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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate (2 mg/mL in 200 μL) was precleared with 20 μL of protein Pierce protein A/G magnetic beads (Thermo Fisher, 88802). 5 μg of anti-KEAP1 (Santa Cruz Biotechnologies ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer was achieved using the iBlot™ 2 Gel Horizontal Transfer Device (Invitrogen). Nitrocellulose membranes were blocked in 5% BSA in PBS/Tween 20 (0.1% ...
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bioRxiv - Molecular Biology 2019Quote: ... Xpress-p85-A951E and Xpress-p85ΔRBS1/2 proteins were immunodetected using anti-Xpress (Invitrogen) antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated for at least 2 hours with Dynabeads Protein A (Invitrogen 10001D) rotating at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... bound protein complexes were eluted in 40 µl 2× LDS buffer (Thermo Fisher Scientific) and fractionated on 7% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane (iBlot™ 2 Transfer Stacks, ThermoFisher Scientific) blocked for 1h at RT with 5% Top-Block wt/vol in PBS supplemented with Tween-20 (10x PBS pH7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... The beads-protein complexes were isolated using a DynaMag-2 magnet (Thermo Fisher Scientific), applying 3 wash-resuspension cycles before short-term storage of the immunoprecipitate at −18 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred into a PVDF membrane (iBlot 2 Transfer Stacks, Invitrogen) and washed with Ponceau S red stain to confirm the successful transfer of proteins to the membranes ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Biochemistry 2022Quote: ... Bound proteins were eluted with 2 x SDS PAGE sample loading buffer (Thermo Fisher) and heating (95 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was eluted using 2 µg/mL HA peptide (Thermo Scientific, 26184) in 50 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto a PVDF membrane using the iBlot-2 transfer system (Invitrogen). The membrane was washed and blocked with 5% BSA in Tris buffered saline with 0.05% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-sarcomeric protein α-Actinin 2 and ProLong Gold Antifade were purchased from Thermofisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to PVDF membrane using dry transfer method via iBlot 2 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μg pCMV-VSV-G envelope and 0.5 μg pGP packaging plasmids using Lipofectamine 3000 transfection reagent (Invitrogen, Carlsbad, CA, US) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... the PSG3 plasmid was co-transfected into HEK293T cells along with JRFL or YU2 HIV envelope plasmids using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cancer Biology 2022Quote: ... Transient retroviral supernatants were generated by transfecting the vector encoding the TCR of interest (MSGV1) and envelope (RD114) into 293GP cells using Lipofectamine 2000 (Life Technologies). PBLs were activated in T cell medium supplemented with 50 ng/mL anti-CD3 and 300 IU/mL IL-2 for 3 days before retroviral transduction ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Physiology 2019Quote: ... Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific, #1856209). Cell lysate was incubated with Anti-FLAG M2 antibody for 1 h and immunoprecipitated (IP ...
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bioRxiv - Bioengineering 2023Quote: ... was used for separation of the proteins by molecular weight and then separated proteins were transferred in iBot 2 PVDF membrane (Thermo Fisher Scientific, USA). Anti-Epo polyclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
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bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...