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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... clipped grids (modified Autogrids with FIB notch) with plunge-frozen mouse sperm were transferred to an Aquilos dual-beam instrument with cryo-sample stage (Thermo Fisher Scientific). Two layers of platinum were added to the sample surface to enhance sample protection and conductivity (sputter-coater ...
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bioRxiv - Physiology 2022Quote: ... The colony was then removed and the SW containing live sperm (henceforth referred to as “sperm water”) was passed through a 100 μm cell strainer into a 50 mL conical tube (Thermo Fisher Scientific). This process was repeated for each spawning male ...
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bioRxiv - Developmental Biology 2021Quote: Sperm were isolated from 2-4 wild-type and mutant male fish and kept in 200 μl Hank’s saline containing 0.5 μM MitoTracker Deep Red FM (Molecular Probes) on ice ...
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bioRxiv - Developmental Biology 2021Quote: Sperm were isolated from 2-4 wild-type and mutant male fish and kept in 150 μl Hank’s saline containing 0.5 μM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... coated 6-well plates or glass coverslips (for smRNA FISH purposes) in Essential 8 Flex medium (Thermo Fisher Scientific) with E8 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: Spermatozoa were squeezed from 2–4 wild-type and mutant male fish and kept in 150 µL Hank’s saline containing 0.5 µM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Each eye (between the lens and the retina) of the fish was injected either with 1% DiI (Invitrogen, #D275) or 1% DiO (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and RNA FISH flow was performed for MYC and FGFR2 using the PrimeFlow™ RNA Assay Kit (Thermo Fisher) following the manufacturer’s protocol and corresponding probe sets (MYC ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the dissected and isolated forebrain of each adult fish using homogenization with TriZol (Ambion) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... embryos (20/treatment) were rinsed with fresh fish water and added to 500 μl of Trizol (Thermo Fisher Scientific) for RNA preparation.
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from ontogenetic and regenerating scales (n=3 fish per group, RU) by using Trizol (Invitrogen) for RNA-sequencing (RNA-seq ...
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bioRxiv - Cell Biology 2020Quote: ... were processed for RNA FISH analysis according to the manufacturer’s protocol (ViewRNA ISH Cell Assay Kit) (Thermo Fisher, #QVC0001). mRNA probes were hybridized at 40 °C for 3 hr ...
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bioRxiv - Neuroscience 2023Quote: Slices were incubated in blocking buffer solution (1% bovine serum albumin, 0.2 % fish skin gelatin, 0.1 % Triton, or 1 % Triton in 1x PBS; BSA from Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: TR14 cells for interphase FISH were grown in 8-chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™) to 80 % confluence ...
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bioRxiv - Cancer Biology 2023Quote: TR14 cells for interphase FISH were grown in 8-chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™) to 80 % confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... The slides were then washed twice in 1x PBS and used for Affymetrix FISH experiments as described in the manual of the QuantiGene ViewRNA ISH Cell Assay (Affymetrix), protocol for glass slide format ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed twice with PBS and then incubated with the desired combination of RNA FISH probes (Fig. S4A; Table 3; Affymetrix) in Probe Set Diluent at 40oC for at least three hours ...
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bioRxiv - Genetics 2019Quote: Total RNA from adult fish tissues and approximately 50 embryos/larvae per developmental stage was isolated using TRIzol® (Invitrogen) and cleaned up with the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: For t-FISH asynchronous HCT116 populations were incubated for 1 hr in 0.25 µg/mL KaryoMAX colcemid (Gibco 15212-012). Cells were collected and washed in 75 mM prewarmed KCl ...
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bioRxiv - Developmental Biology 2021Quote: ... smRNA FISH was carried out in accordance with the manufacturer’s protocol for the ViewRNA TM ISH Cell Assay (QVC001; ThermoFisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: GAS5 RNA probes (sense/antisense) were synthesized and labeled using the FISH Tag RNA Multicolor kit (Life Technologies, Gaithersburg, MD). Skin tissue cryosections were digested with 20 µg/ml of proteinase K at 37 °C for 1 hour ...
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bioRxiv - Pathology 2021Quote: ... posterior intestine and some skin mucus (collected with a cotton swab in the lesion area in the case of symptomatic fish) were individually and aseptically sampled and preserved in 500 μl RNAlater (Ambion) at −80°C ...
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bioRxiv - Immunology 2021Quote: To determine the presence of GPR55 in B cells we performed fluorescent in situ hybridization (FISH) by using a murine Gpr55-probe (VB6-3216575, Affymetrix). The splenic sections were cut and collected in RNAse free slides (slides cleaned with RNAse ZAP) ...
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bioRxiv - Immunology 2020Quote: The proliferation of B cells in skin and head kidney were assessed in control and survivor fish by injection wih EdU (Invitrogen) as previously reported (7) ...
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bioRxiv - Molecular Biology 2022Quote: ... The slides were then washed twice in 1x PBS and used for Affymetrix FISH experiments as described in the manual of the QuantiGene ViewRNA ISH Cell Assay (Affymetrix), protocol for glass slide format ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Biophysics 2023Quote: ... we transferred the fish to a small tank filled with 7L of tank water with buffered MS-222 (Acros Organics) solution (0.02% ...
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bioRxiv - Neuroscience 2023Quote: ... the total RNA of 4 eyes from two fish was isolated using TRIzol (or TRI-reagent; AM9738, Thermo Fisher scientific) (Rio et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Stock probes were diluted 1:1000 in RNA FISH hybridization buffer preheated to 42°C (15% deionized formamide, 10% dextran sulfate, 2× SSC from Ambion, 0.02% BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... in facility water (2-month-old fish) or an E3 solution supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU; Acros Organics) (larvae) ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... mRNA detection (FISH) in sorted T cells was carried out using the ViewRNA Cell Plus Assay kit (Cat. No. 88-19000, Invitrogen). The slides were imaged using a Zeiss LSM800 confocal microscope and analyzed with ZEN software (Carl Zeiss ...
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Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiationbioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from the LCM samples and sperm cell enriched samples using the ARCTURUS® PicoPure® RNA Isolation Kit (Applied Biosystems®) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from NS and HS sperm cells or pollen grains using the Invitrogen™ TRIzol™ Plus RNA Purification Kit (Thermo Fisher Scientific). Approximately 0.2 g pollen were each collected in a 2 mL microcentrifuge tube with one metal balls inside ...
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bioRxiv - Genomics 2020Quote: ... where 2–3 mm of the terminal ends of 3–4 filaments from the left side each fish were collected and placed in RNAlater (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... The next day the water supply was shut off and the fish were anesthetized by carefully pouring a diluted benzocaine solution (Fisher Scientific, Acros 150785000 ...
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bioRxiv - Pathology 2022Quote: ... Amplified PCR products (766 bp) from each fish were isolated using NucleoSpin™ Gel PCR Clean-up Kit (Fisher Scientific, Sweden) according to the manufacturer’s protocol and then submitted for sequencing service (U2Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... from six randomly chosen fish (mentioned earlier) were collected and stored in sterile tubes supplemented with 200 μL Trizol (Invitrogen, USA) at −20 °C until examinations.
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bioRxiv - Microbiology 2020Quote: ... 10 triploid and diploid fish were euthanized and gill and heart tissue were sampled and placed in 1 mL RNAlater (Thermo Fisher). All fish were kept at 12°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... fish were anesthetized and mounted laterally in 1.5% low melt agarose within glass bottom microscopy dishes (Nunc Glass bottom dishes 27mm). ‘Embryo pokers’ were used to orient the embryos onto their sides ...
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bioRxiv - Biophysics 2022Quote: ... Cells were washed with 2x saline-sodium citrate (SSC) two times followed by a final wash with FISH wash solution (10% formamide (Ambion, #AM9342) in 2x SSC) ...
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bioRxiv - Genomics 2022Quote: ... Slides were then placed in a hybridization chamber, and 75μl of preheated FISH solution (0.9M NaCl, 20mM Tris pH 7 (both Thermo Fisher Scientific, USA), 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Double FISH samples were incubated in Sodium Azide solution (100 mM sodium azide [Fisher Scientific] in PBS + 0.3% TritonX-100 (PBSTx)) to inactive peroxidase activity for 45 minutes before performing secondary signal development.
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bioRxiv - Immunology 2023Quote: ... the fish were first completely euthanized and then immobilized in a 1-well glass-bottom chamber (Nunc Lab-Tek II 155360) with low gelling temperature agarose (Sigma Aldrich 19045) ...
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bioRxiv - Immunology 2022Quote: Kidney marrow cells of adult Tg(lysC:dsRed)nz50Tg/ Tg(BACmmp9:Citrine-CAAX)vi003 fish (aged three months) were FACS sorted into Mmp9+ LysC+ or Mmp9- LysC+ fractions and spun in a cytocentrifuge (Fisher Scientific) onto slides according to the manufacturer’s instructions and stained using Pappenheim solution (Merck).
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bioRxiv - Neuroscience 2024Quote: ... both retinas from either three (6-week-old) or two (12- or 18-week-old) fish were pooled and collected in Leibovitz 15 (L15, ThermoFisher Scientific) medium complemented with 2% Penicilin-Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed 3 times in cold PBS and blocked in FISH blocking buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: ... The head was dissected from fish body and preserved in RNAlater™ Stabilization Solution (Cat: AM7021, Invitrogen, Thermo Fisher Scientific Inc.) at -80°C freezer ...
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bioRxiv - Genomics 2024Quote: ... The head was dissected from fish body and preserved in RNAlater™ Stabilization Solution (Cat: AM7021, Invitrogen, Thermo Fisher Scientific Inc.) at -80°C freezer ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the ImageJ software (26) ...