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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Baculoviruses were amplified twice and freshly generated P3 virus was used to infect 1.8-2 L of High Five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 6 mL virus per 600 mL of cells ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded along with the SeeBlue Plus 2 protein marker (LC5925, Life Technologies). The gel was run at 60V for 20 mins ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Microbiology 2020Quote: ... or P1 SARS-CoV-2/München- 1.1/2020/929 (Munich) virus was diluted to 500 µl in 15 ml with virus dilution medium (Opti-MEM, Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Next day cells were washed with phosphate-buffered saline (PBS) and infected with virus in Virus Production Serum Free Media (VPSFM; Gibco) supplemented with 0.25-0.5 ug/mL TPCK-Trypsin ...
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bioRxiv - Immunology 2019Quote: ... and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen). Amplification of cDNAs was performed by quantitative real-time PCR reactions on a Light Cycler instrument (LC480 ...
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bioRxiv - Microbiology 2020Quote: ... Virus was diluted in infection media (DMEM (Invitrogen) supplemented with 35% BSA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Microbiology 2020Quote: ... and 50% virus solution in RPMI media (Gibco). Mosquitoes were left to feed for 1.5 h using Hemotek membrane feeder system (Discovery Workshops ...
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bioRxiv - Immunology 2021Quote: ... virus was diluted in RPMI-1640 media (Gibco) and maintained on ice ...
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bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
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bioRxiv - Cancer Biology 2023Quote: ... then virus pellets were suspended in HBSS (Gibco). The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara) ...
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bioRxiv - Genomics 2023Quote: ... infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Genetics 2024Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Neuroscience 2023Quote: ... Virus particles were resuspended in Neurobasal A (Invitrogen) and stored at -80 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
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The direction of local adaptation on pathogen exploitation rates reverses with environmental contextbioRxiv - Evolutionary Biology 2023Quote: ... We add 50 larvae that have orally ingested half their body lengths of virus solution to 2 25-cell compartmentalized square petri dishes (ThermoFisher Scientific, U.S.A.) with ‘standard’ food and incubate them for 20 days [61] ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 2 hours incubation with Protein A dynabeads (Invitrogen). Immunoprecipitated material was sequentially washed with buffer containing low salt (150 mM NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membranes using iBlot 2 (Invitrogen) and immunoblotting was performed as previously described in the western blot section.
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bioRxiv - Neuroscience 2019Quote: ... protein concentration was measured using Qubit 2 Fluorometer (Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: 293T cells were transfected with full-length envelope expressing plasmid using lipofectamine2000 (ThermoFisher, catalog no 11668027) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... pSPAX2 packaging vector and pVSVG envelope vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) as described (73) ...
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bioRxiv - Cell Biology 2019Quote: ... and the lentiviral envelope vector VSVg were transfected into HEK 293T cells with TurboFect (Life Technologies). Transfected HEK 293T cells were incubated for two days before the virus-laden medium was transferred to HMECs or K562 cells ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Biophysics 2023Quote: ... and a BG505 envelope SOSIP were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). Before transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was detected by virus-specific primer/probe according to indicated reference sequences (Table 1) utilizing TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems) and QuantStudio™ 5 (ThermoScientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...