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Citations for Thermo Fisher :
2251 - 2300 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: MSOD-B cells and their modified progeny were harvested from culture flasks by adding Trypsin-EDTA (0.25%) (Gibco) and subsequently seeded on cylindrical Collagen Type I scaffold (AviteneTM UltrafoamTM Collagen Sponge ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were plated at 20% confluence in complete DMEM supplemented with 150 μg/mL hygromycin B (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were gavaged with a 2% methlycellulose and 2.5mg/mL rhodamine B dextran mixture (Invitrogen, D1841, MW: 70,000). After 15 minutes ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... and antibiotic-antimycotic (1:100 from 10,000 units/ml penicillin; 10,000 μg/ml streptomycin, 25 μg/ml amphotericin B, Gibco) mix at 37°C and 5% CO2 ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... and antibiotic-antimycotic (1:100 from 10,000 units/ml penicillin; 10,000 μg/ml streptomycin, 25 μg/ml amphotericin B, Gibco) mix at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... B-7-6 was produced in our own laboratory and was directly conjugated to DyLight (Pierce/Thermo Scientific) fluorochromes ...
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bioRxiv - Microbiology 2024Quote: ... Freshly isolated hepatocytes are suspended in complete William’s B medium (WB): William’s E with glutamax (ThermoFisher Scientific: 32551087), 1x Insulin/transferrin/selenium (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Biophysics 2021Quote: We previously cloned the genes for TEM and CTX-M-9 β-lactamase into pET24-b plasmids (Life Technologies) for inducible protein expression under the T7 promoter.[21 ...
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bioRxiv - Biophysics 2021Quote: ... 5 % CO2 incubator for 5 hours before replacing the culture media with 2 mL B-DMEM (Thermofisher, cat. # 10566016) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... from were prepared as reported previously (71) and maintained in Neurobasal media with B-27 supplements (Thermo Fisher Scientific). All animal procedures were approved by the Fred Hutchinson Cancer Research Center Institutional Animal Care and Use Committee ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Genomics 2019Quote: For the GFP tiling screen RfxCas13d-expressing cells were transduced with pLentiEGFPdestabilized lentivirus at low MOI (<0.1) and selected with 100μg/ml Hygromycin B (ThermoFisher 10687010) for 2 days ...
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bioRxiv - Immunology 2021Quote: ... which was supplemented with 10 mL of Antibiotic-Antimycotic (100 U/ml penicillin–100 μg/ml streptomycin–0.25 μg/ml amphotericin B; Gibco), 10% of fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Finally the cells were washed once with 0.5 mL Stellaris RNA wash buffer B and mounted with mounting solution (Invitrogen). All high resolution images were taken using confocal ZEISS LSM 880 in Airyscan super-resolution mode ...
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bioRxiv - Cancer Biology 2020Quote: ... and plated at a density of 1×106/mL in B-cell media (DMEM high glucose with Glutamax (Gibco), supplemented with Fetal Bovine Serum (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: HEK293T and U2OS or U2OS ATG2A/B double knock-out cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen 10313021) supplemented with 10% foetal bovine serum (FBS) ...
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bioRxiv - Immunology 2019Quote: ... Total splenocytes or isolated B cells were cultured using RPMI (with 10% decomplemented foetal calf serum, 1% penicillin 100Units/mL/streptomycin100μg/mL (Gibco), 50μM β-mercaptoethanol (PAN biotech) ...
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bioRxiv - Biochemistry 2019Quote: ... Positive screened samples were initiated to production by the addition of 20% CHO CD EfficientFeed B (Thermo Fisher, A1024001) and a temperature shift to 30°C ...
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bioRxiv - Microbiology 2019Quote: ... mosselii A1 and AA1 cells grown in Kings B or M63 broth using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... Solvent A was 0.1% formic acid in water and solvent B was 0.1% formic acid in acetonitrile (Fisher Scientific). The resulting electrospray (source voltage 1,800 V ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with the biotinylated secondary antibody (goat anti-mouse IgG Biotin, Life Technologies B-2763, 1:100) diluted in 0.1 % Triton X-100 in PBS for 1 h at RT in a humidified chamber ...
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bioRxiv - Neuroscience 2020Quote: ... Purification of T and B cells from total splenocytes was carried out by negative selection using Dynabeads untouched mouse T cells and Dynabeads untouched CD43 B cells isolation kits according to the manufacturer’s instruction (Invitrogen). Successful transfer of splenocytes was confirmed by flow cytometric analysis of blood and spleen at the conclusion of the study.
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bioRxiv - Bioengineering 2021Quote: ... and 1 v/v% penicillin/streptomycin/amphotericin B (1000 U/mL, 1000 μg/mL, and 0.25 μg/ mL final concentrations, respectively, Gibco). Prior to cell encapsulation ...
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bioRxiv - Microbiology 2021Quote: ... was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC) (Acros Organics, Belgium, USA) as described previously (64) ...
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bioRxiv - Biochemistry 2020Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (ThermoFisher). An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2.2% antibiotic/antimycotic (10,000 U/ml penicillin, 10,000 μg/ml streptomycin, and 25 μg/ml amphotericin B; Life Technologies). Supernatants collected in a fresh tube were centrifuged at 1000 rpm for 5 minutes and the pellet was resuspended in MEM containing 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... the unattached cells were discarded and attached cells were grown in MEM containing 10% FBS and 2.2% antibiotic/antimycotic (10,000 U/ml penicillin, 10,000 μg/ml streptomycin, and 25 μg/ml amphotericin B; Life Technologies) at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... we injected 40nl of cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (0.5%) or 647 (0.25%; Life Technologies) into the pulvinar ...
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bioRxiv - Neuroscience 2020Quote: ... A third monkey was injected with a cholera toxin subunit B-Alexa Fluor 647 conjugate (CTB-AF647; C34778, Invitrogen) at the quadrigeminal cistern ...
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bioRxiv - Neuroscience 2020Quote: ... and axial or intercostal muscles were injected with a solution of Cholera Toxin B Subunit-conjugated Alexa555 (Molecular Probes, Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Salivary glands from infected mosquitoes were hand dissected and collected in complete William’s B medium (William's E medium with Glutamax [Thermo Fisher, 32551-087] ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco), 50 mM 2-βmercaptoethanol (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... the U937 cells were selected with appropriate antibiotics (Zeocin 200µg/mL, G418 500 µg/mL, or Hygromycin B 200µg/mL; Thermo Fisher, Carl Roth ...
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bioRxiv - Cell Biology 2020Quote: ... GM1+ lipid rafts were stained with the cholera toxin-subunit B-AlexaFluor 647 conjugate (1:5000; Molecular Probes, C34778) (58) ...
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bioRxiv - Cell Biology 2020Quote: ... A flexible polydimethylsiloxane (PDMS, 10:1 ratio of Part A base: Part B curing agent) (Fisher Scientific, Cat. #NC9644388) reservoir is situated above the solenoid using optomechanical assembly rods (Thor Labs ...
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bioRxiv - Biophysics 2021Quote: ... LMNG followed by 20 column volume of Buffer B (25 mM HEPES pH 7.4, 150 mM NaCl) supplemented with 0.06% (w/v) Digitonin (ACROS Organics). TMEM120A was eluted in Buffer B with 0.06% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... The bacterial pellet containing induced GJA1-20k was lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) containing cOmplete ULTRA Protease Inhibitor Tablets (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... lungs were rinsed with antibiotics/antimycotics (10% P/S, 4% amphotericin-B [Sigma], 0.4% gentamicin [Gemini Bio] in PBS with Ca2+ and Mg2+ [Gibco]) followed by perfusion with 0.0035% Triton X-100 (American Bioanalytical) ...
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bioRxiv - Immunology 2022Quote: Blood was collected in collection tubes containing anticoagulant acid citrate dextrose (ADC) Solution B (Fisher Scientific, Hampton, NH, USA). Ficoll (Stemcell Technologies ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed with the ABsolute qPCR ROX Mix (AB-1138/B, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA) under the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed with the ABsolute qPCR ROX Mix (AB-1138/B, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA) under the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... The induced proteins were isolated from bacterial pellets lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) containing cOmplete ULTRA Protease Inhibitor Tablets (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Neural specific retrograde tracing was conducted with chloral toxin B (CTB-Alexa Fluor 488,10 mg/mL, Molecular Probes®), modulated pseudorabies virus vector (PRV) ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection was applied to transfectants for at least 5 passages prior to use: 150 µg/ml hygromycin-B (ThermoFisher) and 1 µg/ml puromycin (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Plant Biology 2021Quote: ... The OD at 645 nm and 663 nm for chlorophyll a and chlorophyll b were measured with NanoDrop (Thermofisher). The Chlorophyll concentration was calculated with the Arnon’s equations ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were lysed and inclusion bodies were purified using a B-Per protein purification kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...