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Citations for Thermo Fisher :
2301 - 2350 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and antibiotic/antimytotic cocktail (10 units/mL penicillin, 10 µg/mL streptomycin and 25 fg/mL Amphotericin B, Gibco). Ld652Y cells were originally derived from Lymantria dispar ovaries and have been found to be most sensitive to JcDV infection (18) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Neuroscience 2021Quote: ... RGC axons were anterogradely labeled by CTB (Cholera Toxin Subunit B) conjugated with Alexa Fluor™ 555 (Invitrogen, C34776) through intravitreal injection 48 hr before sacrifice ...
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bioRxiv - Microbiology 2020Quote: ... BCR-expressing Ramos B cells were suspended at 4 million cells/mL in RPMI++ and labeled with 1.5 μM Indo-1 (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Growth media had the same components as the transferring solution except it was supplemented with amphotericin B (ThermoFisher Scientific) to a concentration of 0.25μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Neuroscience 2022Quote: ... They were separated at 0.3 μL/minute flow rate under an increasing gradient of solvent B (0.1% FA in 95% acetonitrile) onto an EASY-Spray analytical column (75 μm × 50 cm, Thermo Scientific) packed with 2-μm C18 particles ...
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bioRxiv - Neuroscience 2022Quote: ... The neurons were maintained in the B-27™ Plus Neuronal Culture System (Thermo Fisher Scientific, cat. no. A3653401) that contained 2% B27 Plus and 1% PS ...
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bioRxiv - Neuroscience 2022Quote: ... and the pellet was resuspended in 5 mL B-PER™ complete bacterial protein extraction reagent (Thermo Scientific, #89821) supplemented with 2 mM DTT ...
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bioRxiv - Genetics 2022Quote: ... Transformation plates were then overlaid with 0.6% (w/v) water agar amended with 70 µg/mL hygromycin B (Invitrogen). Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies specific for IFN-γ (12-7319-42) and granzyme B (12-8896-42) were purchased from Thermofisher. Durvalumab (anti-PD-L1 antibody ...
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bioRxiv - Pathology 2020Quote: ... Each 3D-EHT was casted in a mold made of 2% agarose by adding 500,000 WTC11c hiPSC-CMs and 50,000 HS27a stromal cells in a fibrin gel solution (89 μL RPMI-1640 supplemented with B-27, 5.5 μl of DMEM/F12, [Gibco] ...
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bioRxiv - Biophysics 2019Quote: ... For optical tweezers experiments C-S10-B was labelled with Alexa Fluor™ 555 C2 Maleimide (Thermo Fisher Scientific) that binds to the (only present ...
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bioRxiv - Bioengineering 2020Quote: ... the plating media was aspirated and replaced with maintenance media containing Neurobasal growth medium supplemented with B-27 (Invitrogen), 1% 200 mM glutamine (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... An approximate total of 500-750 nL of Cholera toxin subunit B (CTB) AlexaFluor 488 or 647 Conjugate (Invitrogen) (Nanoject II ...
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bioRxiv - Neuroscience 2019Quote: ... A second retrograde tracer (Cholera toxin subunit B, Alexa Fluor 647 Conjugate, Invitrogen, 50–60 nl, 1.0 mg/mL) was injected elsewhere to label cells projecting to a different cortical or subcortical area ...
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bioRxiv - Biophysics 2019Quote: ... and 1% antibiotic–antimycotic (100 units/ml penicillin, 100 mg/ml streptomycin, and 0.25 mg/ml amphotericin B; Invitrogen), defined as DMEM-c ...
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bioRxiv - Microbiology 2019Quote: ... in acetonitrile (solvent B) in conjunction with a Syncronis™ HILIC LC column (100mm × 2.1mm x 2.1µm; Thermo Scientific). High-resolution mass spectral data were acquired on a Thermo Scientific Q-Exactive™ HF Hybrid Quadrupole-Orbitrap mass spectrometer coupled to an electrospray ionization source ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... buffer B: 0.2% formic acid and 5% DMSO in acetonitrile) in a Dionex Ultimate 3000 LC-system (Thermo Scientific). Peptides were then analyzed using a LTQ Orbitrap Elite mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... mice were mounted in a custom spine stabilizer and viral particles or Cholera Toxin Subunit B conjugated to Alexa Fluor 647 (CTB-647) in sterile 0.9% NaCl (C22841-Thermofisher, Waltham ...
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bioRxiv - Genetics 2021Quote: ... cells were inoculated from a pre-culture to 100 ml YES supplemented with Hygromycin B (100 μg/ mL, Invitrogen) and grown O/N at 30°C to OD600 0.4-0.8 ...
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bioRxiv - Genetics 2021Quote: ... the media was removed and replaced with fresh Neural Induction Media (NIM) supplemented with B-27 (Gibco, 17504-044), CultureOne (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Hygromycin-resistant transgenic worms were maintained on OP50-seeded NGM plates containing 0.2 mg/ml hygromycin B (Thermo Fisher). When large populations of aged-matched worms of such strains were required ...
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bioRxiv - Microbiology 2020Quote: ... with 20% Penicillin Streptomycin: Amphotericin B solution Cell Culture Reagent 100X (# 091674049) and 100X GlutaMax supplement by Gibco (# 35050061) was added the following day and transferred to 28°C ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Cell Biology 2021Quote: ... Drosophila S2 cells stably transfected with pMT-FMR1-ADARcd-V5 (see below) were grown in Schneider’s supplemented with 300 μg/mL hygromycin B (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... Finally the cells were washed once with 0.5 mL Stellaris RNA wash buffer B and mounted with mounting solution (Invitrogen). All high resolution images were taken using confocal ZEISS LSM 880 in Airyscan super-resolution mode ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining for Foxp3 and Granzyme B was performed with the Foxp3 staining kit (eBiocience, ThermoFisher Scientific, Langenselbold, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Total blood B-cells were then negatively enriched >95% by an immunomagnetic based technology (Dynabeads Untouched Invitrogen Life technologies). Total B-cells were subsequently cultured at a concentration of 106 cells/ml in IMDM supplemented with 10−4 β-2-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... Total blood B-cells were then negatively enriched >95% by an immunomagnetic based technology (Dynabeads Untouched Invitrogen Life technologies). Total B-cells were subsequently cultured at a concentration of 106 cells/ml in IMDM supplemented with 10−4 β-2-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... or in Neural stem cell media (NSC) based on DMEM-F12 supplemented with 50X B-27 (17504-044, Gibco), 100X N-2 (17502-048 ...
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted] ...
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bioRxiv - Immunology 2022Quote: ... only and those for B-cell ELISpot analysis were resuspended in 3mL RPMI 1640 medium GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% heat-inactivated horse serum (Gibco™ ...
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bioRxiv - Immunology 2022Quote: ... Raji-GFP-Luc cells were purchased from Biocytogen (B-HCL-010) and cultured in complete RPMI (21875, Thermo Fisher). Jurkat cells purchased from ATCC (TIB-152 ...
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bioRxiv - Microbiology 2022Quote: ... Briefly, inner wells (rows B–G, columns 2–11) of a 96-well flat-bottomed microtiter plate (ThermoFisher Scientific) were filled with 75μL 7H9-supplemented media ...
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bioRxiv - Microbiology 2022Quote: ... cells were loaded with fluorescent substrate CCF4-AM in a loading solution (LiveBLAzer-FRET B/G loading kit; Invitrogen) supplemented with 1 mM probenecid (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... which was supplemented with Antibiotic-Antimycotic (100 U/ml penicillin–100 μg/ml streptomycin–0.25 μg/ml amphotericin B; Gibco), 10% of fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Maintenance medium (Neurobasal medium [Thermo Fisher Scientific/Gibco; 21103–049] supplemented with 2% B-27 [Thermo Fisher Scientific/Gibco; 17504–044] ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to spheroid media containing Neurobasal-A media supplemented with B-27 without vitamin A (Invitrogen), Glutamax (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were passaged into 10 cm plates in 2i media containing 500 ng/mL Hygromycin-B (Gibco 10687010). After 2-4 days of selection ...
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bioRxiv - Immunology 2022Quote: ... The final weight of the bacteria pellet was measured and then the lysing reagent B-PER (Thermo Fisher Scientific) was added at a concentration of 4 ml/g of bacteria in combination with phenylmethylsufonyl fluoride (PMSF ...
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bioRxiv - Molecular Biology 2024Quote: ... Buffer A consisted of mass spectrometry grade water with 0.1% FA and buffer B consisted of acetonitrile with 0.1% FA (ThermoFisher Scientific). All samples were trapped for 5 minutes at a flow rate of 5 µL/min using 99% buffer A and 1% buffer B on a Symmetry BEH C18 Trapping Column (5 mm ...
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bioRxiv - Molecular Biology 2024Quote: ... The differentiation was initiated by removing mTeSR1 medium and adding RPMI/B-27 without insulin (#A1895601, Thermo Fisher Scientific) and containing 6 µM of CHIR99021 (#04-0004 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1x Antibiotic-Antimycotic (100 units/mL of penicillin, 100µg/mL of streptomycin, and 0.25 µg/mL of Amphotericin B, Thermo Fisher) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Evotips were activated with 25 µl of Evosep solvent B (0.1% formic acid in acetonitrile, Thermo Fisher Scientific, USA), followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in 0.01x buffer B for 1 min before mounting the coverslips with ProLong Diamond Antifade Mountant (Invitrogen).