Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Larval fish were immobilized completely in 2% low-melting-temperature agar (Thermo Fisher), and the left eye freed ...
-
bioRxiv - Neuroscience 2021Quote: ... FISH was performed with the QuantiGene ViewRNA ISH Cell Assay (Thermo Fisher Scientific) protocol for adherent cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... Breakpoint-specific FISH were performed using the ViewRNA ISH Cell Assay (Invitrogen, QVC001) for with custom-made breakpoint-specific probes targeting the junctions of extrachromosomal DNAs ...
-
bioRxiv - Cell Biology 2020Quote: ... FISH was performed with the ViewRNA ISH Cell Assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... fish were embedded in 2-2,5% low-melting-point agarose (LMP, Fisher Scientific) in a recording chamber (Fluorodish ...
-
bioRxiv - Immunology 2021Quote: ... RNA-FISH was performed using the ViewRNATM ISH Tissue 1-Plex Assay (Affymetrix) with heat pretreatment for 10 min and protease digestion for 20 min ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Fish were rinsed 3X with PBS then incubated with DAPI (1:2000, Invitrogen) in PBS for 20 min at 22°C to label cell nuclei ...
-
bioRxiv - Neuroscience 2023Quote: ... and then blocked in a fish serum blocking buffer (Thermo Fisher, Cat #37527) for 1 hr at RT ...
-
bioRxiv - Systems Biology 2023Quote: ... IL6 FISH mRNA probe set (VA6-12712-VC) was purchased from Thermo Scientific. CF 488A succinimidyl ester (SCJ4600018 ...
-
bioRxiv - Cell Biology 2023Quote: ... FISH was performed using the ViewRNA ISH Cell Assay kit (ThermoFisher, cat# QVC0001) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... fish were laterally oriented in coverglass-bottom chamber slides (Thermo Fisher Scientific, #155383). PTU water supplemented with 0.1% (0.25× Tricaine ...
-
bioRxiv - Immunology 2024Quote: ... Fish EPC cells (Epithelioma papulosum cyprini cells) were maintained in medium 199 (Invitrogen) supplemented with 10% FBS ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... we prepared samples by adding 1 μL of dilute LIVE/DEAD sperm viability stain (Molecular Probes L-7011 ...
-
bioRxiv - Cell Biology 2022Quote: ... The sperm cells were resuspended in 540 μL of PBS and supplemented with DSSO (ThermoFisher Scientific) to a final concentration of 1 mM ...
-
bioRxiv - Developmental Biology 2022Quote: ... Sperm cells were collected by passing the solution through a 40-μm strainer (Fisher Scientific, #22363547) followed by three washes with phosphate-buffered saline (PBS) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fish were then mounted in a drop of 0.8% low melting point agarose (Invitrogen) over a glass-bottomed plate and imaged using an inverted Zeiss LSM 880 confocal microscope ...
-
bioRxiv - Genomics 2020Quote: ... smRNA-FISH was performed using the viewRNA Cell Plus Assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Fish were then immediately euthanized by submersion in ice and stored in RNAlater (Invitrogen). One offspring from family C1 stayed at the bottom the entire assay ...
-
bioRxiv - Cell Biology 2021Quote: ... blocked for 1 hr in PBS containing 1% BSA/0.2% Fish Gelatin (Fisher Scientific), and probed for membrane TRPV4 using 1:200 rabbit anti-TRPV4 (Alomone Labs ...
-
bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2019Quote: ... Fish were then mounted in a drop of 1.6% low melting point agarose (Invitrogen) over a glass-bottomed plate.
-
bioRxiv - Developmental Biology 2020Quote: Scales were manually detached from anesthetized fish and pre-treated with 100mM DTT (Invitrogen) before O/N fixation in 4 % PFA ...
-
bioRxiv - Genomics 2021Quote: Coverslips after either K562 or HCT116 FISH were mounted on glass slides (Fisher Scientific) with DAPI-containing antifade mounting media (0.3 μg/ml DAPI (SigmaAldrich)/10% w/v Mowiol 4-88(EMD Millipore)/1% w/v DABCO (Sigma-Aldrich)/25% glycerol/0.1 M Tris ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA FISH was performed using the ViewRNA ISH Cell Assay kit (ThermoFisher Cat# QVC0001) with probes for Involucrin (ThermoFisher Cat# VB1-3030396-VC ...
-
bioRxiv - Genetics 2019Quote: ... The fixed fish were embedded in optimal cutting temperature compound (Neg50TM, Thermo Fisher Scientific) and quick-frozen in −150 °C nitrogen freezer ...
-
bioRxiv - Genetics 2020Quote: ... Fish were anesthetized with 1X tricaine and mounted in 1.5% agarose (Fisher Scientific, USA) prepared with 1X tricaine solution (0.18 mg/L ...
-
bioRxiv - Developmental Biology 2021Quote: Fish were incubated with 0.1 mg/ml of 5-ethynl-2-deoxyuridine (Thermo Fisher) in embryo media for 2 hours prior to harvesting at 98 hpf ...
-
bioRxiv - Genetics 2022Quote: For flow RNA fluorescence in-situ hybridization (Flow-FISH) the PrimeFlow RNA assay (Thermofisher) was used according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2022Quote: ... FISH was performed using QuantiGene ViewRNA ISH Cell Assay (Affymetrix, Santa Clara, CA, USA). For multiplexing ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA-FISH was performed using the QuantiGene ViewRNA ISH Cell Assay kit (Invitrogen, QVC0001), according to manufacturer’s instructions ...
-
Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Live hSpermatozoa in sperm wash medium were incubated with 1 mM MitoTracker™ (Molecular Probes; M7512, USA) for 45 min at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: Changes in Ca2+ concentration in sperm cytoplasm were detected using 5 μM calcium indicator Fluo4-AM (Invitrogen) containing 0.1% Pluronic-acid F-127 and incubated at 37 °C under 6% CO2 in humidified air for 30 min in the dark 57 ...
-
bioRxiv - Physiology 2020Quote: ... Cell survival was assessed by adapting a LIVE/DEAD sperm viability kit (Molecular Probes, Eugene, OR, USA) to discriminate between undamaged and freezing-damaged cells (Yi and Lee ...
-
bioRxiv - Immunology 2022Quote: A FluoroNunc Maxisorp 96-well plate was coated with 100 μg/mL salmon sperm dsDNA (AM9680, Invitrogen) in PBS and incubated overnight at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Spermatozoa membrane viability was assessed using the LIVE/DEAD Sperm Viability Kit (Invitrogen/Thermo Fisher Scientific Inc.). A flow cytometry with the S3eTM Cell Sorter (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... Spermatozoa membrane viability was assessed using the LIVE/DEAD Sperm Viability Kit (Invitrogen/Thermo Fisher Scientific Inc.). A flow cytometry with the S3eTM Cell Sorter (Bio-Rad ...
-
bioRxiv - Developmental Biology 2021Quote: ... Anesthetized fish were injected intraperitoneally with 5 µl of 1 mg/mL EdU (Thermo Fisher) in sterile PBS ...
-
bioRxiv - Molecular Biology 2021Quote: RNA FISH was performed using a ViewRNA cell Plus Assay Kit (Invitrogen, #88-1900-99) following the manufacturer instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... fish were mounted dorsal-up in 2% (w/v) low melting agarose in PBS (Invitrogen) and imaged at ~0.8/0.8/2 µm voxel size (x/y/z ...
-
bioRxiv - Genomics 2021Quote: ... fish were injected with 100 µL of PBS (Phosphate Buffered Saline, pH 7.4 Life Technologies) in their abdominal cavity in order to sample their fluids to detect infection by S ...
-
bioRxiv - Genomics 2021Quote: ... RNA was extracted from 3 week old SAT fish using TRIzol™ Reagent (Fisher Scientific) according to manufacturer’s protocol and all residual DNA was removed using DNA-free™ DNA Removal Kit (Life Technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10ul of SlowFade® Gold anti-fade reagent (Invitrogen RNA FISH Kit, Cat. No. F32956) and 1ug/ml DAPI were added to the slide which was then covered with a coverslip and sealed with nail polish.
-
bioRxiv - Molecular Biology 2020Quote: ... 10ul of SlowFade® Gold anti-fade reagent (Invitrogen RNA FISH Kit, Cat. No. F32956) and 1ug/ml DAPI were added to the slide which was then covered with a coverslip and sealed with nail polish.
-
bioRxiv - Developmental Biology 2020Quote: Total RNA from 6-week-old fish was extracted with Trizol reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... FISH-labelled samples were air-dried and mounted in ProLong Gold antifade reagent (Life Technologies) containing 4’,6-diamidino-2-phenylindole (DAPI).
-
bioRxiv - Developmental Biology 2023Quote: ... Fish larvae were cleared in 80% glycerol and mounted in custom-made coverslips (Fisher Scientific). Meckel’s length ...
-
bioRxiv - Neuroscience 2023Quote: ... Juvenile fish processing required the addition of a decalcification step in Cal-Ex (Fisher Scientific) following fixation for 1 hour and 15 minutes rocking at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Then fish were mounted in mounting solution (ProLong Gold antifade reagent with DAPI, Invitrogen, USA).
-
bioRxiv - Bioengineering 2021Quote: ... Sperm cells were stained with 1 µL/mL LIVE/DEAD™ Fixable Aqua Dead Cell Stain (L34957, Invitrogen) and left at room temperature (with container wrapped in aluminum foil ...