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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Final protein concentration was assessed using 5,5-dithio-bis-(2-nitrobenzoic acid) (ThermoFisher) and a standard curve of glutathione (Aitken & Learmonth ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were reduced with 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes using the iBLOT 2 system (Life Technologies) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... Two (2) mL was used to quantify the protein concentration with Qubit (Invitrogen) reagent according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... then for an additional 2 hours with magnetic Dynabeads Protein A (Thermo Fisher). Protein complexes were isolated ...
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bioRxiv - Plant Biology 2021Quote: ... After an additional 2 h incubation with Protein G Agarose (20399, Thermo Scientific), beads were washed with wash buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... 2% SDS) and protein concentration was determined with BCA assay kit (Thermo Fisher) using BSA suspended in Leammli buffer as standard ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Immunology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes using the iBLOT 2 system (Life Technologies) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Separated proteins were transferred onto iBot 2 PVDF membrane (Thermo Fisher Scientific, USA). Anti-Epo polyclonal antibody (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were reduced with 5 mM tris (2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90 °C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using 48 pairs of in-house DENV serotype-specific primers and the SuperScript III One-Step RT-PCR system with Platinum® Taq High Fidelity polymerase (ThermoFisher Scientific, MA, USA), followed by cDNA purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant (2 mg of proteins) was incubated with 2 μg of target antibodies and 20 μl of Protein A/G Plus Ultralink Resin (Thermo Scientific) for 4 hours at +4°C ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... the cloned plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Invitrogen) based on the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5 μg psPAX2 packaging plasmid and 500 ng pMD2.G envelope plasmid were co-transfected in HEK293 FT cells in 10-cm dishes using Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the packaging construct pCMVD8.9 and the envelope coding plasmid pCMV-VSVG (from Dr. Soosan Ghazizadeh, SUNY Stony Brook) into 293T cells using lipofectamine 2000 (Invitrogen). Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 293GP cells were then transfected with 6 μg of a candidate TCR pMSGV1-plasmid in the presence of the 3 μg of RD114 envelope using Lipofectamine 3000 (Invitrogen). The pMSGV1-plasmid and RD114 were obtained through an MTA from S ...
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bioRxiv - Microbiology 2022Quote: ... Viral stock was generated by co-transfecting HEK 293T cells with 10:1 ratio of HIVGKO plasmid and a plasmid encoding VSV-G envelope using Lipofectamine 3000 reagent (Invitrogen). Culture supernatant was harvested 48hrs post-transfection ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... together with psPAX2 packaging and pMD2.G envelope plasmid DNA were co-transfected to HEK293T cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and envelope (pMD2.G; 0.5 µg) plasmids using either FuGENE 6 or Lipofectamine 3000 transfection reagent (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T was co-transfected with lentiviral expression constructs (12 µg), viral packaging plasmid (psPAX2, 8 µg), and viral envelope plasmid (pMD2.G, 4 µg) using Lipofectamine 2000 (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Molecular Biology 2021Quote: ... We used TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher Scientific) to set up 10 μl (half volume ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... c-MYC and KLF4 in CytoTune Sendai virus vectors (Thermo Fisher Scientific) (Sontag et al. ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...