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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Virus (undiluted) or FluoSpheres (580/605, 0.04 µm, diluted to 20% in saline, Invitrogen) were loaded into freshly pulled glass micropipettes and injected using a Nanoject III pressure injector (Drummond Scientific Company ...
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bioRxiv - Microbiology 2022Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Virus amplification was carried out in Spodoptera frugiperda Sf9 cells (Geneart, Thermo Fisher Scientific) and was used to infect Trichoplusia ni Tnao38 cells (103 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed using Murine Moloney Leukemia Virus Reverse Transcriptase (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) and real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cellular RNA was mixed with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems), 10 mM forward and reverse primers and 2 mM probe ...
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bioRxiv - Immunology 2023Quote: ... medium containing virus isolates was incubated in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11995073) with 1:10000 Vybrant (V22887 ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Yamanaka reprogramming factors were induced to iPSCs via Sendai virus using CytoTune2.0 (ThermoFisher, A16517). To establish nGFP+ iPSCs ...
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bioRxiv - Genetics 2023Quote: ... Virus particles were subsequently washed multiple times with PBS (pH 7.4, Thermo Fisher Scientific) and physical titers were measured using a Qubit 3.0 fluorometer and the Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and N gene-specific probe and reverse primer as previously described47:
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to polyvinylidene difluoride membranes using the iBlot 2 dry blotting system (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes using an iBlot 2 Dry Blotting System (Invitrogen, Waltham, MA) set to 20V ...
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bioRxiv - Genetics 2019Quote: ... proteins were transferred to a nitrocellulose membrane using the iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in 1X TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µl of the studied protein sample was loaded onto a zeroed NanoDrop spectrophotometer (Thermo Scientific). The absorption spectra were recorded in the range of 220-350 nm.
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bioRxiv - Biophysics 2021Quote: ... Protein was then transferred to a PVDF membrane using iBlot™ 2 Transfer Stacks (Invitrogen IB24002) in iBlot 2 dry blotting device (Invitrogen IB21001) ...
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bioRxiv - Microbiology 2020Quote: ... anti-NeuN and anti-SARS-CoV-2 spike protein antibodies (Thermo Fisher Scientific, Norcross, GA, USA) overnight at 4°C followed by incubation with Alexa Fluor 546- or Alexa Fluor 488-conjugated secondary antibody for 1 hr at room temperature [31,34] ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred from the gel using the iBlot® 2 transfer stacks (Life Technologies, U.S.A) using the iBlot® Gel Transfer Device (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred onto a PVDF membrane using the iBlot™ 2 Transfer System (IB24001, Invitrogen). Membranes were blocked with 3% milk/TBST (T8793 ...
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bioRxiv - Immunology 2022Quote: ... followed by a 2 h treatment in T-PER™ Tissue Protein Extraction Reagent (Thermo Scientific) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... each protein pellet was digested with 2 µg sequencing grade trypsin (Thermo Scientific, Rockford, IL, USA) overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of the purified BCL-2 family proteins was determined by BCA assay (ThermoFisher Scientific), and they were flash-frozen in liquid nitrogen and stored at -80⍛C.
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bioRxiv - Biochemistry 2021Quote: Protein gels were either dry transferred onto nitrocellulose membranes using the iBlot™ 2 device (Invitrogen) or wet transferred onto PVDF membrane using the Mini Trans-Blot™ Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated SARS-CoV-2 S1 and RBD proteins were combined with fluorescent NeutrAvidin beads (Life Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were blotted using an iBlot 2 Dry Blotting System (Thermo Fisher Scientific, Inc., Erlangen, Germany) to a polyvinylidene difluoride membrane (GE Healthcare Europe GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes using an iBlot 2 dry blotting system (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher), heating to 95 °C for 10 min and running on an SDS-PAGE gel ...
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bioRxiv - Bioengineering 2022Quote: ... Separated proteins were transferred onto polyvinylidene difluoride (PVDF) membranes using an iBlot 2 (Thermo Fisher Scientific) semi-dry transfer system ...
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bioRxiv - Synthetic Biology 2023Quote: ... Proteins in the gels were transferred to PVDF membranes using the iBlot 2 transfer device (Invitrogen) and corresponding protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and proteins were transferred to PVDF membranes using the iBlot 2 transfer system (Thermo Fisher Scientific). Membranes were incubated for 1 hr in Rockland blocking buffer (Rockland ...
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bioRxiv - Immunology 2023Quote: ... Protein A/G magnetic beads were incubated with 2 μg anti-p-JAK2 (44-426G, Invitrogen), anti-IRE (NB100-2324 ...
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bioRxiv - Systems Biology 2023Quote: ... Separated proteins were transferred onto an iBlot™ 2 Transfer Stacks PVFD membrane (Invitrogen™ IB24002) using dry transfer (as described previously [53] ...
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bioRxiv - Cancer Biology 2023Quote: ... and captured proteins were eluted by boiling beads in 2× NuPAGE LDS Sample Buffer (Life Technologies) containing 100 mM DTT for 45 minutes at 95°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The electrophoresed proteins were transferred to PVDF membranes using iBlot-2 dry blotting system (Thermo Fisher). The following primary antibodies were used ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants were first precleared for 2 h at 4°C using Protein G Dynabeads (Invitrogen). Precleared samples were immunoprecipitated with anti-H3K9me3 (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was then transferred to PVDF membranes using the iBlot 2 Dry Blotting System from Invitrogen. The membrane was then used for western blot detection in one of two ways.
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bioRxiv - Immunology 2023Quote: Recombinant Omicron RBD proteins from variants BA.2 and BA.4.5 were obtained from Fisher Scientific. To determine the specificity of mAbs ...
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bioRxiv - Immunology 2023Quote: ... added to 2 μL of TruCut Cas9 Protein v2 (5 ng/μL, Thermo Fisher Scientific A36498), and incubated for 10 minutes at room temperature ...