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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from epididymal sperm using Trizol reagent (Invitrogen) and ribosomal RNA was removed using the RiboMinus Transcrptome isolation kit (Invitogen ...
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bioRxiv - Physiology 2022Quote: ... 875 μL sperm water was combined with paraformaldehyde (Thermo Fisher Scientific) at a final concentration of 4% (v/v) ...
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bioRxiv - Cell Biology 2023Quote: Sperm lamellae were prepared using a Aquilos 2 SEM (ThermoFisher Scientific). First ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from pools of individuals (‘strain’ henceforth) derived from a single worm collected from a single experimentally infected fish using MagJET Genomic DNA kits (Thermo Scientific). DNA concentration was measured using Qubit High Sensitivity reagents ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to extract DNA from the muscle tissue of each fish and quantified it on a Qubit 3.0 fluoromether (Thermofisher Scientific, Inc.). Genomic libraries were then prepared at the Vincent J ...
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bioRxiv - Genetics 2023Quote: ... 6 non-overlapping probes of between 1.2 and 1.7kb covering an area of approximately 12kb were generated using the FISH Tag DNA kit with Alexa Fluor 555 or Alexa Fluor 647 dyes (Invitrogen Life Technologies). 100ng of each probe were added to the 30µL of FHB for hybridization.
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bioRxiv - Developmental Biology 2023Quote: ... to extract DNA from the fins of wild-caught adult fish and quantified it on a Qubit 3.0 fluorometer (Thermofisher Scientific, Inc.). All specimens used in this study are kept in the Museum of Vertebrate Zoology at the University of California ...
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bioRxiv - Microbiology 2024Quote: ... the PCR products were directly labeled with Alexa Fluor 555 dye and purified using the FISH Tag DNA Orange Kit (ThermoFisher Scientific). Labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... we generated double-stranded RNA-FISH (dsRNA FISH) probes through random priming of BAC templates (Xist, Atrx, Rnf12, Pgk1) using the Bioprime labelling kit (Invitrogen, #18094-011). Probes were labelled using Cy3-dUTP (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Fish were incubated in EdU (ThermoFisher, A10044 or C10340) at 500 μM for 24 hours ...
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bioRxiv - Biophysics 2022Quote: ... and salmon sperm DNA (850 ng/coverslip) were added to 100 µL Opti-MEM Reduced Serum Medium with GlutaMAX Supplement (Thermo Fisher Scientific; Cat# 51985026) and mixed well by vortexing ...
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bioRxiv - Genetics 2023Quote: ... 6 non-overlapping probes of between 1.2 and 1.7kb covering an area of approximately 12kb were generated using the FISH Tag DNA kit with Alexa Fluor 555 or Alexa Fluor 647 dyes (Invitrogen Life Technologies). 100ng of each probe were added to the 30µL of FHB for hybridization.
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bioRxiv - Cell Biology 2022Quote: Solubilized sperm proteins were quantified using EZQ Protein Quantitation Kit (Thermo Fisher) and 2.25ug alkylated (Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... were blocked with sonicated salmon sperm (ThermoFisher #AM9680, 200 mg/mL beads) and BSA (NEB #B9000S ...
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Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: RNAs were isolated from frozen sperm using mirVana miRNA Isolation Kit (Invitrogen). Bisulfite conversion of sncRNAs was conducted using the EZ RNA Methylation Kit (ZYMO Research) ...
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bioRxiv - Genomics 2020Quote: FISH probes were made from human BACs (ordered from Invitrogen, RP11-479I13 for HSPA1A locus and RP11-173P16 for HSPH1 locus) ...
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bioRxiv - Systems Biology 2020Quote: ... equilibrated in FISH Wash Buffer containing 2X SSC (Invitrogen 15557044) and 20% Formamide (Ambion AM9342 ...
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bioRxiv - Neuroscience 2020Quote: ... FISH was performed using the QuantiGene (QG) ViewRNA kit (Affymetrix) according to the manufacturer’s protocol using probes for Add2 ...
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bioRxiv - Neuroscience 2019Quote: ... fish were embedded in 1.5% low-melting-point agarose (Invitrogen) in a custom-designed ...
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bioRxiv - Neuroscience 2024Quote: ... Fish were incubated with 1.5 μM JC-1 (ThermoFisher, T3168) diluted in EM for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was prepared from adult mouse sperm using Trizol (Thermo Scientific 15596026) and Directzol (Zymo R2080) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sperm Culture Medium 199 (M199) was obtained from Thermo Scientific (Waltham, MA, USA). Bovine serum albumin was purchased from Solarbio (Beijing ...
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bioRxiv - Genetics 2019Quote: ... RNA was extracted from the fish tissues using Trizol reagent (Invitrogen) following protocols recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... FISH samples were mounted in ProLong® Gold Antifade Mountant (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... FISH was carried out following manufacturer’s instructions (QVC000; Thermo Fisher Scientific). Cells were mounted using DAKO mounting media or Prolong Gold with DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Fish were treated with 2.25 μM FM 1-43FX (ThermoFisher, F35355) for 1 minute in EM ...
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bioRxiv - Genetics 2020Quote: ... we: (i) use fluorescence in situ hybridization (FISH, Affymetrix PrimeFlow assay) to quantitatively label single cells according to their expression of an RNA of interest ...
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bioRxiv - Developmental Biology 2024Quote: ... whole fish minus internal organs were lysed in Trizol (Life technologies) using the Manufacturer protocol.
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bioRxiv - Developmental Biology 2021Quote: ... Sperm were spun onto an adhesive slide using a CytoSpin 4 (Thermo Fisher Scientific) at 1,000 rpm for 5 minutes followed by permeabilization with ice-cold methanol for 5 minutes and a wash with 0.1% Tween in 1x PBS (PBST) ...
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bioRxiv - Genetics 2020Quote: ... for sperm and adult extractions and a Qubit fluorometer dsDNA Broad Range kit (Invitrogen) for the larval extractions.
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bioRxiv - Genetics 2022Quote: Sperm localization was observed by mating N2 males stained with MitoTracker Red CMXRos (Invitrogen) to mutant hermaphrodites ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted from sperm samples using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the method provided by the manufacturer and quantified using ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Sperm was spun onto an adhesive slide with a CytoSpin 4 (Thermo Fisher Scientific) at 800 rpm for 3 minutes ...
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bioRxiv - Genetics 2023Quote: ... The remaining sperm samples were washed in 1X phosphate buffered saline (PBS, Life technologies), 10 µL were spread onto slides pre-coated with 0.1% poly-L-lysine (Epredia) ...
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bioRxiv - Developmental Biology 2024Quote: ... sperm were loaded with 2 mM of Fluo-4 AM (Invitrogen, Carlsbad, California, USA) diluted in 10% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... FISH was performed with ViewRNA ISH Cell Assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: FISH was performed with ViewRNA ISH Cell Assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: FISH assay was performed using target probes for NEAT1 (Invitrogen, VX-01) and the View RNA Cell Plus Assay (Invitrogen ...
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bioRxiv - Physiology 2019Quote: ... Fish were held singly in plastic containers (120 ml Fisherbrand; Fisher Scientific) in 60 ml of water under constant conditions (12:12 h light:dark cycle ...
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bioRxiv - Microbiology 2021Quote: ... FISH was performed 24 hours later using ViewRNA Cell Plus Assay (ThermoFisher) as manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... Fish were euthanized using an overdose of buffered MS-222 (Acros Organics) solution (0.04% ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: FISH was performed with the ViewRNA ISH Cell Assay kit (QVC0001, Invitrogen). Motoneurons were grown for 5-6 DIV on glass coverslips (0111500 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: Live embryos and fish embedded in 1% low-melting agarose (Life Technologies) or fixed samples were imaged using an LSM 710-Live Duo Confocal microscope (Zeiss) ...
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bioRxiv - Developmental Biology 2023Quote: RNA-FISH was performed using ViewRNA™ ISH Cell Assay Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse and human sperm were loaded with Cal-520-AM (5 μM, Molecular Probes, USA) in the presence of Pluronic F-127 (0.02% v/v ...
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bioRxiv - Zoology 2019Quote: ... RNA was extracted from sperm that was collected following the TRIzol procedure (Invitrogen, Life Technologies). The concentration was measured using Qbit and adjusted to 1ng/ul before injection into zygote.
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bioRxiv - Zoology 2019Quote: ... RNA was extracted from sperm that was collected following the TRIzol procedure (Invitrogen, Life Technologies). The concentration was measured using Qbit and adjusted to 1ng/ul before injection into zygote.
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bioRxiv - Zoology 2019Quote: ... the viability of cells was checked by viability stain (Live/Dead Sperm viability kit (ThermoFisher L7011 ...
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bioRxiv - Genetics 2023Quote: ... the sperm suspension was spotted onto 0.1% poly L-lysine pre-coated slides (Thermo Scientific). After attachment ...
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bioRxiv - Molecular Biology 2021Quote: RNA-FISH experiments were performed using the ViewRNA ISH Cell Assay (ThermoFisher, QVC0001) protocol with minor modifications ...