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Citations for Thermo Fisher :
2701 - 2750 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA of cells from cultures of 5-8 NT explants were extracted using the Ambion PureLink RNA Mini Kit (Invitrogen, cat. no. 12183018A) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... were cultured in suspension at 37°C in 8% CO2 shaking at 125 RPM in Freestyle F17 Expression Medium (GIBCO Cat# A13835-01) supplemented with 10% Pluronic F-68 and 200 mM of L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... The GMEM/KSR medium was consisted of 1 × Glasgow’s MEM (GMEM; Wako, 078-05525) supplemented with 8% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific, 10828-028). In the initial optimization experiments ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) was added on each pellet and total protein quantification was performed using BCA Protein Assay kit (Pierce™, Thermo Fisher Scientific). Before immunobloting analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells treated with the indicated concentrations of selected compounds for 72 h in 100 μL of incubation media were supplemented with 20 μL of 0.6 mM resazurin (Acros Organics 62758-13-8) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001, respectively). The cells were maintained so that once the iPSC colonies were confluent ...
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bioRxiv - Microbiology 2024Quote: Proteins were subjected to reduction in 4X reducing sample buffer (8% SDS, 400 mM Dithiothreitol) and resolved on a precast 4%–12% SDS-polyacrylamide gel (Thermo Fisher Scientific, #XP04125BOX). Coomassie staining was performed using Imperial Protein Stain (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and SDS-PAGE was performed with NuPAGE Novex 3 to 8% Tris-acetate gels in NuPAGE Tris-acetate SDS running buffer (Life Technologies Corp., USA) to resolve proteins >150kDa ...
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bioRxiv - Developmental Biology 2024Quote: ... POI-Halo organoids were seeded in 10-20 μL 90% Matrigel droplets into 8-well Labteks II #1.5 (Nunc, cat.# 12-565-338) and overlaid with 500 μL IntestiCult ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfections were done with 150 pmol of siRNA in complete DMEM medium for 8-10 hours using Lipofectamine™ RNAi max (ThermoFisher Scientific, 13778150). Media was changed following transfection incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar twisted pair platinum-iridium insulated electrodes (MS333/8-BIU/SPC, Plastics One, Roanoke, VA) coated with Vybrant® DiI cell-labeling solution (Invitrogen, Eugene, OR) were placed bilaterally in one of four regions of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos were mounted on a microscope slide with a Secure-Seal™ Spacer (8 wells, 9 mm diameter, 0.12 mm deep, Thermo Fisher Scientific, S24737), covered with a cover glass ...
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bioRxiv - Bioengineering 2024Quote: ... The electroporated sample was then transferred directly into one well of an 8-well microscopy slide (Nunc Lab-Tek II for widefield imaging) containing 300 µl prewarmed medium without antibiotics and was incubated for 24 hr in 5% CO2 atmosphere at 37 °C.
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bioRxiv - Neuroscience 2024Quote: ... (10 µl per mg tissue) After homogenizing with a Sonic Dismembrator (8 s on, 15 s off, three cycles in total, Fisher Scientific Model FB120), the mixture was centrifuged at 20,000 g (20 min ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 cells (Shanghai Cell Bank, Chinese Academy of Sciences, passages 3-8) were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... counted and replated at 80,000 cell/well on a 8-well microscope chamber slide (Nunc™ Lab-Tek II ™ Chamber Slide System, Cat# 154534PK Thermofisher Scientific) to prepare for infection experiments.
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bioRxiv - Bioengineering 2024Quote: ... Samples were sonicated on ice at 40% amplitude for one hour by a 120 W ultrasonicator with a 1/8” microtip probe (Fisher Scientific; Hampton, NH). Sonicated suspensions were ultracentrifuged at 58,000 x g for one hour using an Optima Max-XP Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Bioengineering 2023Quote: ... CytoTraceTM Red CMTPX pre-stained cells were cultured at 5 × 104 cells per well in 8-well confocal plate (Lab-Tek II Chambered Coverglass, 155409; Thermofisher Scientific, MA, USA) and incubated overnight at 37°C under 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Stock solutions of CuPT (50 mg/L) were prepared in the dark by dissolving CuPT (CAS# 14915-37-8, purchased from Acros Organics, now Thermo Fisher Scientific) powder in the nontoxic organic solvent dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Genetics 2022Quote: ... Purified PCR products were cloned into a GatewayTM entry vector using Invitrogen pCR™8/GW/TOPO™ TA Cloning (Invitrogen, catalog # K252002) followed by LR clonase™ reaction (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 or MCF-7 cultures were grown for imaging on poly-D-Lysine coated 8-well LabTek II chambered coverglasses (Nunc, Rochester, NY, USA) for 1-2 days ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Microbiology 2023Quote: ... ATCC CCL-244) and HCT-8 AhR knock-out cell lines (this study) were maintained in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL of suspension was settled on an 8-well diagnostic glass slide (Epredia™ - X2XER203B) and mounted with a #0 coverslip (Fisher Scientific – 10011913).
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bioRxiv - Biophysics 2023Quote: ... iPSCs were cultivated for several passages under feeder-free conditions in the Essential 8™ Medium on Geltrex LDEVFree hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific). 3–4 days before differentiation ...
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bioRxiv - Genomics 2023Quote: ... cat# HPSI0314i-cuhk_1) were maintained in a humidified incubator at 37°C and 5% CO2 using Essential 8 (Thermo Fisher Scientific, cat# A1517001) or Essential 8 flex (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tails were then placed in individual wells of 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA) with 200µl of Gibco CO2 independent medium (Thermo Fisher #18045054).
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bioRxiv - Biochemistry 2022Quote: ... and after adding extraction buffer (500 μL of 8M urea, 100 mM Tris, pH 8 and protease inhibitor cocktail; ThermoFisher Scientific; Cat# A32965) were sonicated at 30% amplitude with 3 cycles of 10 second pulses ...
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bioRxiv - Biochemistry 2023Quote: ... of centrifugation (12,000 rpm at 4 °C for 10 min)-cleared whole-cell lysate were loaded per lane in a commercial 3-8% 1.5-mm x 15-well Tris-Acetate SDS gel (Thermo Fisher Scientific, Catalog # EA03785BOX) with 1 x NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: mTERT-immortalized mouse CCD cells were cultured as described previously (8, 9) in 1:1 DMEM/F-12 (Gibco GlutaMAX™; Fisher #10565018) with 5% FBS (Gibco One Shot™ ...
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bioRxiv - Immunology 2023Quote: ... the debris spot was generated by adding 6 μL of the solution containing the necrotic hepatocytes into an 8-well chamber slide (Nunc, Rochester, NY, USA) and drying for 2 hours in the laminar flow ...
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bioRxiv - Immunology 2023Quote: ... We isolated CD4 T cells from pooled spleen and lymph nodes of 6-8-week-old Foxp3IRES-GFP mice using magnetic negative selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D), resting cells for at least 30 min at 4°C prior to electroporation ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μgs of centrifugation (12,000 rpm at 4 °C for 10 min)-cleared whole cell lysate were loaded per lane in a commercial 3-8% 1.5-mm x 15- well Tris-Acetate SDS gel (Thermo Fisher Scientific, Catalog # EA03785BOX), electrophoresing with 1 x NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The Aire exons 6 and 8 that were the targeted genomic regions were amplified through conventional PCR using PlatinumTM high fidelity Taq polymerase (Thermo Fisher, Cat # 11304011) and the oligonucleotide primers as mentioned above.
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis using 8 ng of total RNA was carried out using the High-Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, 4388950), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 24 WGS libraries were normalized to obtain a final concentration of 2 nM using 10 mM Tris-HCl (pH 8 or 8.5; Fisher Scientific, 50-190-8153). 0.5 to 1% PhiX library was spiked into the library pool as a positive control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of sample was injected and analyzed by LC-MS/MS using one quantifying and one qualifying ion for each compound (fentanyl and the internal standard) with a total run time ∼8 minutes but capable of multiplexing on a TLX4 (Thermo Fisher, Waltham, MA) 6500 mass spectrometer (ABSciex ...
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bioRxiv - Microbiology 2023Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a final concentration of 0.2 mg/ml followed by a 10-min incubation and washing with imaging buffer (IMB; 12 mM HEPES pH 8 (Thermo Fisher Scientific, 15630056), 40 mM Tris pH 7.5 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... qPCRs were performed using cDNA (1:8 dilution) and the ORA™ SEE qPCR Green ROX L kit (highQu) on a PikoReal 96 machine (Thermo Fisher Scientific). GraphPad Prism was used for graphics/statistics ...
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bioRxiv - Microbiology 2023Quote: ... The following day cells were centrifuged at 2,000 x g for 10 minutes and the cell pellet was lysed using 8 mL of B-PER Bacterial Protein Extraction Reagent (Thermo Scientific, Waltham, MA) per gram of pellet with gentle shaking for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... A portion of labelled and unlabeled lipoprotein samples were analyzed by SDS-PAGE on NuPAGE 3-8% gradient Tris-Acetate gels (Thermo Fisher Scientific, # EA0375) under reducing and nonreducing conditions followed by SilverQuest Silver Staining (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: L3-L5 DRGs from male and female 8-wk- or 21-wk- old mice were collected into cold dissection medium [HBSS (Thermofisher, Gibco; Catalog#:14175-079)] with 10% 1M HEPES (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... The BDB was homogenized at 15 Hz speed with 1.25 mm beads for 8 min in a Tissuelyzer (Thermo Fisher scientific, MA, USA) in 1mL TriZol prior to extraction ...
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bioRxiv - Cancer Biology 2021Quote: ... The media in 8 chamber imaging slides containing tumor cell-collagen matrices was replaced with Gibco RPMI 1640 with no phenol red (Thermo Fisher Scientific, MA, USA) with 10% Gibco fetal calf serum (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: For the cells cultured in the wells of the 8-well Nunc™ Lab-Tek™ II chamber slide (Thermo Fisher Scientific, Rochester, NY), the cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and then permeabilized using 0.1% Triton-100 for 10 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... 3×104 hTERT-RPE cells expressing mKO2-PACT were seeded into 8 well glass bottom dishes (Nunc™ Lab-Tek™ II Chambered Coverglass, Thermo Fisher Scientific) and cultured overnight ...