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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... GH and actin-β in each individual fish were measured in duplicate using the “relative quantification” method (Applied Biosystems User Bulletin #2). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... IHC was performed concomitantly with the FISH detection with anti-rabbit Alexa Fluor 568 (1:500, A10042, Life Technologies, Carlsbad, CA USA) or anti-chicken Alexa Fluor 488 (1:500 ...
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bioRxiv - Physiology 2021Quote: Individual pituitaries from at least six maturing and six immature fish per time point were collected and stored in 300 μl TRIzol reagent (Invitrogen, Waltham, USA) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The jaw joint region of each fish and uninjured control (25 joints) was microdissected and incubated in protease solution [0.25% trypsin (Life Technologies, 15090–046), 1 mM EDTA ...
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bioRxiv - Zoology 2023Quote: ... Total RNA was extracted from the pooled intestine tissues of 6 fish in each pond above using TRIzol Regent (Thermo Fisher, USA). Primers were designed based on the coding sequences of these genes from large yellow croaker genome database (Ao et al. ...
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bioRxiv - Neuroscience 2023Quote: ... the total RNA of four eyes from two fish was isolated using TRIzol (or TRI-reagent; AM9738, Thermo Fisher, Waltham, MA, US)27 and quantified using a Nanodrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Tg[fabp10:mCherry] fish expressing mCherry exclusively in hepatocytes were generated using the MultiSite Gateway kit (Thermo Fisher Scientific, Waltham, MA, USA) to produce vectors with Tol2 transposon sites [57] ...
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bioRxiv - Genetics 2024Quote: Oocytes and eggs matured to the appropriate stage (and with the zona pellucida removed by Acidic Tyrode’s Solution (EmbryoMax, Cat# MR-004-D) for the FISH assay) were fixed in freshly prepared 2% paraformaldehyde (Thermo Scientific, Cat# 28908) in 1x PBS (Quality Biological ...
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bioRxiv - Neuroscience 2021Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Genetics 2020Quote: ... 250 ng of RNA per fish was used for reverse-transcription into cDNA using the Thermo Fisher SuperScript II kit (Thermo Fisher Scientific, USA). Requisite no reverse transcriptase (RT ...
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bioRxiv - Neuroscience 2022Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Neuroscience 2023Quote: ... Fish were euthanized 3 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Thermo Fisher, cat# S-11227). syt11b HCR in situ hybridization and HuC/D staining were performed on the same sections ...
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bioRxiv - Neuroscience 2023Quote: ... Fish were euthanized 3 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Thermo Fisher, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Neuroscience 2024Quote: ... the fish were paralyzed by immersing them for 75 seconds in 25 µL of a 1 mg/mL α-bungarotoxin solution (Thermo Fisher Scientific) in E3 medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked using 2.5% fish gelatin in a solution of 0.05% Tween20 in PBS followed by incubation with primary anti-Ki67 (ThermoFisher, rat, 14-5698-82), incubated over-night at 4°C ...
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bioRxiv - Immunology 2022Quote: ... DNA was eliminated using DNA-free™ DNA Removal Kit (Invitrogen). RNA quality was assessed by agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was removed via DNA-free DNA Removal Kit (ThermoFisher Scientific). RNA was normalized using concentrations from a Quant-iT PicoGreen dsDNA Assay (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... labeled with Alexa Fluor™ 488 were produced by in vitro reverse transcription from the T7 promoter using a FISH Tag RNA Kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Physiology 2020Quote: ... The second two gill arches from the left side of each fish and the liver were sampled and placed in RNAlater™ (Invitrogen™, Carlsbad, California, USA) and stored at 4°C overnight prior to storage at −80°C ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent, Thermo Fisher Scientific, Cat-No. NC0382999) at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was removed using DNA-free™ DNA Removal Kit (Invitrogen) and 1 μg of total RNA was reversed transcribed into cDNA using Promega Reverse Transcription System (A3500) ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was removed using DNA-free™ DNA Removal Kit (Invitrogen) and 500 ng-1µg of RNA was converted into cDNA using Promega Reverse Transcription System (A3500) ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was removed with DNA-free™ DNA removal kit (Thermo Scientific). All the RNA samples were of good quality with OD 280/260 ≥2.0 assessed with 2000 Nanodrop (NanoDrop Technologies ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was removed using DNA-free DNA removal Kit (ThermoFisher, AM1906). Then ...
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bioRxiv - Microbiology 2021Quote: ... Turbo DNA-free DNA removal kit (Invitrogen) was used to remove genomic DNA contamination ...
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bioRxiv - Microbiology 2022Quote: ... DNA-free DNA removal kit (ThermoFisher; AM1906) was used to remove potential genomic DNA contamination according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA ligation (T4 DNA ligase, Thermo Scientific), 5’ end phosphorylation (T4 polynucleotide kinase ...
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bioRxiv - Microbiology 2021Quote: ... followed by DNA Removal using DNA-free™ DNA Removal Kit (Thermo Fisher) and subsequent reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... and DNA was digested with DNA-free™ DNA Removal Kit (Applied Biosystems). RNA integrity was assessed on 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genetics 2022Quote: ... Contaminant DNA was removed with the DNA-free DNA Removal Kit (Invitrogen, AM1906). We used 1μg of RNA for reverse transcription (RT) ...
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bioRxiv - Genomics 2022Quote: ... DNA digestion was performed using the DNA-free DNA Removal Kit (ThermoFisher AM1906). Sequencing libraries of mRNA were prepared from 1 g total RNA using the NEBNext Ultra un-stranded preparation kit (E7775S,E7490S) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was cleaned from DNA with DNA-free™ DNA Removal Kit (Invitrogen). ∼1 µg of RNA was used to construct libraries with Truseq Stranded mRNA kit (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... and residual genomic DNA removed using DNA-free DNA removal Kit (Thermo Fisher Scientific). RNA was precipitated using 3 M sodium acetate and ethanol ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA contamination was removed using a DNA-free DNA removal kit (ThermoFisher; AM1906) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... DNA-free™ DNA Removal Kit (Ambion, USA) was used following the instructions of manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested with Turbo DNA-free (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was quantified using DNA PicoGreen (Life Technologies) and pooled in equal representation ...
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bioRxiv - Plant Biology 2023Quote: ... and DNA-free™ DNA Removal Kit (Invitrogen) was used to prepare DNA-free RNA according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Residual DNA in the sample was removed using DNA-free™ DNA removal kit (Invitrogen). Complimentary DNA (cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Contaminating DNA was removed from supernatants with the DNA-free DNA Removal Kit (Invitrogen, AM1906) and from infected cells and hamster tissues with the RNase-Free DNase set coupled with the RNeasy Midi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was removed by the DNase treatment kit (DNA-free™ DNA Removal Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... A DNA-free DNA removal kit was used to remove contaminated DNA (Thermo Fisher Scientific). RNA-seq poly-A libraries were generated with NEBNext UltraII directional RNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... Genomic DNA removal was performed using the DNA-free DNA Removal Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA contamination was removed using Invitrogen™ DNA-free™ DNA Removal Kit (ThermoFisher). 1 µg of RNA was used to prepare cDNA using the Sensifast cDNA synthesis kit (Bioline ...
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bioRxiv - Neuroscience 2022Quote: ... DNA contamination was removed from the RNA using DNA-free DNA removal Kit (Life Technologies). RNA purity and concentration were checked using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... and any residual genomic DNA was removed using a DNA-free DNA Removal Kit (Invitrogen). Sequencing libraries were prepared from purified RNA for two or three biological replicates using TruSeq RNA Library Prep kit v2 (Illumina) ...
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bioRxiv - Biochemistry 2024Quote: ... Residual DNA in the sample was removed using DNA-free™ DNA removal kit (Invitrogen). The RNA quality and quantity was assessed using the Experion™ RNA HighSens chips (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and residual genomic DNA was removed using DNA-free DNA removal Kit (Thermo Fisher Scientific) prior to ethanol precipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was removed using TURBO DNA-free Kit (Invitrogen). Quality of total RNA was determined by electrophoresis on the TapeStation system (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... followed by DNA-free™ DNA Removal Kit (Invitrogen). RNA integrity was confirmed using Agilent’s 2200 Tapestation ...