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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... ESCs in one well of a 6-well plate were transfected with 10 μg of expression vector using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Human Drosha cDNA with a Flag-tag at the amino-terminus and a 6 x his tag at the carboxyl-terminus was cloned into pcDNA4/TO (Invitrogen) to construct the inducible WT Drosha expressing plasmid for immunoprecipitation assay and ubiquitination assay ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Cell Biology 2021Quote: 1×106 L1UP and Ctrl mESCs were seeded in 10 cm dish 16 hours prior to transfection with 6 μg of JJ-L1SM (WT and L1N21A) plasmid using Lipofectamine 3000 (Invitrogen). Media exchange was initiated 6 hours post transfection and hygromycin supplemented media was added 48 hours post transfection to select for stably transfected cells ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Neuroscience 2020Quote: ... Roughly 100,000 HPCs per well were seeded into a 6-well plate in custom made media containing DMEM/F12 (ThermoFisher, 11320033), 2X insulin transferrin selenite (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μg of pcDNA3.1+ empty vector were transfected into cells at least 6 hours post-siRNA transfection using lipofectamine LTX with Plus reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... ARPE-19 cells were transfected by reverse transfection in 24-well tissue culture plates as follows: A total of 6 pmols of siRNA was diluted in 100 μl of OptiMem (Gibco) per well ...
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bioRxiv - Neuroscience 2020Quote: ... the hippocampi of embryonic day 17 C57BL/6 mice (Koatech, South Korea) were dissected and digested with 0.125% Trypsin-EDTA solution (Gibco, USA) for 15 min in a 37 °C water bath ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (ATCC CDL-1586) were grown to 80% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 5% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was placed on slides and cover-slipped with mounting media containing DAPI (Fluoromount-G; 4’,6-diamidino-2-phenylindole; Invitrogen). All immunofluorescence runs contained tissue from more than one age (1 ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Synthetic Biology 2022Quote: ... The optical density of the cells was monitored by the UV-Vis spectrophotometer (Genesys 6, Thermo Fisher Scientific, Waltham, WA) (Figure S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Embryos were then washed in fresh E3 medium and kept in groups of 6 in 24-well polystyrene plates (Nunc) in 1 ml of E3 water ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Immunology 2022Quote: ... which had been placed in the wells of a 6-well plate filled with 1.1 ml phenol red-free RPMI medium (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... were mixed with 20 ng of genomic DNA and genotyped using a Quantstudio 6 Flex System according to the manufacturer’s instruction (Applied Biosystems).
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from rSARS-CoV-2/mCherry-Nluc-infected (MOI 0.01) Vero E6 cells (1.2 x 106 cells/well, 6-well format) using TRIzol reagent (Thermo Fisher Scientific) based on the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: 1×105 N2A or HEK293T (ATCC) cells were seeded in one well of a 6-well plate in 2mL of DMEM complete media (Gibco) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were counter stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in ProLongTM Gold antifade mounting media (Invitrogen). Images were captured using Nikon C2 confocal microscope.
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... The samples were then allowed to adsorb on the grid for 30s before blotting away the excess buffer using a blot force of 6 and blot time of 6s in a FEI Vitrobot Mark IV (ThermoFisher). The grids were plunge frozen in a liquid ethane slush and stored in liquid nitrogen until imaging ...
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bioRxiv - Immunology 2019Quote: ... Transient transfections of wild-type or mutated MCM10-expressing plasmids were performed by transfecting 2×106 cells with 6 μg of DNA using Lipofectamine 2000 according to manufacturers’ instructions (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) infected at multiplicity of infection (MOI) of 0.01 were extracted using TRIzol reagent (Thermo Fisher Scientific), according to the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with different GIPR constructs using Lipofectamine 2000 transfection reagent (Invitrogen). All the mutant constructs were modified by single-point mutation in the setting of the WT construct (HA-Flag-3GSA-GIPR(22-466)) ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... HUVEC seeded at 1.5 × 106 cells per well of a collagen-coated 6-well pate were transfected with plasmids using the Neon Transfection System (Invitrogen), harvested 48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on coverslips coated with poly-L-lysine in 6-well plates and cultured in Neurobasal medium (Gibco) supplemented with B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... The mice were tail vein-injected with 100 μL of 6 mg/mL Hoechst dye (Thermo Fisher Scientific, Waltham, MA) 4-6 h prior to termination of an experiment to visualize nuclei (57) ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... and the efficiency of rRNA depletion was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Assays were performed in analytical triplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Warrington, Cheshire, UK) and target gene expression levels were normalized to average expression of housekeeping gene GAPDH using ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were transfected with 0.25 μg/6 × 104 Hela cells of the myc-Rac1Q61L plasmid with turbofect (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Biochemistry 2021Quote: ... were seeded in 6-well plate with a cover glass inside and were transfected using lipofectamine 2000 (Thermo Fisher, #11668027) with mouse mEGFP M-Lip or mEGFP M-Lipxtal and mApple-Sec61b ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...