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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the nuclear extract was pre-incubated for 20 min at 30°C with the nonspecific Salmon Sperm DNA (Invitrogen): 1 µg of DNA per 1.5 µl of the nuclear extract (approximately 5 µg ...
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bioRxiv - Genetics 2019Quote: ... A 7 μl aliquot of the pooled library was aliquoted and mixed with 0.8 μl of a 10 μg/μl UltraPure Salmon Sperm DNA Solution (Invitrogen) in a PCR-tube ...
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bioRxiv - Biochemistry 2023Quote: ... AAV libraries were first reacted with 1 nmol biotinylated ssDNA oligos (sequences are given in Supplemental Table 3) in PBS supplemented with 0.05 % v/v salmon sperm DNA (Invitrogen), 1 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... pre-blocked with salmon sperm (Thermo Fisher, # 15632011) were used to precipitate antibody complexes from diluted samples ...
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bioRxiv - Cancer Biology 2022Quote: ... The FISH probes were from ThermoFisher: mouse Trp53 Type 4 488 probe (VA4-3082421-VC ...
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bioRxiv - Genetics 2023Quote: ... Strand-specific RNA-FISH (ssRNA FISH) probes were produced using the MAXIscript T3/T7 kit (Ambion, #AM1326). ssRNA FISH probes were labelled using FITC-UTP and purified through Mini Quick Spin RNA columns (Roche ...
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bioRxiv - Genetics 2021Quote: ... followed by the addition of 40 μl of denatured carrier DNA solution (salmon sperm ssDNA, 10 mg ml-1; Invitrogen). Aliquots of 50 μl of these competent cells were used immediately for transformation (for maximum efficiency ...
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bioRxiv - Genomics 2020Quote: ... DNA extracted from whole blood and sperm was quality controlled using a Qubit 3.0 Fluorometer (Life Technologies, Cat No. Q33216). DNA was stored in −80°C prior to bisulphite conversion.
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bioRxiv - Synthetic Biology 2022Quote: ... The ADP-ribosylation reactions contained 0.2 μM purified PARP-1 or PARP-3 and 100 ng/μL of sheared salmon sperm DNA (Invitrogen, AM9680). Purified proteins were mixed with the indicated amounts of NAD+ in ADP-ribosylation Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Each plate included four DNA standards diluted from known concentrations of synthesized gene fragments (IDT gBlocks) with the addition of nonspecific salmon sperm DNA (ThermoFisher). All plates included control reactions with water in place of the cDNA template (no-template controls ...
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bioRxiv - Developmental Biology 2019Quote: ... and hybridization was added more yeast RNA (100 μg/mL) and Salmon Sperm DNA (100 μg/mL) (Thermo Fisher Scientific). For post-hybridization ...
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bioRxiv - Microbiology 2021Quote: ... which had been washed three times in lysis buffer and blocked with 100 mg/mL sheared salmon sperm DNA (Ambion), were added to chromatin-antibody mixture and incubated at 4°C for 4 hours ...
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bioRxiv - Pathology 2021Quote: ... The DNA was then spiked at a final concentration of 1.33 ng μl-1 in salmon sperm gDNA (SSD, Thermo Fisher) at 10 ng μl-1 in artificial seawater (ASW ...
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bioRxiv - Neuroscience 2022Quote: ... plates were washed twice with TBST and blocked with 5% BSA + 0.1 mg/ml salmon sperm DNA (ThermoFisher, cat# 15632011), 100ul/well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The total amount of transfected DNA was adjusted and kept constant at 1 µg per mL of cell culture to be transfected using salmon sperm DNA (Invitrogen) as ‘carrier’ DNA ...
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bioRxiv - Physiology 2021Quote: ... Stained sperm samples were mounted with Prolong gold (Invitrogen) and cured for 24 hr ...
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bioRxiv - Immunology 2021Quote: ... and then subsequently coated with salmon sperm dsDNA (Invitrogen) or Smith ribonucleoprotein (SmRNP ...
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bioRxiv - Genomics 2021Quote: FISH probes were prepared using BACs (Invitrogen). BACs were extracted from bacterial culture using the NucleoBond® Xtra Midi plasmid extraction kit (Macherey-Nagel ...
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bioRxiv - Zoology 2022Quote: ... the live and dead sperm cells were stained green and red fluorescence respectively using LIVE/DEAD™ Sperm Viability Kit (Thermo Fisher Scientific). Ten microlitre SYBR-14 working solution diluted 50-fold with each solution was added into 10 μL out of the 40 μL sperm suspension and incubated for 10 min ...
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bioRxiv - Genetics 2019Quote: ... Chromatin-antibody complexes were recovered using pre-blocked (1mg/ml BSA and 1ug/ml salmon sperm DNA) Dynabeads Protein A (Life technologies). Purified DNA was analysed by quantitative PCR using Roche Lightcycler480 using SYBR green reagents (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... approximately 100 ng of labelled probes (enough for three 12 mm coverslips) were precipitated with 10 µg of sheared salmon sperm DNA (ThermoFisher Scientific) and 5 µg of human Cot-1 DNA (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA at a concentration of 1-2 mg/ml was mixed with 5 µl of Salmon Sperm DNA (10mg/ml) (Thermofisher Scientific) prior to electroporation ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Pathology 2022Quote: ... The probe was diluted in hybridization buffer (50% deionized formamide, 50% dextran sulphate, 50× Denhardt’s solution [Sigma-Aldrich, Germany], 20× SSC, 10 mg per ml salmon sperm DNA [Invitrogen, USA]), heated to 95°C for 10 minutes ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells stably expressing TfR-GFP were plated on fibronectin coated MatTek dishes and the next day incubated with 1 μM Alexa 647 labelled AP3 aptamer in blocking buffer (2 mM salmon sperm DNA, ThermoFisher #15632011), 2 mM yeast tRNA (ThermoFisher #AM7119 ...
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bioRxiv - Bioengineering 2022Quote: ... Serial dilutions of gene fragments were performed in sheared salmon sperm DNA at 5 µg mL−1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with 2× saline sodium citrate (SSC) buffer for 5⍰min and blocked with UltraPure Salmon Sperm DNA (Thermo Fisher) in QuikHyb Hybridization Solution (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2023Quote: ... or 300 μg (MS) of nuclear protein extracts alongside 20 μg (Western blot) or 40 μg (MS) of sheared salmon sperm DNA (Thermo Scientific), diluted in PBB buffer [(50 mM Tris,150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The leftover PFO with small amounts of aqueous phase on top was extracted once with a 100 µL solution of UltraPure Salmon Sperm DNA solution (Thermo Fisher), diluted 2500x in nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: We performed fluorescence in situ hybridization (FISH)/immuno-FISH using the QuantiGene®ViewRNA ISH Cell Assay Kit (Affymetrix) with few modifications from the manual ...
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bioRxiv - Neuroscience 2019Quote: ... fish were placed into fish water containing 1 mM BrdU and 0.333% of dimethyl sulfoxide (DMSO – Thermo Scientific, 20688). Treatments were performed in beakers ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then FISH-stained against SARS-CoV-2 ORF1a mRNA using ViewRNA mRNA FISH assay according to the manufacturer’s instructions (ThermoFisher) with some modifications ...
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bioRxiv - Genomics 2019Quote: ... To this mix 50 μg of denatured salmon sperm (Invitrogen), 500 ng of Cas9 plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μL salmon sperm (10 μg/ μL, Thermo Fisher Scientific), and 0.4 μL 25μM 5′ digoxigenin (DIG)-labeled CCCCGGCCCCGGCCCC locked nucleic acid probe (Exiqon ...
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bioRxiv - Zoology 2020Quote: We examined the PVL for sperm and germinal disc samples for embryonic cells by staining them with the fluorescent DNA marker Hoechst 33342 (Thermo Fisher Scientific), and inspecting them under a fluorescence microscope with a BP 340-380 excitation filter and LP 425 suppression filter (Birkhead et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed twice with ChIP RIPA buffer and blocked with 1 g/l BSA and 0.4 g/l sonicated salmon sperm DNA (Life Technologies no. 15632011) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of labelled probe was combined with 5 μg salmon sperm and 10 μg human C0t1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Genomics 2022Quote: ... 2.5% dextran sulfate, 0.2% BSA, 100 μg/mL salmon sperm DNA, 2X SSC, 0.1 U/μL SUPERase•In™ [Thermofisher] in DEPC H2O) was then pipetted into the chamber ...
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bioRxiv - Cell Biology 2019Quote: ... Fish were then incubated with Rhodamin-phalloidin (Invitrogen) diluted 1:100 in 2% PBSTx at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... fish were immersed in 500nM PLX3397 (Fisher Scientific) in system water ...
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bioRxiv - Microbiology 2021Quote: ... with (IF) or without (FISH) DAPI (Thermo Fisher). The following primary antibodies were used ...
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bioRxiv - Genomics 2021Quote: For Flow-FISH the PrimeFlow RNA assay (Thermofisher) was used according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... fish were macerated using 3% trypsin (Fisher Scientific) in 30% saturated sodium borate at 37°C for 2-4 hours ...
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bioRxiv - Physiology 2023Quote: ... Then fish were transferred in EDTA (500mM, Ambion) solution for another 72h and paraffin-embedded ...
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bioRxiv - Cell Biology 2019Quote: ... or 0.25 μl of oligo dT30 (stock 50 μM) was mixed with 2 μl of salmon-sperm DNA (10 mg/ml, Life Technologies, 15632-011) and 2 μl yeast transfer RNA (10 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Molecular Biology 2020Quote: ... Combined samples were resuspended in 110 µL of fresh hybridization mix (5 µL 10 mg/mL Salmon Sperm DNA (Thermo Fisher Scientific, 15632011), 5 µL 8 mg/mL Yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 μL of probe mix (stock 25 μM) was mixed with 2 μL of salmon-sperm DNA (10 mg/ml; Life Technologies, 15632-011) and 2 μL yeast transfer RNA (10mg/ml ...
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bioRxiv - Bioengineering 2019Quote: The Live/Dead Sperm Viability Kit was purchased from Molecular Probes L7011 (Thermo Fisher Scientific ...