Labshake search
Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
-
bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
-
bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
-
bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
-
bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
-
bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
-
bioRxiv - Microbiology 2021Quote: ... that had been previously labeled with Alexa-488 (anti-serotype 4-Alexa-488, to stain TIGR4) or Alexa-555 (anti-serogroup 19-Alexa-555, to stain GA47281) (Molecular Probes). Stained preparations were finally washed two times with PBS ...
-
bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were eluted in 2× NuPAGE LDS Sample Buffer (Life Technologies) by boiling at 95 °C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
-
bioRxiv - Molecular Biology 2021Quote: ... pre-cleared for 2 h with Protein A Dynabeads (Invitrogen, 10001D), followed by overnight incubation with 5 µg of antibody (anti-SKIV2L Proteintech group ...
-
bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
-
bioRxiv - Cell Biology 2020Quote: ... Fura-2 BZiPAR and Protein G magnetic beads were from Invitrogen - Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Physiology 2021Quote: ... mouse-anti Microtubule-associated protein 2 (MAP2) (Invitrogen, Cat # 13-1500) (1:1000) ...
-
bioRxiv - Neuroscience 2020Quote: ... lysosome-associated membrane protein 2 (LAMP2; 1:1000; Thermofisher, Waltham, MA), or phopsho-ribosomal S6 protein (PS6 ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein bands were revealed using ECL reagent (ECL 2, Thermo Scientific) with a Typhoon 9419 phosphorimager (GE Healthcare ...
-
bioRxiv - Neuroscience 2023Quote: ... supernatant proteins (2 mg/ml) were reduced with 25mM TCEP (ThermoFisher) for 1 h at 55°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific, Loughborough, UK) was used ...
-
bioRxiv - Microbiology 2019Quote: ... with TaqMan fast virus 1-step master mix (Thermo scientific) using AriaMx instrumentation (Agilent).
-
bioRxiv - Cancer Biology 2019Quote: ... Virus was then resuspended in 1:1 Opti-MEM (Gibco) - HBSS ...
-
bioRxiv - Microbiology 2019Quote: ... fibroblast-derived virus stocks were treated with TurboDNase (Thermo Fisher). For this ...
-
bioRxiv - Immunology 2021Quote: ... Pelleted virus was resuspended in Opti-MEM (Gibco 31985-070) and stored at −80 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The virus pellet was resuspended in 1 mL PBS (Gibco). For long term storage ...
-
bioRxiv - Cell Biology 2021Quote: ... In brief the virus was diluted in DMEM (ThermoFisher Scientific) to achieve a multiplicity of infection (MOI ...
-
bioRxiv - Pathology 2022Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix were used ...
-
bioRxiv - Molecular Biology 2020Quote: ... and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) with 250 μM deoxy nucleoside triphosphate (dNTP ...
-
bioRxiv - Microbiology 2019Quote: ... and Moloney murine leukemia virus reverse-transcriptase (MMLV-RT, Invitrogen). Subsequent PCR amplification performed in a thermocycler using specific primers ...
-
bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
-
bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
-
bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
-
bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...
-
bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
-
bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
-
bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
-
bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific), primers ...
-
bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
-
bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
-
bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
-
bioRxiv - Microbiology 2023Quote: We initially obtained baculovirus carrying the genetic information of full-length rhesus rotavirus VP7 (G3 serotype) from bacmid transfected Sf9 cells (Thermo Fisher Scientific). Sf9 cells were then inoculated with baculovirus and passaged in the same cells three times ...
-
bioRxiv - Microbiology 2022Quote: ... envelope plasmid (0.9 µg pMD2.G) and pLX304 carrying eGFP-LC3B (9 µg) using Opti-MEM (Fisher Scientific) and FuGENE HD transfection reagent (Promega) ...
-
bioRxiv - Cell Biology 2023Quote: ... 6ug of psPAX2 viral packaging vector and 3ug pMD2.G viral envelope vector overnight with Lipofectamine 3000 (Invitrogen) according to manufacturer protocol ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
-
bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
-
bioRxiv - Genetics 2023Quote: ... we incubated 2-5μg sheared DNA with equal volume of DynabeadsTM Protein A and Protein G (ThermoFisher Scientific Inc.). ChIP-seq libraries were prepared with a NEBNext DNA Ultra II kit following manufacturer recommendations ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were blotted onto nitrocellulose using the iBlot™2 (Thermo Fisher) and developed using LiCor Odyssey CLx Infrared Imaging System.
-
bioRxiv - Molecular Biology 2021Quote: ... overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen). The beads were washed 3 times with buffer (10mM TRIS-HCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2×106 cells and 50 µg of Protein A Dynabeads (Life Technologies) were used per IP ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced with 5mM Tris(2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
-
bioRxiv - Plant Biology 2019Quote: ... Proteins were detected using chemiluminescent Pierce ECL 2 substrate (Thermo Fisher Scientific) and imaged using Azure Imager c400 (Azure Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: ... overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen). The beads were washed 3 times with buffer (10mM TRIS-HCl ...