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Citations for Thermo Fisher :
2351 - 2400 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The differentiation was initiated by removing mTeSR1 medium and adding RPMI/B-27 without insulin (#A1895601, Thermo Fisher Scientific) and containing 6 µM of CHIR99021 (#04-0004 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1x Antibiotic-Antimycotic (100 units/mL of penicillin, 100µg/mL of streptomycin, and 0.25 µg/mL of Amphotericin B, Thermo Fisher) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Evotips were activated with 25 µl of Evosep solvent B (0.1% formic acid in acetonitrile, Thermo Fisher Scientific, USA), followed by 2 min incubation in 2-propanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in 0.01x buffer B for 1 min before mounting the coverslips with ProLong Diamond Antifade Mountant (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... These selected colonies were grown in King’s B medium consisting of 20 g l-1 Bacto proteose peptone No.3 (Gibco), 1.5 g l-1 Potassium phosphate dibasic trihydrate (P5504 ...
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bioRxiv - Immunology 2024Quote: Relevant selective drugs were added to the medium at the following concentrations: 200 μg/mL hygromycin B (Invitrogen, USA), 30 μg/mL puromycin dihydrochloride (Sigma) ...
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bioRxiv - Immunology 2024Quote: The acute B-lymphocytic leukemia cell line Nalm6 was cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... to produce tandem mass spectra whose b and y ion series patterns were compared to Sequest (Thermo Fisher Scientific) to establish protein identity ...
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bioRxiv - Microbiology 2023Quote: ... Vero cells stably co-expressing human ACE2 and TMPRSS2 (Vero-ACE2/TMPRSS2) (gifted from A. Creanga and B. Graham at NIH) were maintained in Dulbecco’s Modified Eagle medium (DMEM; Gibco) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco], Maintenance Cocktail B [Gibco], and 0.1µM Dexamethasone [Gibco]) for one day ...
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bioRxiv - Microbiology 2023Quote: ... washed twice with PBS and loaded with CCF4-AM (LiveBLAzer™ FRET-B/G Loading Kit, Life Technologies; K1095), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were centrifuged and resuspended to an OD600 = 25.0 in B-PER™ Complete Bacterial Protein Extraction Reagent (Thermofisher) with 1mM PMSF and 38 μg/mL lysozyme and incubated at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer A consisted of mass spectrometry grade water with 0.1% FA and buffer B consisted of acetonitrile with 0.1% FA (ThermoFisher Scientific). All samples were trapped for 5 minutes at a flow rate of 5 μL/min using 99% buffer A and 1% buffer B on a Symmetry BEH C18 Trapping Column (5 mm ...
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bioRxiv - Biochemistry 2023Quote: ... For antibiotic selection complete medium (GibcoTM DMEM + 10 % FBS + 1 % PenStrep) supplemented with hygromycin B (0.7 mg/mL, Invitrogen) was added and exchanged 2-3 times a week for stable integration of the PB plasmid into the genome.
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bioRxiv - Immunology 2023Quote: ... Ganglioside M1 (GM1) staining was performed using recombinant Choleratoxin B labelled with AlexaFluor647 (3 µg/ml, Thermo Fisher, #C34778). Both GFP-D4 and AlexaFluor647-labelled CTB were added to FACS buffer-washed cells and incubated for 20 minutes at 4°C in the dark ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... Rtn4B was stained with 10μg/ml of a Nogo-B antibody (AF6034, BioTechne) followed by Alexa Fluor 647 secondary antibody (A-21448, Invitrogen) diluted 1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in serum-free neurobasal media supplemented with N-2 and B-27 Plus Supplements (Gibco™), 3 mM Gibco® GlutaMAX™ Supplement ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and 2 mM glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and M-CSF (100ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... organoids were passaged using H+++B Medium (Advanced DMEM/F-12 [Thermo Fisher], HEPES pH7.2-7.5 [10mM, Thermo Fisher] ...
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bioRxiv - Microbiology 2023Quote: ... Vero cells stably co-expressing human ACE2 and TMPRSS2 (Vero-ACE2/TMPRSS2) (gifted from A. Creanga and B. Graham at NIH) were maintained in Dulbecco’s Modified Eagle medium (DMEM; Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Retrograde tracers were cholera toxin subunit B (CTB-488; Alexa 488; Molecular probe, Invitrogen, 0.5% in HEPES buffered saline), WGA-Alexa488 (Thermo Fisher Scientific ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 15 min in 0.01% buffer B and a 1/10,000 dilution of DAPI (Life Technologies) for nuclei staining ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 μL of cholera toxin subunit B (CTB) conjugated with Alexa Fluor 594 (2 mg/ml, Thermo Fisher Scientific) was injected into the right vitreous humors of the same mice (same procedure as viral injection ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were PCR amplified and ligated into the copper-inducible pMT/V5-His B vector (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... 0.5% acetic acid) and solvent B (80% acetonitrile, 0.5% acetic acid) into an Orbitrap Eclipse mass spectrometer (Thermo Scientific). High resolution full MS spectra were acquired with a resolution of 120,000 ...
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bioRxiv - Physiology 2023Quote: ... and separated with a binary buffer system consisting of buffer A (0.1% FA) and buffer B (0.1% FA in 80% ACN) with an EASY-NLC 1,200 (Thermo Fisher Scientific). The LC system was directly coupled online with the mass spectrometer (Exploris 480 ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL bovine serum albumin and antibiotic-antimycotic 1:100 (10,000 units/mL penicillin; 10,000 μg/mL streptomycin; 25 μg/mL amphotericin B)) (ThermoFisher Scientific). Cortices were cut into small pieces and digested in 5.0 mL sterile filtered papain solution for 15 minutes at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Human immortalised endometrial epithelial cells (Ishikawa cells) were cultured at 37°C (5% CO2) in DMEM:F12 B-Nut mix (50:50, ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2023Quote: ... Each mouse received a series of injections of Alexa Fluor 488- conjugated cholera toxin B (CTB) (Invitrogen; C-34775). These injections were into the subserosa of the distal colon ...
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bioRxiv - Microbiology 2023Quote: ... Briefly, inner wells (rows B–G, columns 3–11) of a 96-well flat-bottomed microtiter plate (ThermoFisher Scientific) were filled with 75 µl 7H9 media ...
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bioRxiv - Immunology 2023Quote: ... and APC-coupled anti-HLA-A/B/C/E Ab (RRID:AB_314879) in PBS/BSA with 1% saponin (ThermoFisher #C10424) prior to acquisition on a BD LSRFortessa.
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bioRxiv - Molecular Biology 2023Quote: ... Worms were obtained as described above and cultured in RPMI medium (supplemented with 5% chicken serum and 1% ABAM-solution [10,000 units penicillin, 10 mg streptomycin, and 25 mg amphotericin B per ml], all from Gibco) at 37 °C in a 5% CO2 atmosphere for 72 h ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were gavaged with a 2% methylcellulose mixture containing 2.5 mg/mL Rhodamine B Dextran (Invitrogen, D1841, MW: 70,000). 15 minutes after gavage ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and 2 mM glutamine ...
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bioRxiv - Microbiology 2023Quote: ... HeLa cells stably expressing TRIM fusions were generated by transduction with LHCX-derived vectors followed by selection in 100μg ml−1 hygromycin B (Gibco). Vector stocks for transduction were generated by co-transfection of 293T cells with a VSV-G expression plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... following the manufacture recommendations and 72 hours post-transfection cells selected with 350 μg/mL of Hygromicin B (Gibco) for 5 days ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... a solution containing cholera toxin subunit B (CTb; 1.0 mg/mL) conjugated to Alexa 488 or Alexa 555 (Invitrogen) as a bidirectional tracer was injected into the aDLS and pVLS (0.3 μL/site) ...
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bioRxiv - Molecular Biology 2024Quote: ... We designed two FRG2A/B-specific siRNAs (Supplemental Figure 16 for sequences and localization) and silencer select siRNA (Invitrogen Silencer™ Select Pre-Designed siRNA Catalog number:4392420 ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REx 293 cell lines with reconstituted PKR were maintained in 5% FBS DMEM supplemented with 50µg/mL hygromycin B (Invitrogen) and 15µg/mL blasticidin ...
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bioRxiv - Synthetic Biology 2024Quote: ... frozen at -20°C and resuspended in 4 mL/gr of pellet of B-PER Reagent (Thermo Fisher Scientific) with 2 µl of lysozyme (50 mg/mL ...
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bioRxiv - Plant Biology 2019Quote: ... pEF1-MCS-3Myc [BamHI-NotI-3Myc-stop fragment was inserted between KpnI and XbaI sites of pEF1/myc-His B vector (Invitrogen)] was generated ...
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bioRxiv - Neuroscience 2021Quote: ... the media was replaced and maintained with neurobasal media supplemented with B-27 (cat no. 17504-044, Invitrogen, Carlsbad CA), 1% Penicillin/Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... gradient of 2% to 25% buffer B (0.1% formic acid in acetonitrile) was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). The ion source was operated at 2.4kV and the ion transfer tube was set to 300°C ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids were selected for integration of constructs in organoid media containing Wnt3A conditioned media (Urbischek et al., 2019) supplemented with 150 μg/ml Hygromycin B (ThermoFisher) for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... Filtered viral supernatant media were added to dishes containing SBM044 and SBM046 and 48 hours later hygromycin B (Invitrogen 10687010) was added at 400 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC, 20 µM) and CellTracker orange (CMTMR, 10 µM; both Invitrogen) respectively ...
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bioRxiv - Microbiology 2020Quote: ... Extracted type VI-B PCR-products were re-amplified using the Maxima Hot Start Taq DNA polymerase (Thermo Fisher Scientific), M13-B185_223bp_C2F and P1-B185_223bp_C2R primers ...