch recordings using an internal solution with gramicidin (40-50 μg/ml; Fig. 5A and 6) plus a fluorescent dye (Alexa Fluor 488 Hydrazide; ThermoFisher Scientific). Hair cells containing dye in their cytoplasm were excluded from analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.3 million cells were platted in a 6 well dish and cell proliferation was measured using trypan blue (Invitrogen, Carlsbad, CA) staining.
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bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... BEAS-2B cells (2.5 × 105/well in 6-well plate) were transfected with CRISPR-Cas9 plasmids by Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 16 EBs/well were plated in a 6-well tissue culture polystyrene plated coated with Matrigel™ in hematopoietic myeloid medium (HIM) consisting of 2mM GlutaMax (ThermoFisher), 55 μM beta-mercaptoethanol ...
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bioRxiv - Genomics 2021Quote: ... Cells (100000 cells/well in 6-well plates) were reverse-transfected in antibiotic-free medium by 25 nM miRNA mimic (ThermoFisher, 4464066) or control mimics (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: RBDwt was conjugated to CuMVTT using the cross-linker succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, MA, USA) at 7.5 molar excess to CuMVTT for 30 min at 25 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make) with the QuantStudio 6 Flex Real-Time PCR system (Life Technologies). The mRNA levels of ß-actin were used as an internal control to normalize the mRNA levels of Hsp70s ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: Each gel electrophoresis reaction used 6 µL of unbound B56 protein-antibody complex and 2 µL of 4x SDS-PAGE sample buffer (Thermo Fisher). After heating the samples to 95 C for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR reactions were performed in triplicate on QuantStudio 6 Flex Real-Time PCR Systems (Cat#4485694, Applied Biosystems, Waltham, USA) using the TaqMan Fast Advanced Master Mix (Cat#4444963 ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Eluates were heated to 65°C for at least 6 h to reverse the formaldehyde cross-linking and then treated with proteinase K (Life Technologies). DNA fragments were isolated using a DNA purification kit (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... accessed 6/18/2019) and the pepsin amino acid sequence using Proteome Discoverer software (version 1.3, SEQUEST algorithm, Thermo Fisher Scientific) or Byonic software (Protein Metrics ...
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bioRxiv - Neuroscience 2021Quote: ... and placed in the QuantStudio 6 Pro Real-Time PCR system (all reagents for RT-qPCR were purchased from ThermoFisher Scientific). The PCR cycle protocol was ...
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: Neuro-2a cells seeded in 6-well plates (6×105/well) were transfected with 100 nM circTulp4-specific or negative-control siRNA by using Lipofectamine® RNAiMAX (Invitrogen). At 24 h post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).
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bioRxiv - Developmental Biology 2021Quote: ... cells were plated in 24-wells 24 hours prior to lentivirus exposure and RNA lysates were collected 6 days later using TRIzol® (Invitrogen). For Western blotting ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were rinsed 6 times in PBS and left washing in PBS until mounted and coverslipped in ProLong Diamond Antifade Mountant (ThermoFisher #P36961) and then left to dry in the chemical cabinet overnight at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μg of the total proteins from each cell line were labelled with 6-plex isobaric tandem mass tag (TMT6) reagents (Thermo Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 450,000 mycoplasma-free cells were seeded into two wells of a 6-well plate in DMEM (11995-065; Thermo Fisher Scientific) containing 10% FBS (PS-FB3 ...
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bioRxiv - Cell Biology 2021Quote: ... 5×104 cells/cm2 were seeded on 6-well plates (Cell Coat, UK) and cultured in RPMI medium (Mediatech, Los Altos, CA, USA) containing GlutaMAX (Life Technologies), penicillin/streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Bone marrow was flushed from femurs and tibiae and bone marrow progenitor cells were cultured for 6 days on Petri dishes (Thermo Scientific) in Mφ medium [(DMEM high glucose ...
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bioRxiv - Cell Biology 2021Quote: Melanoma cells were seeded at a density of 6×105 cells/ml in 12-well plates and lysed on the next day with LDS buffer 1X (Life Technologies). Lysates were denatured at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... One microgram of siRNA was transfected in one well of a 6-well using Lipofectamine RNAiMAX transfection reagent according to the manufacturer protocol (Thermofisher Scientific). Cells were treated with siRNA for 24h.
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bioRxiv - Microbiology 2020Quote: ... HAP1 cells with a low passage number were seeded into 6 well microtiter plates at a density of 5 × 105 cells/well in IMDM medium (#21980032, ThermoFisher Scientific) containing 10 % FBS (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... were used to amplify viral RNA and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT-PCR system (Applied Biosystems). Relative quantities of viral and cellular RNA were calculated using the standard curve method (112) ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme) and a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). Transcript abundances were calculated using the delta-Ct method47 and normalized to the transcript levels of ACTIN2 (AT3G18780) ...
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bioRxiv - Plant Biology 2021Quote: ... approximately 0.2 grams of leaf petioles were homogenized using a HOMEX 6 homogenizer (Bioreba AG, Reinach, Switzerland) and TNA extracts were prepared using a MagMAX-96 viral RNA isolation kit (ThermoFisher Scientific) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted TNA samples were analyzed by reverse transcription quantitative PCR (RT-qPCR) using TaqMan probes on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously (Osman et al. ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with MVA or MVAΔE5R at MOI 10 for 6 h in the presence of MG132 (25 μg/ml) and lysed in RIPA lysis buffer (ThermoFisher, 89901) on ice for 30 min ...
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bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... total RNA from infected (MOI 0.01) Vero E6 cells (1.2 x 106 cells/well, 6-well format, triplicates) was extracted using TRIzol reagent (Thermo Fisher Scientific), and used in RT-PCR reactions to amplify a fragment of 1,174 bp around the RBD of the S gene ...
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bioRxiv - Neuroscience 2020Quote: ... Surviving cells were harvested on Day 2 and plated on either matrigel coated 24 well plates at a concentration of 100,000 cells/well for ICC studies or 1×106 cells/well of a 6 well plate for drug exposure studies in neural differentiation media consisting of Neurobasal media (Thermo Fisher), B27 (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).