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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... USA) dissolved in ethanol or 1μL 2.5mg/mL N,N-Dimethyl-6-dodecanoyl-2-naphthylamine (LAURDAN; Thermo Fisher, USA) dissolved in dimethyl sulfoxide to label the EVs ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Genomics 2024Quote: ... and the relative expression was determined by the 2-ΔΔCt method on a QuantStudio 6 qPCR machine (Thermo Fisher). The levels of mRNAs were normalized to human ACTB.
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bioRxiv - Neuroscience 2024Quote: Brains were dissected from 2-day old adults in PBS and incubated in 1.6 × 10-6 M acridine orange (ThermoFisher) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... equal amounts of RNA were prepared in equal volumes (6 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Microbiology 2024Quote: ... sg.ORF7a) and genomic RNA (NSP3 region; g.NSP3) (90) on a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). Relative mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Minimum triplicate reactions per sample were performed using a QuantStudio 6 flex qPCR machine (Applied Biosystems, Foster City, CA, USA) according to the following thermal cycling conditions ...
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bioRxiv - Cancer Biology 2021Quote: Cancer cells were transfected at ∼30% confluency in 6-well plates with 5nM control or target siRNA using Lipofectamine RNAiMAX (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... T175 flasks containing intracellular parasite grown in normal media were treated with auxin for 6 hours before sample collection and addition of Trizol (Invitrogen). RNA was extracted as per manufacturer instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS as previously described (Qiang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Developmental Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... aureus overnight cultures were diluted 1:200 into 3ml of fresh TSB or TSBg per well of a Nunc MicroWell 6-well plate (no. 140675; ThermoFisher) and incubated statically at 37°C for 6 hours ...
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bioRxiv - Microbiology 2019Quote: N2a cells were transiently transfected at ≈70% confluence in a 6-well plate by adding per well 10 μL Lipofectamine 2000 (Invitrogen) together with 3 μg of the respective pcDNA3.1 plasmid derivative ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from the 60 hpa tissues (n=6) from each group using TRIzol reagent (Applied Biosystems, USA). One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: Splenocytes from C57BL/6 mice were disassociated into a single cell suspension and labeled with 1μM CellTrace Far Red (ThermoFisher Sci). Targets were then divided into two populations ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Bioengineering 2022Quote: hPSC-CMs were maintained in Matrigel pre-coated 6-well plates in RPMI 1640 medium (Gibco REF 11875-093 500ml) with B-27 supplement (Gibco REF 17504-044 10ml) ...
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... after collection at Day 6 and resuspended to the concentration of 0.2 × 106 cells/mL with MN medium consisting of Neurobasal medium (Gibco 21103049) with 1× B27 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Immunology 2022Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of vector and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). Virus supernatants were collected 48 h ...
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bioRxiv - Immunology 2021Quote: Multiplex quantitative polymerase chain reaction (qPCR) was carried out using a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously [23] ...
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bioRxiv - Immunology 2021Quote: ... and KP cells (lung adenocarcinoma; C57BL/6 mice) (Xue et al., 2011) were grown in IMDM with 10% heat-inactivated FBS (GIBCO), 100 U/ml penicillin/streptomycin (GIBCO ...
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bioRxiv - Immunology 2019Quote: Skin samples were collected from the cheek of mice at the indicated time points with a 4- or 6-mm biopsy punch into cold RPMI 1640 medium (RPMI; Gibco) and minced into smaller pieces with surgical scissors ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...
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bioRxiv - Microbiology 2019Quote: ... 6 μg of total RNA was incubated with 1.4 μg of anchored Oligo(dT)20 primer (Invitrogen, Carlsbad, CA, USA) in a total volume of 18.5 μl for 10 min at 70°C ...
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bioRxiv - Bioengineering 2019Quote: ... Primary neurons were then prepared and cultured on mirrors in a 6-well culture dish based on the protocol provided by ThermoFisher. All the laser measurements were conducted using the neurons 3 days after seeding.
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bioRxiv - Bioengineering 2019Quote: ... The bionic corals were transferred to 6-well plates filled with 3 mL of ASW medium30 containing broadband antibiotics (penicillin/streptomycin, Gibco) at a concentration of 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Separated proteins were blotted onto nitrocellulose membranes at 20 V for 6 minutes using an iBlot system (Invitrogen, Carlsbad, CA). After transfer ...
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bioRxiv - Plant Biology 2019Quote: ... The respective antibodies or IgG were coupled for 4-6 hour to Protein G Dynabeads (50µl, 10004D, Thermo Fisher Scientific) and subsequently incubated overnight with equal amounts of sonicated chromatin ...
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bioRxiv - Cancer Biology 2019Quote: ... were seeded in 6-well plates at a density of 300,000 cells per well in RPMI-1640 (Thermo Fisher Scientific) with 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF7 cells expressing either SNHG7 isoform or vector alone were created by cloning and then transfecting (Fugene 6) the respective SNHG7 isoform from MCF7 generated cDNA using the GeneRacer Kit (Thermofisher) after 3’ RACE (see Supplementary Methods for primers and additional details) ...
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bioRxiv - Developmental Biology 2019Quote: ... E8 medium was replaced daily and hiPSC were gently passaged every 5-6 days by mechanical selection or bulk passaged using non-enzymatic reagents (i.e., Versene solution (ThermoFisher Scientific) or Phosphate-Buffer-Saline (PBS)-based enzyme-free cell dissociation buffer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Cell Biology 2019Quote: ... FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) was purchased from Invitrogen. Rapamycin was purchased from LC Laboratories ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded on 25 mm coverslips in a 6-well dish and incubated with 10 μM BrdU (Invitrogen) for 12 to 24h ...