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Citations for Thermo Fisher :
2801 - 2844 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Tissues were frozen in OCT (Sakura, #4583) and 8 µm sections were prepared by cryostat on SuperFrost Plus Gold slides (Fisher Scientific, #15-188-48). Sections were permeabilized with 1X permeabilization buffer (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Immunology 2023Quote: ... The filtered supernatants were concentrated 8-fold using a pre-rinsed 3K MWCO Pierce Protein Concentrator PES (molecular-weight cutoff: 3 kDa) (Thermo Fisher Scientific, 88525, USA) by centrifuging at 4000 rcf in 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transduced with lentivirus in media containing polybrene (8 µg/ml) for 24 hours followed by antibiotic selection with 10 µg/ml Blasticidin (Thermo Fisher Scientific, Cat# A1113903). Non-transduced cells were included as controls ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... the dried pellet was resuspended in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). A 2 hour incubation with 10 mM DTT at 56 °C was followed by 40 mM iodoacetamide for 45 minutes in the dark at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... were seeded separately at a 70% confluency in 8-wells chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™). After 24h of incubation ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with cross-linking buffer (20 mM sodium phosphate [pH 8], 150 mM NaCl) supplemented with 1.2 mM bis(sulfosuccinimidyl)suberate (BS3, Thermo Fisher Scientific catalog number A39266) for 30 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... were seeded separately at a 70% confluency in 8-wells chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™). After 24h of incubation ...
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bioRxiv - Bioengineering 2023Quote: MDA-MB-231 cells were plated in an 8-well chamber (1 × 104 cells/well) (Nunc™ Lab-Tek™ II chambered cover glass) and cultured for three days ...
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bioRxiv - Neuroscience 2023Quote: ... CFA was prepared by adding 8 mg/ml of heat– killed Mycobacterium tuberculosis H37 Ra (Difco, catalog no. 231141) to incomplete Freund’s adjuvant (Thermo Fisher Scientific, catalog no. 77145). MOG35-55 peptide (2 mg/ml in PBS ...
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bioRxiv - Neuroscience 2024Quote: L3-L5 DRGs from male and female 8-wk- or 21-wk- old mice were collected into cold dissection medium [HBSS (Thermofisher, Gibco; Catalog#:14175-079)] with 10% 1M HEPES (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... and U-2 OS cells were grown in 4-well or 8-well Nunc™ Lab-Tek™ II Chambered Coverglass (Borosilicate Glass 1.5; Thermo Fisher Scientific, 155360) coated with poly-L-lysine ...
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bioRxiv - Neuroscience 2024Quote: ... 12 μl of the master mix and 8 μl of RNA samples were added into MicroAmp PCR tubes (Applied Biosystems, Thermo Fisher Scientific, USA), followed by microcentrifugation at a low speed for 5 s ...
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bioRxiv - Neuroscience 2024Quote: ... 12 μl of the master mix and 8 μl of RNA samples were added into MicroAmp PCR tubes (Applied Biosystems, Thermo Fisher Scientific, USA), followed by microcentrifugation at a low speed for 5 s ...
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bioRxiv - Systems Biology 2024Quote: ... Metabolite separation was performed through a HyperREZTM XP Carbohydrate H+ 8 mm column fitted with a HyperREZTM XP Carbohydrate Guard (Thermo Fisher Scientific, Waltham, MA). The mobile phase consisted of 1.5 mM of H2SO4 at a flux of 0.6 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... 10µl of the deacylated tRNA-enriched RNA samples were mixed with 8 µl of 2X RNA loading dye (Thermo Scientific™ Cat No. R0641), denatured for 3 min at 72°C and the whole volume was loaded onto the gel ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IPSCs were maintained on human Laminin-521 (BioLamina, Sundbyger, Sweden) coating in essential 8 (E8) medium (DMEM/F12 supplemented with 15 mM Hepes (Gibco/Fisher Scientific, Hampton, New Hampshire, USA), 16 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... They were dissociated and centrifuged as described above and pelleted cells were resuspended in Day 0 differentiation medium containing the following: Essential 8™ Basal Medium (Gibco; Cat. No. A15169-01) as a base ...
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bioRxiv - Physiology 2019Quote: ... 1 μL of a FAM-labeled MT-ATP 8-probe or 1 μL of a MVIC-labeled GAPDH-probe (both probes from Applied Biosystems, Branchburg, New Jersey, USA), and 2 μL of TE buffer containing cf DNA isolated from plasma ...
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bioRxiv - Microbiology 2019Quote: ... pellets were thawed at 37°C and then subjected to a single round of sonication on ice (Sonic Dismembranator 60, Fisher Scientific, setting 8, 5 seconds). Lysates were clarified by centrifugation at 20,000 rcf for 60 minutes at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... Finally the obtained bacterial lysate was clarified by centrifugation at 21,000 rpm for 30 minutes (Thermo Lynx 4000, A27-8 rotor, Thermo Fisher Scientific, Waltham, MA, United States). The supernatant was incubated with 2 mL TALON resin (Takara ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed 3 times for 8 minutes each and then placed in the secondary antibodies conjugated to either Cy3 or FITC (Thermo Fisher Scientific, Waltham, MA, USA) at a dilution of 1:1000 in 1% BSA for 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... A flow rate of 0.2 ml per minute was maintained on a 60 min gradient using mobile phase A (10mM ammonium bicarbonate, pH 8, Fisher Scientific, cat. no. BP2413-500). Elution was with mobile phase B (acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... we added 100 µl of master mix containing 8 U DNase I (TURBO DNA-free kit, catalog no. AM1907, Thermo Fisher Scientific, Waltham, MA, USA), 1X DNase I buffer ...
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bioRxiv - Microbiology 2020Quote: Purified PFABN was suspended in water at a concentration of 1 mg/mL and transferred to 8 mm crimp top glass vials (Fisher Scientific; Cat. No.: C4008-632C). 175 μL of 2 M trifluoroacetic acid containing 15 ng of D6-myo-inositol was added and the vials were capped with Teflon-coated aluminum caps (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Serial block-face imaging was performed at 1.8 KeV and 800 pA using the ICD and TLD detector in backscattered mode and the ASV 4 software (Thermo Fisher Scientific, Hillsboro, Oregon, USA). The block was milled at a current of 750 pA with 10 nm slices and images were acquired at a resolution of 10 nm/pixel with a dwell of 5 μs and a line average of 2 for a total z-depth of about 15 μm ...
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bioRxiv - Microbiology 2021Quote: ... we initially preformed biofilms by incubating 4×107 cells anaerobically at 37°C for 24 h in pre-reduced minimal medium (53) in a 25% saliva-coated well of an 8-well Chamber Slide System (Thermo Fisher Scientific, Waltham, MA, USA) with rotating ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Stock solutions of CuPT (50 mg/L) were prepared in the dark by dissolving CuPT (CAS# 14915-37-8, purchased from Acros Organics, now Thermo Fisher Scientific) powder in the nontoxic organic solvent dimethyl sulfoxide (DMSO ...
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bioRxiv - Genetics 2023Quote: Twenty μg protein samples were loaded into lanes of a 3-8% Tris-acetate gel (cat. EA0378BOX, NuPAGE™, Invitrogen, Thermo Fisher Scientific, CA, USA) and subjected to electrophoresis ...
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bioRxiv - Genetics 2023Quote: Twenty μg protein samples were loaded into lanes of a 3-8% Tris-acetate gel (cat. EA0378BOX, NuPAGE™, Invitrogen, Thermo Fisher Scientific, CA, USA) and subjected to electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: ... and blotted for 5 s with a blot force of 5 at ∼90% humidity and 8°C using a Vitrobot Mark IV (Thermo Fisher Scientific Inc., Waltham, USA) that was placed inside an anaerobic COY tent ...
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bioRxiv - Cancer Biology 2023Quote: Untreated MCF-7 and MDA-MB-231 cells were seeded at 2 x 104 cell per well on 8-well glass chamber slides (™ Nunc™ Lab-Tek™ II Chamber Slide™, Thermo Scientific). Cells were left to adhere for 24 hours in a 5% CO2 incubator at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 25-35ug nuclear extract was separated on home-made SDS-PAGE gels or NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies, for large Mediator subunits). Gels were blotted onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) (1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5ng cDNA and 8 pmol of each primer were used in each quantitative real-time polymerase chain reaction (Applied Biosystems Fast SYBR Green Master Mix, #4385612). Primer sequences were obtained from primer bank (https://pga.mgh.harvard.edu/primerbank/) ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Bioengineering 2022Quote: ... The 24-well plates with infected BHK-21 cells were overlaid with 1 ml of 0.8% methylcellulose in complete DMEM medium with 2% FBS and incubated for 5 days in a cell culture incubator (Thermo Fisher Scientific, 37°C and 5% CO2). Plaques were fixed and developed with staining reagent (1% crystal violet in 1:1 methanol/acetone solution ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs expressing six inducible transcription factors (MAFB, CEBPα, IRF, PU1, CEBPβ, IRF5) were grown in Essential 8™ Basal Medium (Gibco/Thermo Fisher Scientific, Cat. No. A15169-01), 10 μM ROCK inhibitor ...
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bioRxiv - Bioengineering 2023Quote: HGSOC cell lines (CaOV3, COV318, Kuramochi, OAW28, OVCAR4) were seeded in 8-well chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™) at a 70% confluency ...
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bioRxiv - Bioengineering 2023Quote: HGSOC cell lines (CaOV3, COV318, Kuramochi, OAW28, OVCAR4) were seeded in 8-well chamber slides (Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™) at a 70% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... with 50 ul transferred into each well of an 8-well chamber slide (Nunc™ Lab-Tek™ II Chamber Slide™ System, Thermo Scientific™, Massachusetts, United States). The chamber slide was then left open faced in a 37°C oven (Fisher Scientific ...