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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-goat RFC1 antibody (2 μg/sample) coupled to Protein G agarose beads (Thermo Fisher Scientific) for 2 h on an end-over-end rotator at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were transferred to the PVDF membrane by using iBlot™ 2 Transfer Stacks (Thermo Fisher Scientific) and iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific, USA). Samples were then reduced using DTT (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins in the supernatant were reduced with 25 mM tris(2-carboxyethyl)phosphine (TCEP, Thermo Scientific, #20490) for 1 hour at RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... The proteins were transferred from the gel to a nitrocellulose membrane using a iBlot 2 device (ThermoFisher) and the membrane was immunostained using an iBind instrument (ThermoFisher) ...
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bioRxiv - Bioengineering 2021Quote: ... and probed with SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher # MA5-35946) and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals) ...
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bioRxiv - Cancer Biology 2022Quote: ... The RNP complex was assembled at 1:2 molar ratio of TrueCut™ Cas9 Protein v2 (Invitrogen) to synthesised gRNA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a PVDF membrane using iBlot 2 transfer system (Thermo Fisher Scientific IB24001). Membrane was blocked in 5% Thermo Scientific™ Oxoid™ Skim Milk Powder (Fisher Scientific OXLP0031B ...
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bioRxiv - Microbiology 2019Quote: ... Reacted protein bands were visualized by using Pierce™ ECL 2 Western Blotting Substrate (Thermo scientific, USA) and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... TEV protease (a gift from Dirk Görlich) and Histone1-Alexa-488 protein (2 mg/ml, Thermo Fisher) were injected to the desired embryos using Microinjector FemtoJet® (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was finally buffered with PBS and quantified using Qubit 2 Fluorometer (Thermo Fisher Scientific), and stored at −80 °C until use.
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bioRxiv - Immunology 2021Quote: ... or B.1.351 Spike protein (Table 2) were produced using HEK293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
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bioRxiv - Cell Biology 2022Quote: ... was followed by protein transference to PVDF membranes using an iBlot 2 transfer system (Thermo Fisher Scientific). Membranes were incubated for 45 min in Rockland blocking buffer (Rockland ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were reduced and alkylated by adding tris(2-carboxyethyl)phosphine (TCEP) (77720, Thermo Scientific, United States) and iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to PVDF membranes using iBlot 2 dry blotting system (Thermo Fisher Scientific Cat# IB21001). Primary antibodies were used at concentrations recommended by the vendor (see Supplementary Table 3 for antibody information) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... #2 or IgG (rabbit; self-made) were coupled per µl magnetic Protein G dynabeads (Life Technologies 10004D) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred onto an iBlot membrane using an iBlot 2 Western Blot Transfer System (Thermo Fisher). Blots were incubated in skim milk solution (50 g/L skim milk powder in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane using the Invitrogen iBlot 2 transfer device (ThermoFisher: IB21001), blocked for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred onto nitrocellulose membranes using an iBlot™ 2 Dry Blotting System (Thermo Fisher Scientific). Total protein profile on the membranes was stained using Revert™ 700 Total Protein Stain following instruction (LI-COR ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentiviruses were obtained by transient transfection of the psPAX2 packaging plasmid and pMD2.G envelope plasmid in HEK293T cells by using Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA). Transfection efficiency was validated by western blotting ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Catalog Numbers 4444432). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from purified virus using the GeneJET genomic DNA purification kit (Thermo Fisher), and the fidelity of the changed regions was verified by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... wt Flock House virus (FHV) was generated by transfecting S2 cells with pMT plasmid vectors (Invitrogen) containing respective genomes (NC_004146 for RNA 1 ...
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bioRxiv - Biochemistry 2019Quote: ... 293FT producer cells with ViraPower packaging mix (pLP1, pLP2, and pLP/vesicular stomatitis virus; ViraPower; Invitrogen) to generate the lentivirus according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times and supplemented with virus production serum-free media (VP-SFM, Gibco) containing 0.6 µg/ml trypsin ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Clarified virus working stock supernatant was overlaid onto a 25% sucrose-NTE (100nM NaCl (ThermoFisher Scientific), 10mM Tris (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reprogramming to pluripotency was carried out using the CytoTune 2.0 sendai virus kit (Thermo-Fisher Scientific) to overexpress four transcription factors (OCT4 ...
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bioRxiv - Microbiology 2020Quote: ... unbound virus was removed by washing three times with PBS (Life Technologies, Carlsbad, California, United States). Next ...
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bioRxiv - Microbiology 2020Quote: ... The virus in the growth medium was recovered by Polyethylene Glycol 8000 (PEG-8000, Fisher Scientific) precipitation ...
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bioRxiv - Biophysics 2020Quote: Virus was attached to a gold-coated probe using dithiobis succinimidyl propionate (DSP) linkers (Thermo Scientific). Dithiobis succinimidyl propionate (DSP ...
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bioRxiv - Cell Biology 2020Quote: ... and sendai-virus iPSCs were cultured on matrigel-coated plates (Geltrex™ LDEV-Free, ThermoFisher Scientific) in Essential 8™ medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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bioRxiv - Genomics 2021Quote: ... we reprogrammed fibroblast samples from 139 iPSCORE individuals using non-integrative Cytotune Sendai virus (Life Technologies) and the 149 iPSCs (7 subjects had 2 or more clones each ...